The aim of this study is to determine the universal science literacy levels of the science teachers who work in state secondary schools in Kastamonu and 3rd and 4th grade pre-service teachers who study at the Science Teaching program of Faculty of Education Kastamonu University and to examine how change according to some variables (education level, seniority year, gender, parents’ educational levels, etc…). For this purpose, Universal Science Literacy Scale (USLS) has been applied to 109 science teachers and 142 science pre-service teachers. This research using quantitative research design is in the survey model. SPSS package program has been used to analyze the data obtained from the research. As a result of the analysis of the data, it has been determined that the universal science literacy level of science teachers is “very high” and that of science pre-service teachers is “high”. Also, it has been determined that the education level of teachers, seniority years and gender variable have no affect the universal science literacy scores, and the gender variable has not effective in science pre-service teachers as it was in teachers. In addition, it has been observed that the parents’ educational level, the type of high schools they graduated from where, grade level and the environment in which they completed their education have no effect on the universal science literacy scores of the science preservice teachers. In order to increase the level of universal science literacy, it is important to make students love science at every stage from pre-school to university, to explain the subjects by associating them with daily life and to support them with experiments when necessary.