Objective Feeding with breast milk (BM) is important in reducing infant mortality and morbidity. Compliance with hygiene rules when preparing BM or preparing for breastfeeding is important in preventing infections that may develop. The aim of this study was to investigate the satisfaction of mothers after breastfeeding and hygiene education (BFHE) in the neonatal intensive care unit (NICU). Materials and Methods This study was conducted between January 1, 2016 and January 1, 2017 at Sakarya University Training and Research Hospital NICU. Ethical approval of the study was obtained from the interventional ethics committee of Sakarya University Faculty of Medicine. Mothers of patients in the NICU were given BFHE video training. After the training, volunteer mothers were asked to fill out the interview form. Demographic questions about the age and education of the mother and questions about the satisfaction of the video training were asked in the interview form. In the satisfaction level assessment, the lowest satisfaction score was 0 and the highest satisfaction score was 10. The obtained data were analyzed in SPSS 21 program, p <0,05 was considered significant. Results The mean age of the 538 mothers included in the study was 28,1 ± 5,7 (17-45) years. When the distribution of the mothers according to their educational status was evaluated; 269 (50.00%) of them were primary school, 206 (38.28%) of them were high school and 63 (11.72%) were university graduates. Mean satisfaction level of mothers was 9,75 ± 0,94 (0-10.00). When the age and satisfaction level of the mothers were evaluated, it was determined that as the age increased, their satisfaction increased (p = 0,049, r = 0,085). Conclusion Mothers can be trained with simple trainings and standardized trainings can be used to improve the quality of mother and child care.