Objective: Although hemoglobin, folate, 25 (OH) vitamin D3, and vitamin B12 levels have been reported to be associated with the risk of stroke, there are limited data on the relationship between these levels and functional sta tus, disability, pain or balance in those who develop post-stroke hemiplegia. The present study aimed to investigate the relationship between parameters such as functional status, pain, balance, and hemoglobin, folate, 25 (OH) vitamin D3, and vitamin B12 levels in patients with post-stroke hemiplegia. Material and Meth ods: Thirty-nine patients were included in the study. Brunnstrom recovery stages, Berg Balance Scale, Functional Ambulation Category (FAC), Leeds Assessment of Neuropathic Symptoms and Signs pain scale, Barthel Index scores were de termined. In addition, the patients’ central pain and hemiplegic shoulder pain were evaluated using numerical rating scales. The hemoglobin, folate, vitamin B12, and 25 (OH) vitamin D3 levels of the participants were noted. Results: Hemoglobin levels were significantly higher in functional ambulatory patients (FAC Stage 3-5) than in non-functional ambulatory (FAC Stage 0-2) patients (mean±standard deviation 13.77±2.38, 12.13±1.58, respectively) (p=0.019). In ad dition, hemoglobin levels were positively correlated with Barthel Index (r=0.496, p=0.002) and Berg Balance Scale (r=0.458, p=0.005). Folate, vitamin B12, and 25 (OH) vitamin D3 levels were not associated with any of the scales. According to the results of the multivariate logistic regression analysis, the increase in the hemoglobin values of the patients increases the probability of being in the functional ambulatory group 1,655 times (p=0.024). Conclusion: In this study, hemoglobin level was found to be associated with disability, ambulation and balance status in patients with post-stroke hemiplegia. For better rehabilitation success, it should be kept in mind to evaluate the hemoglobin levels of the patients.