Aim: COVID-19 has caused concern to people and healthcare workers all over the world. Increasing number of cases and deaths appearing in the media has increased the anxiety levels of healthcare workers, especially related to their profession. This descriptive cross-sectional study was conducted to determine the occupational anxiety levels of the emergency department workers in a training and research hospital during the COVID-19 pandemic and to contribute to the literature by analyzing the related variables. Material and Methods: The population of the study consisted of the emergency department workers of a Training and Research Hospital. Data were collected by reaching 83.9% of the research population. The data of the research was obtained with the "Emergency Service Worker Information Form" and the "Occupational Anxiety Scale for Emergency Health Workers" using an online survey prepared via Google forms. Results: The mean age of the participants in the study was 33.71±8.13, and the mean number of years working in the emergency department was 7.90±6.89 years. We found that 86.2% of the emergency service workers came into contact with a Covid-19 positive patient, and 31.9% had contact for longer than eight hours. During the pandemic period, 90.4% of the emergency department workers were worried about work, and 21.3% wanted to quit their job. The mean scores of occupational anxiety scale of the personnel providing emergency health services was determined as 77.29±16.12. The occupational anxiety scale mean scores of female employees, singles, people who have no-children, working 24 hour shifts, doctors and nurses, and those who were worried about work during the pandemic period were found to be statistically significantly higher (p<0.05). Conclusion: We found that occupational anxiety level of the emergency service workers was moderate. Although there was no difference between the occupational anxiety levels experienced by doctors and nurses, it was found to be higher than other occupational groups. The high level of professional anxiety of doctors and nurses can be explained by the fact that they spend more time in patient care.We suggest that professional anxiety should be among the priority issues that need to be resolved by giving the necessary support administratively.