Objective: The aim of this study was to assess the interactive training on placenta and umbilical cord given to midwives who provide care for pregnant women and newborns. Methods: The midwives accepted the invitation for interactive presentation before the training. The introductory information of participants was collected by a questionnaire form prepared by researchers. Assessment of the knowledge of the participants on placenta and umbilical cord was performed by using a 24-question questionnaire form prepared by the researchers in accordance with the literature. A pre-test was conducted before the training. The participants were not informed whether the test would be repeated at first and third months after the training or not. The test was repeated 1 and 3 months later. Friedman test was used in the comparison of the mean values of more than two dependent groups as the data did not provide parametric characteristics, and Kruskall-Wallis test was used for the comparison of two groups. Statistically significant level was considered p<0.05 for all the data. Results: The mean age of midwives included in the study was 37.23±6.19 (range: 25 to 51) years, 52.9% of them had bachelor’s degree, 88.2% of them were married, their total experience in their profession was 15.88±6.90 years and they were working in the same unit for 7.5 (range: 1 to 26) years. Of the participants, the mean pretest score was 12.88±2.15, and mean final test scores 1 month and 3 months later were 21.61±1.75 and 23.23±0.85, respectively. Conclusion: Interactive training given on placenta and umbilical cord provides a positive contribution to increase the knowledge score of midwives. It may be suggested to repeat in-service trainings in the organization regularly.