An Investigation on Some Toxic Effects of Carbofuran on Daphnia magna (Crustacea, Cladocera)
Yıl: 2016 Cilt: 44 Sayı: 2 Sayfa Aralığı: 155 - 160 Metin Dili: İngilizce DOI: 10.15671/HJBC.20164418123 İndeks Tarihi: 08-02-2022
An Investigation on Some Toxic Effects of Carbofuran on Daphnia magna (Crustacea, Cladocera)
Öz: The aim of this study was to determine the median effective concentration ( $EC_{50}$) of carbofuran, a carbamate pesticide, for Daphnia magna and to evaluate the effects on the heart rate. In the experiment, five neonates (≤ 24 hours) were used for each negative control, solvent control (acetone) and the treatment groups. The treatment groups were exposed to five different concentrations (2.5 µg/L, 4.5 µg/L, 8.1 µg/L, 14.58 µg/L and 26.244 µg/L) of carbofuran (98%) for 48 hours. Immobilised daphnids were noted at the 24th and the 48th hour in both control and treatment groups. $EC_{50}$ values were calculated by probit analysis as 33.814 µg/L and 3.451 µg/L for 24 and 48 h, respectively. At the end of the 48th h, all samples were observed by light microscopy and the heart beats per minute (bpm) were counted by using a video camera. Heart rate was similar in negative and solvent control groups (444.8±24.12 and 458.4±34.14, respectively). In the treatment groups heart beats were noted as 498.4±17.43, 183.8±103.83, 441.6±35.73, 293.8±101.09 and 442.4±32.94 for 2.5, 4.5, 8.1, 14.58 and 26.244 µg/L, respectively. These results show that carbofuran caused immobilisation and decreased heart rate in D. magna. However, heart occured independently of carbofuran concentration gradient.
Anahtar Kelime: Karbofuran’ın Daphnia magna (Crustacea, Cladocera) Üzerindeki Bazı Toksik Etkilerinin Araştırılması
Öz: Bu çalışmada karbamatlı bir pestisit olan karbofuran’ın Daphnia magna için $EC_{50}$ değerinin bulunması ve kalp atım sayısı üzerindeki etkilerinin belirlenmesi amaçlanmıştır. Denemede negatif kontrol, çözücü kontrol (aseton) ve uygulama grupları için beşer daphnid (24 saatlikten küçük) kullanılmıştır. Uygulama grupları karbofuran’ın (% 98 saflıkta) beş farklı derişimine (2.5 µg/L, 4.5 µg/L, 8.1 µg/L, 14.58 µg/L ve 26.24 µg/L) 48 saat boyunca maruz bırakılmıştır. Tüm gruplarda 24. ve 48. saatlerde hareketsiz bireyler kaydedilmiş ve probit analiz yöntemi ile $EC_{50}$ değerleri sırasıyla 33.814 µg/L ve 3.451 µg/L olarak hesaplanmıştır. 48 saat sonunda, tüm örnekler ışık mikroskobu ile incelenmiş ve bir dakikadaki kalp atım sayıları video kamera ile kaydedilerek belirlenmiştir. Kalp atım sayıları negatif kontrol grubunda 444.8±24.12; çözücü uygulanan kontrol grubunda ise 458.4±34.14 olarak birbirine yakın değerlerdedir. Deneme gruplarında bu sayılar artan derişimine göre sırasıyla 2.5 µg/L için 498.4±17,43; 4.5 µg/L için 183.8±103.83; 8,1 µg/L için 441.6±35.73; 14.58 µg/L için 293.8±101.09 ve 26.244 µg/L için 442.4±32.94 olarak kaydedilmiştir. Buna göre karbofuran, D. magna neonatlarında hareketsizliğe ve kalp atım sayılarında azalmaya sebep olmuştur; ancak bu azalma derişim artışı ile paralellik göstermemektedir
Anahtar Kelime: Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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| APA | SOMUNCU S, AKAT E, ÜÇÜNCÜ S (2016). An Investigation on Some Toxic Effects of Carbofuran on Daphnia magna (Crustacea, Cladocera). , 155 - 160. 10.15671/HJBC.20164418123 |
| Chicago | SOMUNCU Sezgi,AKAT Esra,ÜÇÜNCÜ Sema İşisağ An Investigation on Some Toxic Effects of Carbofuran on Daphnia magna (Crustacea, Cladocera). (2016): 155 - 160. 10.15671/HJBC.20164418123 |
| MLA | SOMUNCU Sezgi,AKAT Esra,ÜÇÜNCÜ Sema İşisağ An Investigation on Some Toxic Effects of Carbofuran on Daphnia magna (Crustacea, Cladocera). , 2016, ss.155 - 160. 10.15671/HJBC.20164418123 |
| AMA | SOMUNCU S,AKAT E,ÜÇÜNCÜ S An Investigation on Some Toxic Effects of Carbofuran on Daphnia magna (Crustacea, Cladocera). . 2016; 155 - 160. 10.15671/HJBC.20164418123 |
| Vancouver | SOMUNCU S,AKAT E,ÜÇÜNCÜ S An Investigation on Some Toxic Effects of Carbofuran on Daphnia magna (Crustacea, Cladocera). . 2016; 155 - 160. 10.15671/HJBC.20164418123 |
| IEEE | SOMUNCU S,AKAT E,ÜÇÜNCÜ S "An Investigation on Some Toxic Effects of Carbofuran on Daphnia magna (Crustacea, Cladocera)." , ss.155 - 160, 2016. 10.15671/HJBC.20164418123 |
| ISNAD | SOMUNCU, Sezgi vd. "An Investigation on Some Toxic Effects of Carbofuran on Daphnia magna (Crustacea, Cladocera)". (2016), 155-160. https://doi.org/10.15671/HJBC.20164418123 |
| APA | SOMUNCU S, AKAT E, ÜÇÜNCÜ S (2016). An Investigation on Some Toxic Effects of Carbofuran on Daphnia magna (Crustacea, Cladocera). Hacettepe Journal of Biology and Chemistry, 44(2), 155 - 160. 10.15671/HJBC.20164418123 |
| Chicago | SOMUNCU Sezgi,AKAT Esra,ÜÇÜNCÜ Sema İşisağ An Investigation on Some Toxic Effects of Carbofuran on Daphnia magna (Crustacea, Cladocera). Hacettepe Journal of Biology and Chemistry 44, no.2 (2016): 155 - 160. 10.15671/HJBC.20164418123 |
| MLA | SOMUNCU Sezgi,AKAT Esra,ÜÇÜNCÜ Sema İşisağ An Investigation on Some Toxic Effects of Carbofuran on Daphnia magna (Crustacea, Cladocera). Hacettepe Journal of Biology and Chemistry, vol.44, no.2, 2016, ss.155 - 160. 10.15671/HJBC.20164418123 |
| AMA | SOMUNCU S,AKAT E,ÜÇÜNCÜ S An Investigation on Some Toxic Effects of Carbofuran on Daphnia magna (Crustacea, Cladocera). Hacettepe Journal of Biology and Chemistry. 2016; 44(2): 155 - 160. 10.15671/HJBC.20164418123 |
| Vancouver | SOMUNCU S,AKAT E,ÜÇÜNCÜ S An Investigation on Some Toxic Effects of Carbofuran on Daphnia magna (Crustacea, Cladocera). Hacettepe Journal of Biology and Chemistry. 2016; 44(2): 155 - 160. 10.15671/HJBC.20164418123 |
| IEEE | SOMUNCU S,AKAT E,ÜÇÜNCÜ S "An Investigation on Some Toxic Effects of Carbofuran on Daphnia magna (Crustacea, Cladocera)." Hacettepe Journal of Biology and Chemistry, 44, ss.155 - 160, 2016. 10.15671/HJBC.20164418123 |
| ISNAD | SOMUNCU, Sezgi vd. "An Investigation on Some Toxic Effects of Carbofuran on Daphnia magna (Crustacea, Cladocera)". Hacettepe Journal of Biology and Chemistry 44/2 (2016), 155-160. https://doi.org/10.15671/HJBC.20164418123 |
