TY  - JOUR
TI  - Determination of the Apoptotic Effect of Raf2 and Nck-Interacting Protein Kinase Inhibitor on Metastatic Canine Mammary Gland Tumor Cells
AB  - Objective: Canine mammary gland tumors (CMGTs) are the most common tumors in female dogs and the main treatment options used in CMGTs are surgery caused some complications. Therefore, new treatment options are needed for the CMGTs. Traf2 and Nck-interacting serine protein kinase (TNIK) as a transcriptional coregulator of Wnt targeted genes is highly expressed in breast cancer development. The inhibition of TNIK may be a new therapeutic target in cancers with abnormal WNT signaling. Therefore we aimed to investigate the potential therapeutic effect of NCB-0846 on metastatic sarcoma CMGTs subtype, for the first time. Materials and Methods: The cytotoxic and apoptotic effects of NCB-0846 on CMGT cells were analyzed by WST‑1, Annexin V, cell cycle, acridine orange (AO) and DAPI staining. Results: NCB-0846 significantly inhibited cell viability in a dose and time dependent manner (p<0.05) and induced nuclear damage in CMGT cells. Furthermore, NCB-0846 caused apoptotic cell death through the accumulation of cells in the G0/G1 phase. Conclusion: Our findings demonstrated that NCB-0846 could be potentially a new therapeutic anti-cancer agent in the treatment of CMGTs. However, further investigations need to be performed in order to elucidate the inhibitory activity of TNIK and Wnt signaling by NCB-0846 on CMGT cells. 
AU  - Hacıefendi, Ayten
AU  - Deveci Özkan, Asuman
AU  - guney eskiler, gamze
AU  - ÖZKAN, Fatih
AU  - Cakmak, Ecir Ali
AU  - Kaleli, Prof. Dr. Suleyman
AU  - Turna, Ozge
DO  - 10.26453/otjhs.1036628
PY  - 2022
JO  - Online Türk Sağlık Bilimleri Dergisi
VL  - 7
IS  - 1
SN  - 2459-1467
SP  - 117
EP  - 122
DB  - TRDizin
UR  - http://search/yayin/detay/508185
ER  -