Effect of Hemolysis on Beckman Coulter High Sensitive Cardiac Troponin I Measurement

ilanbey, Bilal; GÜÇLÜ, KENAN

Effect of Hemolysis on Beckman Coulter High Sensitive Cardiac Troponin I Measurement

Bilal ILANBEY, (Kırşehir Ahi Evran Üniversitesi, Tıp Fakültesi, Tıbbi Biyokimya, Kırşehir, Türkiye)

Kenan GUCLU (Kırşehir Eğitim ve Araştırma Hastanesi, Biyokimya Laboratuvarı, Kırşehir, Türkiye)

Türk Klinik Biyokimya Dergisi

26 0

Yıl: 2021 Cilt: 19 Sayı: 2 Sayfa Aralığı: 139 - 144 Metin Dili: İngilizce İndeks Tarihi: 18-11-2022

Effect of Hemolysis on Beckman Coulter High Sensitive Cardiac Troponin I Measurement

Öz:

Aim: Measurement of cardiac troponins is of great importance in the diagnosis and follow-up of acute myocardial infarction. The diagnostic specificity of myocardial infarction has increased with algorithms including serial troponin measurements. It is important that the measurements in the troponin cut-off values used to evaluate the risk of myocardial infarction in algorithms are reliable. In this study, it was aimed to evaluate the effect of hemolysis on clinical decision concentrations of the high sensitive cardiac troponin I (hs cTnI) test. Material and Methods: Two pools were created from serum samples with the 99th percentile upper reference value (17.5 ng/L) of hs cTnI and the high-risk cut-off value (50 ng/L) as specified in the European Society of Cardiology 2020 manual. Hemolysate was spiked in both pools with a hemolysis index of 100 to 1000. The hs cTnI levels in hemolysate-added sera were measured in Beckman Coulter UniCel Dxl 800 immunoassay device. Levels of hemolysis were calculated as percentage change. Results: Due to hemolysis, hs cTnI values decreased in varying proportions from 3.8% to 20.4% at 99th percentile concentration; values decreased from 4.3% to 20.0% in high-risk cut-off concentration. Conclusions: In hemolyzed samples, hs cTnI values decrease, this decrease is not considered clinically significant until high hemolysis values. However, it should be kept in mind that a decrease that may occur even in a low hemolysis may lead to erroneous evaluations in clinical decision concentrations.

Anahtar Kelime:

Beckman Coulter Yüksek Duyarlıklı Kardiyak Troponin I Ölçümü Üzerine Hemolizin Etkisi

Öz:

Amaç: Kardiyak troponinlerin ölçümü akut miyokard enfarktüsünün tan ı ve takibinde büyük önem taşımaktadırlar. Seri troponin ölçümlerinin yer ald ığı algoritmalar ile miyokard enfarktüs tan ısının spesifisitesi artmıştır. Algoritmalarda, miyokard enfarktüs riskini değerlendirmede yer alan troponin cut- off de ğerlerindeki ölçümlerin güvenilir olmas ı önemlidir. Bu çal ışmada, yüksek duyarl ıklı kardiyak troponin I (hs cTnI) testinin klinik karar konsantrasyonları üzerine hemolizin etkisini de ğerlendirmek amaçlandı. Gereç ve Yöntem: Serum örneklerinden, hs cTnI’nin 99. persentil üst referans değeri (17.5 ng/L) ve Avrupa Kardiyoloji Derne ği 2020 k ılavuzunda belirtilen yüksek-risk cut-off de ğeri (50 ng/L) olacak şekilde iki havuz olu şturuldu. Her iki havuza hemoliz indeksi 100’den 1000’e kadar olacak şekilde hemolizat eklendi. Hemolizat eklenmi ş serumlarda hs cTnI düzeyleri Beckman Coulter UniCel Dxl 800 immünoassay cihazında ölçüldü. Hemolizden etkilenme düzeyleri yüzde değişim olarak hesaplandı. Bulgular: Hemoliz nedeniyle hs cTnI değerleri, 99. persentil konsantrasyonunda %3.8’den %20.4’e; yüksek-risk cut-off konsantrasyonunda %4.3’ten %20’ye kadar değişen oranlarda azaldı. Sonuç: Hemolize örneklerde hs cTnI değerleri azal ır, bu azalma yüksek hemoliz de ğerlerine kadar klinik olarak anlamlı kabul edilmemektedir. Ancak düşük bir hemolizde bile olu şabilecek bir azalman ın klinik karar konsantrasyonlarında hatalı değerlendirmelere yol açabileceği unutulmamalıdır.

Anahtar Kelime:

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	ilanbey B, GÜÇLÜ K (2021). Effect of Hemolysis on Beckman Coulter High Sensitive Cardiac Troponin I Measurement. , 139 - 144.
Chicago	ilanbey Bilal,GÜÇLÜ KENAN Effect of Hemolysis on Beckman Coulter High Sensitive Cardiac Troponin I Measurement. (2021): 139 - 144.
MLA	ilanbey Bilal,GÜÇLÜ KENAN Effect of Hemolysis on Beckman Coulter High Sensitive Cardiac Troponin I Measurement. , 2021, ss.139 - 144.
AMA	ilanbey B,GÜÇLÜ K Effect of Hemolysis on Beckman Coulter High Sensitive Cardiac Troponin I Measurement. . 2021; 139 - 144.
Vancouver	ilanbey B,GÜÇLÜ K Effect of Hemolysis on Beckman Coulter High Sensitive Cardiac Troponin I Measurement. . 2021; 139 - 144.
IEEE	ilanbey B,GÜÇLÜ K "Effect of Hemolysis on Beckman Coulter High Sensitive Cardiac Troponin I Measurement." , ss.139 - 144, 2021.
ISNAD	ilanbey, Bilal - GÜÇLÜ, KENAN. "Effect of Hemolysis on Beckman Coulter High Sensitive Cardiac Troponin I Measurement". (2021), 139-144.

APA	ilanbey B, GÜÇLÜ K (2021). Effect of Hemolysis on Beckman Coulter High Sensitive Cardiac Troponin I Measurement. Türk Klinik Biyokimya Dergisi, 19(2), 139 - 144.
Chicago	ilanbey Bilal,GÜÇLÜ KENAN Effect of Hemolysis on Beckman Coulter High Sensitive Cardiac Troponin I Measurement. Türk Klinik Biyokimya Dergisi 19, no.2 (2021): 139 - 144.
MLA	ilanbey Bilal,GÜÇLÜ KENAN Effect of Hemolysis on Beckman Coulter High Sensitive Cardiac Troponin I Measurement. Türk Klinik Biyokimya Dergisi, vol.19, no.2, 2021, ss.139 - 144.
AMA	ilanbey B,GÜÇLÜ K Effect of Hemolysis on Beckman Coulter High Sensitive Cardiac Troponin I Measurement. Türk Klinik Biyokimya Dergisi. 2021; 19(2): 139 - 144.
Vancouver	ilanbey B,GÜÇLÜ K Effect of Hemolysis on Beckman Coulter High Sensitive Cardiac Troponin I Measurement. Türk Klinik Biyokimya Dergisi. 2021; 19(2): 139 - 144.
IEEE	ilanbey B,GÜÇLÜ K "Effect of Hemolysis on Beckman Coulter High Sensitive Cardiac Troponin I Measurement." Türk Klinik Biyokimya Dergisi, 19, ss.139 - 144, 2021.
ISNAD	ilanbey, Bilal - GÜÇLÜ, KENAN. "Effect of Hemolysis on Beckman Coulter High Sensitive Cardiac Troponin I Measurement". Türk Klinik Biyokimya Dergisi 19/2 (2021), 139-144.