TY  - JOUR
TI  - Screening and Selection of Media Components for Protease Production by Bacillus sp. EBTA6 Using Plackett–Burman Design
AB  - In this study, effects of medium components and inoculum size on the protease production by Bacillussp. EBTA6 that was isolated from a home-made Tarhana sample were investigated. The cell-freesupernatant of bacterium cultured on a shaking incubator for 24 h was used to test protease activityas the response. With a total number of 11 factors, 12 different experiments were run and the highestexperimental protease activity was measured as 2280.4 U/mL. Results were analyzed statistically byANOVA and the most efficient factors were detected as yeast extract, dipotassium phosphate, casein,and peptone with a contribution of 93.78, 2.19, 1.96, 1.31%, respectively. For validation of theselected factors, a further experiment was performed by using of yeast extract (9.98 g/L), dipotassiumphosphate (1.27 g/L), casein (8.69 g/L), and peptone (9.88 g/L) obtained from the design equation.The experimental response was found as 2411.4 U/mL which was only 5.5% higher than the predictedresponse showing that the model was applicable.
AU  - Avci, Ayse
AU  - AKÇAY, FİKRİYE ALEV
DO  - 10.24925/turjaf.v8i6.1250-1255.2986
PY  - 2020
JO  - Türk Tarım - Gıda Bilim ve Teknoloji dergisi
VL  - 8
IS  - 6
SN  - 2148-127X
SP  - 1250
EP  - 1255
DB  - TRDizin
UR  - http://search/yayin/detay/418620
ER  -