Gossypin Suppresses Cell Growth by Cytotoxic Effect and Induces Apoptosis in MCF-7 Cells
Yıl: 2022 Cilt: 4 Sayı: 1 Sayfa Aralığı: 21 - 26 Metin Dili: İngilizce DOI: 10.37990/medr.996944 İndeks Tarihi: 27-09-2022
Gossypin Suppresses Cell Growth by Cytotoxic Effect and Induces Apoptosis in MCF-7 Cells
Öz: Aim: Today, breast cancer is a disease that is encountered commonly in women with limited options for treatment. It is needed to find new agents that can be effective in preventing or managing this disease. It has been demonstrated that gossypin inhibits tumor growth. In our study, it has been targeted to examine the effects of gossypin regarding both anticancer activity and apoptosis in MCF-7 cells. Material and Method: MCF-7 cells were treated with different doses of gossypin and with 50 µM cisplatin for 24, 48, and 72 hours. The MTT analysis, Caspase-3, Caspase-9, and Nf-KB mRNA expressions of those MCF-7 cells which were treated with gossypin were also conducted in order to evaluate the apoptosis or necroptosis-induced cell death. Results: In MTT experiments, it has been observed that the administration of 100 µM dose of gossypin had similar effects to the routine cisplatin administration, caused a significant decrease in cell proliferation, and increased apoptosis in the evaluations of Hoechst staining and morphology. It has been put forth that gossypin decreases the expression of CASP-3 and CASP-9 mRNA and increases the expression of NF-kB.Conclusion: Our results demonstrate that for the breast cancer cells, the 100 µM of gossypin positively affects cell death pathways due to apoptosis.
Anahtar Kelime: Gossypin, Sitotoksik Etki ile Hücre Büyümesini Baskılar ve MCF-7 Hücrelerinde Apoptozu İndüklemektedir
Öz: Amaç: Meme kanseri günümüzde kadınlarda sık görülen ve tedavi seçenekleri kısıtlı olan bir hastalıktır. Bu hastalığı önlemede veya yönetmede etkili olabilecek yeni ajanların bulunması gerekmektedir. Gossypin’in tümör büyümesini engellediği gösterilmiştir. Çalışmamızda MCF-7 hücrelerinde hem antikanser aktivitesi hem de apoptoz üzerine gossypin’in etkilerinin incelenmesi hedeflenmiştir. Materyal ve Metot: MCF-7 hücreleri, 24, 48 ve 72 saat boyunca farklı dozlarda gossipin ve 50 uM sisplatin ile muamele edildi. Apoptoz veya nekroptoz kaynaklı hücre ölümünü değerlendirmek için, gossipin ile tedavi edilen MCF-7 hücrelerinin MTT analizi, Caspase-3, Caspase-9 ve Nf-KB mRNA ekspresyonları da yapıldı. Bulgular: MTT deneylerinde 100 μM dozda gossypin uygulamasının rutin sisplatin uygulamasına benzer etkiler gösterdiği, Hoechst boyaması ve morfolojisi değerlendirmelerinde hücre proliferasyonunda anlamlı azalmaya ve apoptoz artışına neden olduğu gözlendi. Gossypin’in CASP-3 ve CASP-9 mRNA ekspresyonunu azalttığı ve NF-kB ekspresyonunu arttırdığı ileri sürülmüştür. Sonuç: Sonuçlarımız meme kanseri hücreleri için 100 μM gossipinin apoptoza bağlı hücre ölüm yollarını olumlu etkilediğini göstermektedir.
Anahtar Kelime: Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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| APA | Cicek M, ÇINAR İ, AKSAK KARAMESE S (2022). Gossypin Suppresses Cell Growth by Cytotoxic Effect and Induces Apoptosis in MCF-7 Cells. , 21 - 26. 10.37990/medr.996944 |
| Chicago | Cicek Mustafa,ÇINAR İRFAN,AKSAK KARAMESE Selina Gossypin Suppresses Cell Growth by Cytotoxic Effect and Induces Apoptosis in MCF-7 Cells. (2022): 21 - 26. 10.37990/medr.996944 |
| MLA | Cicek Mustafa,ÇINAR İRFAN,AKSAK KARAMESE Selina Gossypin Suppresses Cell Growth by Cytotoxic Effect and Induces Apoptosis in MCF-7 Cells. , 2022, ss.21 - 26. 10.37990/medr.996944 |
| AMA | Cicek M,ÇINAR İ,AKSAK KARAMESE S Gossypin Suppresses Cell Growth by Cytotoxic Effect and Induces Apoptosis in MCF-7 Cells. . 2022; 21 - 26. 10.37990/medr.996944 |
| Vancouver | Cicek M,ÇINAR İ,AKSAK KARAMESE S Gossypin Suppresses Cell Growth by Cytotoxic Effect and Induces Apoptosis in MCF-7 Cells. . 2022; 21 - 26. 10.37990/medr.996944 |
| IEEE | Cicek M,ÇINAR İ,AKSAK KARAMESE S "Gossypin Suppresses Cell Growth by Cytotoxic Effect and Induces Apoptosis in MCF-7 Cells." , ss.21 - 26, 2022. 10.37990/medr.996944 |
| ISNAD | Cicek, Mustafa vd. "Gossypin Suppresses Cell Growth by Cytotoxic Effect and Induces Apoptosis in MCF-7 Cells". (2022), 21-26. https://doi.org/10.37990/medr.996944 |
| APA | Cicek M, ÇINAR İ, AKSAK KARAMESE S (2022). Gossypin Suppresses Cell Growth by Cytotoxic Effect and Induces Apoptosis in MCF-7 Cells. Medical records-international medical journal (Online), 4(1), 21 - 26. 10.37990/medr.996944 |
| Chicago | Cicek Mustafa,ÇINAR İRFAN,AKSAK KARAMESE Selina Gossypin Suppresses Cell Growth by Cytotoxic Effect and Induces Apoptosis in MCF-7 Cells. Medical records-international medical journal (Online) 4, no.1 (2022): 21 - 26. 10.37990/medr.996944 |
| MLA | Cicek Mustafa,ÇINAR İRFAN,AKSAK KARAMESE Selina Gossypin Suppresses Cell Growth by Cytotoxic Effect and Induces Apoptosis in MCF-7 Cells. Medical records-international medical journal (Online), vol.4, no.1, 2022, ss.21 - 26. 10.37990/medr.996944 |
| AMA | Cicek M,ÇINAR İ,AKSAK KARAMESE S Gossypin Suppresses Cell Growth by Cytotoxic Effect and Induces Apoptosis in MCF-7 Cells. Medical records-international medical journal (Online). 2022; 4(1): 21 - 26. 10.37990/medr.996944 |
| Vancouver | Cicek M,ÇINAR İ,AKSAK KARAMESE S Gossypin Suppresses Cell Growth by Cytotoxic Effect and Induces Apoptosis in MCF-7 Cells. Medical records-international medical journal (Online). 2022; 4(1): 21 - 26. 10.37990/medr.996944 |
| IEEE | Cicek M,ÇINAR İ,AKSAK KARAMESE S "Gossypin Suppresses Cell Growth by Cytotoxic Effect and Induces Apoptosis in MCF-7 Cells." Medical records-international medical journal (Online), 4, ss.21 - 26, 2022. 10.37990/medr.996944 |
| ISNAD | Cicek, Mustafa vd. "Gossypin Suppresses Cell Growth by Cytotoxic Effect and Induces Apoptosis in MCF-7 Cells". Medical records-international medical journal (Online) 4/1 (2022), 21-26. https://doi.org/10.37990/medr.996944 |
