Screening and Selection of Media Components for Protease Production by Bacillus sp. EBTA6 Using Plackett–Burman Design
Yıl: 2020 Cilt: 8 Sayı: 6 Sayfa Aralığı: 1250 - 1255 Metin Dili: İngilizce DOI: 10.24925/turjaf.v8i6.1250-1255.2986 İndeks Tarihi: 06-06-2021
Screening and Selection of Media Components for Protease Production by Bacillus sp. EBTA6 Using Plackett–Burman Design
Öz: In this study, effects of medium components and inoculum size on the protease production by Bacillussp. EBTA6 that was isolated from a home-made Tarhana sample were investigated. The cell-freesupernatant of bacterium cultured on a shaking incubator for 24 h was used to test protease activityas the response. With a total number of 11 factors, 12 different experiments were run and the highestexperimental protease activity was measured as 2280.4 U/mL. Results were analyzed statistically byANOVA and the most efficient factors were detected as yeast extract, dipotassium phosphate, casein,and peptone with a contribution of 93.78, 2.19, 1.96, 1.31%, respectively. For validation of theselected factors, a further experiment was performed by using of yeast extract (9.98 g/L), dipotassiumphosphate (1.27 g/L), casein (8.69 g/L), and peptone (9.88 g/L) obtained from the design equation.The experimental response was found as 2411.4 U/mL which was only 5.5% higher than the predictedresponse showing that the model was applicable.
Anahtar Kelime: Bacillus sp. EBTA6 ile Proteaz Üretimi için Plackett–Burman Dizaynı Kullanılarak Besiyeri Bileşenlerinin Taranması ve Seçimi
Öz: Bu çalışmada, Plackett–Burman Dizaynı kullanılarak ev yapımı bir Tarhanadan izole edilen Bacillus sp. EBTA6’nın proteaz üretimine besiyeri bileşenleri ve inokülüm miktarının etkisi araştırılmıştır. Yanıt olarak proteaz aktivitesinin belirlenmesi amacıyla çalkalamalı inkübatörde 24 saat geliştirilen bakterinin hücresiz süpernatantı kullanılmıştır. Toplam 11 faktör ile 12 farklı deneme gerçekleştirilmiş ve en yüksek deneysel proteaz aktivitesi 2280,4 U/mL olarak ölçülmüştür. Sonuçlar ANOVA ile istatistiki olarak analiz edilmiş ve en etkili faktörler %93,78; 2,19; 1,96; 1,31 katkı oranları ile sırasıyla maya özütü, dipotasyum fosfat, kazein ve pepton olarak tespit edilmiştir. Seçilen faktörlerin doğrulanması için dizayn denkleminden elde edilen maya özütü (9,98 g/L), dipotasyum fosfat (1,27 g/L), kazein (8,69 g/L) ve pepton (9,88 g/L) ile de bir deneme gerçekleştirilmiştir. Deneysel yanıt, tahmin edilen yanıttan yalnızc
Anahtar Kelime: Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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| APA | AKÇAY F, Avci A (2020). Screening and Selection of Media Components for Protease Production by Bacillus sp. EBTA6 Using Plackett–Burman Design. , 1250 - 1255. 10.24925/turjaf.v8i6.1250-1255.2986 |
| Chicago | AKÇAY FİKRİYE ALEV,Avci Ayse Screening and Selection of Media Components for Protease Production by Bacillus sp. EBTA6 Using Plackett–Burman Design. (2020): 1250 - 1255. 10.24925/turjaf.v8i6.1250-1255.2986 |
| MLA | AKÇAY FİKRİYE ALEV,Avci Ayse Screening and Selection of Media Components for Protease Production by Bacillus sp. EBTA6 Using Plackett–Burman Design. , 2020, ss.1250 - 1255. 10.24925/turjaf.v8i6.1250-1255.2986 |
| AMA | AKÇAY F,Avci A Screening and Selection of Media Components for Protease Production by Bacillus sp. EBTA6 Using Plackett–Burman Design. . 2020; 1250 - 1255. 10.24925/turjaf.v8i6.1250-1255.2986 |
| Vancouver | AKÇAY F,Avci A Screening and Selection of Media Components for Protease Production by Bacillus sp. EBTA6 Using Plackett–Burman Design. . 2020; 1250 - 1255. 10.24925/turjaf.v8i6.1250-1255.2986 |
| IEEE | AKÇAY F,Avci A "Screening and Selection of Media Components for Protease Production by Bacillus sp. EBTA6 Using Plackett–Burman Design." , ss.1250 - 1255, 2020. 10.24925/turjaf.v8i6.1250-1255.2986 |
| ISNAD | AKÇAY, FİKRİYE ALEV - Avci, Ayse. "Screening and Selection of Media Components for Protease Production by Bacillus sp. EBTA6 Using Plackett–Burman Design". (2020), 1250-1255. https://doi.org/10.24925/turjaf.v8i6.1250-1255.2986 |
| APA | AKÇAY F, Avci A (2020). Screening and Selection of Media Components for Protease Production by Bacillus sp. EBTA6 Using Plackett–Burman Design. Türk Tarım - Gıda Bilim ve Teknoloji dergisi, 8(6), 1250 - 1255. 10.24925/turjaf.v8i6.1250-1255.2986 |
| Chicago | AKÇAY FİKRİYE ALEV,Avci Ayse Screening and Selection of Media Components for Protease Production by Bacillus sp. EBTA6 Using Plackett–Burman Design. Türk Tarım - Gıda Bilim ve Teknoloji dergisi 8, no.6 (2020): 1250 - 1255. 10.24925/turjaf.v8i6.1250-1255.2986 |
| MLA | AKÇAY FİKRİYE ALEV,Avci Ayse Screening and Selection of Media Components for Protease Production by Bacillus sp. EBTA6 Using Plackett–Burman Design. Türk Tarım - Gıda Bilim ve Teknoloji dergisi, vol.8, no.6, 2020, ss.1250 - 1255. 10.24925/turjaf.v8i6.1250-1255.2986 |
| AMA | AKÇAY F,Avci A Screening and Selection of Media Components for Protease Production by Bacillus sp. EBTA6 Using Plackett–Burman Design. Türk Tarım - Gıda Bilim ve Teknoloji dergisi. 2020; 8(6): 1250 - 1255. 10.24925/turjaf.v8i6.1250-1255.2986 |
| Vancouver | AKÇAY F,Avci A Screening and Selection of Media Components for Protease Production by Bacillus sp. EBTA6 Using Plackett–Burman Design. Türk Tarım - Gıda Bilim ve Teknoloji dergisi. 2020; 8(6): 1250 - 1255. 10.24925/turjaf.v8i6.1250-1255.2986 |
| IEEE | AKÇAY F,Avci A "Screening and Selection of Media Components for Protease Production by Bacillus sp. EBTA6 Using Plackett–Burman Design." Türk Tarım - Gıda Bilim ve Teknoloji dergisi, 8, ss.1250 - 1255, 2020. 10.24925/turjaf.v8i6.1250-1255.2986 |
| ISNAD | AKÇAY, FİKRİYE ALEV - Avci, Ayse. "Screening and Selection of Media Components for Protease Production by Bacillus sp. EBTA6 Using Plackett–Burman Design". Türk Tarım - Gıda Bilim ve Teknoloji dergisi 8/6 (2020), 1250-1255. https://doi.org/10.24925/turjaf.v8i6.1250-1255.2986 |
