TY  - JOUR
TI  - The Effects of Rutaecarpine on Metastatic Prostate  Cancer Cells
AB  - Prostate cancer with an increased incidence in the world is one of the public health-threatening malignancy. Metastatic prostate cancer is an important cause of death in men despite of the combined use of more than one chemotherapeutic drug as well as radiotherapy and supportive treatments. Therefore, there is a need to de velop novel treatment strategies in  metastatic prostate cancer patients. The aim of this study was to investigate the potential therapeutic effects of Rutaecarpine (RUT) on metastatic prostate cancer cells. RUT induced cytotoxicity and apoptotic cell death were evaluated  by WST-1, Annexin V, AO staining and ELISA assays in PC-3 human metastatic prostate cancer cell line.The viability percentage of PC-3 cells after exposing to different concentrations of RUT treatment significantly decreased  in a time and dose dependent manner and the most effective concentrations of RUT was determined as 20 and 40 μM for 48 hours (p<0.05). Annexin V and AO staining revealed that the early and late apoptosis rate significantly increased compared to the control group (p <0.05). Additionally, the caspase -3 levels significantly increased after RUT treatment in PC-3 cells in a dose dependent manner (p<0.05). In this study, RUT exhibited a cytotoxic and apoptotic effects on PC-3 cells and therefore RUT could be a potential new therapeutic agent for the treatment of metastatic prostate cancer.  However, the underlying mechanism of the apoptotic death caused by RUT in PC-3 cells should be further investigated through advanced analysis at molecular level.
AU  - ÖZMAN, Zeynep
AU  - Şekeroğlu, Mehmet Abdulkadir
AU  - Nogay, Fatıma Betül
AU  - guney eskiler, gamze
AU  - Şekeroğlu, Mehmet Ramazan
AU  - ÇOKLUK, erdem
DO  - 10.5505/ejm.2022.10437
PY  - 2022
JO  - Eastern Journal of Medicine
VL  - 27
IS  - 2
SN  - 1301-0883
SP  - 302
EP  - 306
DB  - TRDizin
UR  - http://search/yayin/detay/1124330
ER  -