Expression of DNA methyltransferases (DNMTs) at mRNA level in ovine endometrium during estrus cycle and early pregnancy
Yıl: 2022 Cilt: 38 Sayı: 3 Sayfa Aralığı: 143 - 149 Metin Dili: İngilizce DOI: 10.15312/EurasianJVetSci.2022.376 İndeks Tarihi: 22-10-2022
Expression of DNA methyltransferases (DNMTs) at mRNA level in ovine endometrium during estrus cycle and early pregnancy
Öz: Aim: To elucidate mRNA expression of DNA methyltransferase enzyme genes (DNMT1, DNMT3A, and DNMT3B) in ovine endometrium whether they are modulated during the estrus cycle and early pregnancy. Materials and Methods: The endometrial samples including intercaruncular sites was obtained from a total of 24 ewes on days of 12 (P12, n = 4), 16 (P16, n = 4) and 22 (P22, n = 4) of pregnancy following mating and cyclic days of 12 (C12, n = 4), 16 (C16, n = 4) and 22 (C22, n = 4) of the estrous cycle. The relative mRNA level of DNMTs were evaluated through real-time quantitative RT-qPCR. Results: Abundances of DNMTs including DNMT1, DNMT3A, and DNMT3B were detected during the estrous cycle and early pregnancy in the ovine endometrium by this study. DNMT1 mRNA steady-state level was greater in P16 than in C16 whereas they did not change within the rest of the groups. The level of DNMT3A mRNA, during early pregnancy, had the highest expression levels on P12 compared to P22 and P16 (p<0.01). However, the level of DNMT3A mRNA was lower in P16 than in C16 (p<0.01) and had a similar decrease in P22 compared to C22 (p<0.01). DNMT3B mRNA level did not differ (p>0.01) during the estrus cycle and early pregnancy. Conclusion: DNA methylation related DNMTs may be required to control of gene expression in the ovine endometrium during the estrus cycle and early pregnancy.
Anahtar Kelime: Östrus siklusu ve erken gebelikte koyun endometriyumunda DNA metiltransferazların (DNMT) mRNA düzeyinde ekspresyonu
Öz: Amaç: Bu çalışmanın amacı, östrus döngüsü ve erken gebelik sırasında DNA metiltransferaz enzim genlerinin (DNMT1, DNMT3A ve DNMT3B) ekspresyonunun koyun endometriyumunda düzenlenip düzenlenmediğini açıklamaktır. Gereç ve Yöntem: İnterkarunkular bölgeleri içeren endometriyal örnekler çiftleşmeyi takiben gebeliğin 12 (P12, n = 4), 16 (P16, n = 4) ve 22. (P22, n = 4) günlerinde ve östrus döngüsününe karşılık gelen 12. (C12, n = 4), 16. (C16, n = 4) ve 22. (C22, n = 4) günlerde toplam 24 koyundan toplandı. DNMT'lerin nispi mRNA ekspresyon düzeyleri gerçek zamanlı polimeraz zincir reaksiyonu (RT-qPCR) kullanılarak değerlendirildi. Bulgular: Tüm DNMT'lerin ekspresyonları östrus döngüsü ve erken gebelik sırasında koyun endometriyumda tespit edildi. DNMT1 mRNA ekspresyonunun kararlı durum seviyeleri P16'da C16'dan daha yüksekti, ancak grupların geri kalanında değişmedi. Bununla birlikte, gebeliğin erken döneminde DNMT3A mRNA seviyesi P22 ve P16'ya kıyasla P12'de en yüksek ekspresyon seviyelerine sahipti (p<0.01). Öte yandan, DNMT3A mRNA seviyesi P16'da C16'dandaha düşüktü (p<0.01), ayrıca P22'de C22'ye kıyasla benzer bir düşüş bulundu (p<0.01). DNMT3B mRNA ekspresyon seviyesi ise hem östrus döngüsü ve hemde erken gebelik sırasında değişmedi. Öneri: Östrus siklusu ve erken gebelik sırasında koyun endometriyumundaki gen ekspresyonunun kontrolü için DNA metilasyonu ile ilgili DNMT'ler gerekli olabileceği kanısına varıldı.
Anahtar Kelime: Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
- Atli MO, Guzeloglu A, Dinc DA, 2011. Expression of wingless type (WNT) genes and their antagonists at mRNA levels in equine endometrium during the estrous cycle and early pregnancy. Anim Reprod Sci, 125, 1-4, 94-102.
- Atli MO, Kayış SA, Güzeloğlu A, Aslan S, et al., 2012. Expression of epidermal growth factor (EGF) and heparinbinding EGF (HB-EGF) mRNA in mare endometrium. Kafkas Üniversitesi Vet Fakültesi Derg, 18, 463–7.
- Atli MO, Kurar E, Kayis SA, Aslan S, et al., 2010. Evaluation of genes involved in prostaglandin action in equine endometrium during estrous cycle and early pregnancy. Anim Reprod Sci, 122, 1-2, 124-32.
- Bindea G, Mlecnik B, Hackl H, Charoentong P, et al., 2009. ClueGO: a Cytoscape plug-in to decipher functionally grouped gene ontology and pathway annotation networks. Bioinformatics, 258, 1091-1093.
- Brooks K, Burns GW, Moraes JG, Spencer TE, 2016. Analysis of the uterine epithelial and conceptus transcriptome and luminal fluid proteome during the peri-implantation period of pregnancy in sheep. Biol Reprod, 95, 4, 88-1.
- Caplakova V, Babusikova E, Blahovcova E, Balharek T, et al., 2016. DNA methylation machinery in the endometrium and endometrial cancer. Anticancer Res. 36, 9, 4407-20.
- Chen K, Chen X, He J, Ding Y, et al., 2015. Mouse endometrium temporal and spatial expression mRNA and microRNA associated with embryo implantation. Reprod Sci, 22, 11, 1399-408.
- Curradi M, Izzo A, Badaracco G, Landsberger N, 2002. Molecular mechanisms of gene silencing mediated by DNA methylation. Mol Cell Biol, 22, 9, 3157-73.
- Ding YB, He JL, Liu XQ, Chen XM, et al., 2012. Expression of DNA methyltransferases in the mouse uterus during early pregnancy and susceptibility to dietary folate deficiency. Reproduction, 144, 91.
- Fürst RW, Meyer HH, Schweizer G, Ulbrich SE, 2012. Is DNA methylation an epigenetic contribution to transcriptional regulation of the bovine endometrium during the estrous cycle and early pregnancy? Mol Cell Endocrino, 348, 67-77.
- Gagliardi M, Strazzullo M, Matarazzo MR, 2018. DNMT3B functions: novel insights from human disease. Front Cell Dev Biol. 6, 140.
- Gao F, Ma X, Rusie A, Hemingway J, et al., 2012. Epigenetic changes through DNA methylation contribute to uterine stromal cell decidualization. Endocrinology, 153, 12, 6078-6090.
- Hitit M, Kurar E, Güzeloğlu A, 2015a. MikroRNA biyogenezi. Ataturk Univ Vet Bilim Derg, 18, 10(3). Hitit M, Atli M, Ozel C, Guzeloglu A, et al., 2015b. Expression of DNA methyltransferases (DNMTs) at mRNA level in equine endometrium: P57. Reprod Domest Anim, 50, 56–7.
- Hitit M, Corum O, Corum DD, Donmez H, et al., 2018. A cardioprotective role of Nerium oleander with the expression of hypoxia inducible factor 2A mRNA by increasing antioxidant enzymes in rat heart tissue. Acta Sci Vet, 46, 8.
- Hitit M, Corum O, Ozbek M, Uney K, et al., 2020. Mucus from different fish species alleviates carrageenan-induced inflammatory paw edema in rats. Asian Pac J Trop Biomed, 10, 452.
- Iqbal K, Jin SG, Pfeifer GP, Szabó PE, 2011. Reprogramming of the paternal genome upon fertilization involves genome wide oxidation of 5-methylcytosine. Proc Natl Acad Sci, 108, 9, 3642-7.
- Kadayifci FZ, Zheng S, Pan YX, 2018. Molecular mechanisms underlying the link between diet and DNA methylation. Int J Mol Sci, 19, 12, 4055.
- Law PP, Holland ML, 2019. DNA methylation at the crossroads of gene and environment interactions. Essays Biochem, 63, 6, 717-726.
- Li N, Lu S, Ding Y, Chen X, et al., 2020. Uterine Dnmt3a is not required for mouse embryo implantation. Curr Mol Med, 20, 8, 633-642.
- Livak KJ, Schmittgen TD, 2001. Analysis of relative gene expression data using real-time quantitative PCR and the 2-ΔΔCT method. Methods, 25, 4, 402-8.
- Long J, Li W, Chen M, Ding Y, et al., 2021. Uterine deficiency of Dnmt3b impairs decidualization and causes consequent embryo implantation defects. Cell Biol Toxicol, 1-22.
- Mahajan V, Osavlyuk D, Logan PC, Amirapu S, et al., 2020. Expression and steroid hormone regulation of TETs and DNMTs in human endometrium. Reproduction, 160, 2, 247-257.
- Mazzoni G, Pedersen HS, Rabaglino MB, Hyttel P, et al., 2020. Characterization of the endometrial transcriptome in early diestrus influencing pregnancy status in dairy cattle after transfer of in vitro-produced embryos. Physiol Genomics, 52, 7, 269-79.
- Munro SK, Farquhar CM, Mitchell MD, Ponnampalam AP, 2010. Epigenetic regulation of endometrium during the menstrual cycle. Mol Hum Reprod, 16, 5, 297-310. Phillips T, 2008. The role of methylation in gene expression. Nat. Sci. Educ, 1, 116
- Ponsuksili S, Murani E, Schwerin M, Schellander K, et al., 2012. Gene expression and DNA-methylation of bovine pretransfer endometrium depending on its receptivity after in vitro-produced embryo transfer. PLoS One. 7, 8:e42402.
- Salilew-Wondim D, Schellander K, Hoelker M, Tesfaye D. et al., 2012. Oviductal, endometrial and embryonic gene expression patterns as molecular clues for pregnancy establishment. Anim Reprod Sci, 134, 1-2, 9-18.
- Shimizu T, Krebs S, Bauersachs S, Blum H, et al., 2010. Actions and interactions of progesterone and estrogen on transcriptome profiles of the bovine endometrium. Physiol Genomics, 42, 4, 290-300.
- Spencer TE, Johnson GA, Bazer FW, Burghardt RC., 2004. Implantation mechanisms: insights from the sheep. Reproduction, 128, 657–68.
- Talbi S, Hamilton AE, Vo KC, Tulac S, et al., 2006. Molecular phenotyping of human endometrium distinguishes menstrual cycle phases and underlying biological processes in normo-ovulatory women. Endocrinology, 147, 3, 1097-1121.
- Tavalaee M, Razavi S, Nasr-Esfahani MH, 2009. Influence of sperm chromatin anomalies on assisted reproductive technology outcome. Fertil Steril, 91, 4, 1119-26.
- Vavouri T, Lehner B, 2011. Chromatin organization in sperm may be the major functional consequence of base composition variation in the human genome. PLoS Genetics, 7, 4, e1002036.
- Vincent ZL, Farquhar CM, Mitchell MD, Ponnampalam, AP, 2011.ExpressionandregulationofDNAmethyltransferases in human endometrium. Fertil Steril, 95, 4, 1522-1525.
- Wang X, Li B, 2017. DNMT1 regulates human endometrial carcinoma cell proliferation. Onco. Targets. Ther, 10, 1865.
- Yang H, Fu L, Luo Q, Li L, et al., 2021. Comparative analysis of differentially expressed miRNAs related to uterine involution in the ovine ovary and uterus. Arch Anim Breed, 64, 167-175.
- Zelenko Z, Aghajanova L, Irwin JC, Giudice LC, 2012. Nuclear receptor, coregulator signaling, and chromatin remodeling pathways suggest involvement of the epigenome in the steroid hormone response of endometrium and abnormalities in endometriosis. Reprod Sci, 19, 2, 152-62.
| APA | Hitit M, KOSE M (2022). Expression of DNA methyltransferases (DNMTs) at mRNA level in ovine endometrium during estrus cycle and early pregnancy. , 143 - 149. 10.15312/EurasianJVetSci.2022.376 |
| Chicago | Hitit Mustafa,KOSE MEHMET Expression of DNA methyltransferases (DNMTs) at mRNA level in ovine endometrium during estrus cycle and early pregnancy. (2022): 143 - 149. 10.15312/EurasianJVetSci.2022.376 |
| MLA | Hitit Mustafa,KOSE MEHMET Expression of DNA methyltransferases (DNMTs) at mRNA level in ovine endometrium during estrus cycle and early pregnancy. , 2022, ss.143 - 149. 10.15312/EurasianJVetSci.2022.376 |
| AMA | Hitit M,KOSE M Expression of DNA methyltransferases (DNMTs) at mRNA level in ovine endometrium during estrus cycle and early pregnancy. . 2022; 143 - 149. 10.15312/EurasianJVetSci.2022.376 |
| Vancouver | Hitit M,KOSE M Expression of DNA methyltransferases (DNMTs) at mRNA level in ovine endometrium during estrus cycle and early pregnancy. . 2022; 143 - 149. 10.15312/EurasianJVetSci.2022.376 |
| IEEE | Hitit M,KOSE M "Expression of DNA methyltransferases (DNMTs) at mRNA level in ovine endometrium during estrus cycle and early pregnancy." , ss.143 - 149, 2022. 10.15312/EurasianJVetSci.2022.376 |
| ISNAD | Hitit, Mustafa - KOSE, MEHMET. "Expression of DNA methyltransferases (DNMTs) at mRNA level in ovine endometrium during estrus cycle and early pregnancy". (2022), 143-149. https://doi.org/10.15312/EurasianJVetSci.2022.376 |
| APA | Hitit M, KOSE M (2022). Expression of DNA methyltransferases (DNMTs) at mRNA level in ovine endometrium during estrus cycle and early pregnancy. Eurasian Journal of Veterinary Sciences, 38(3), 143 - 149. 10.15312/EurasianJVetSci.2022.376 |
| Chicago | Hitit Mustafa,KOSE MEHMET Expression of DNA methyltransferases (DNMTs) at mRNA level in ovine endometrium during estrus cycle and early pregnancy. Eurasian Journal of Veterinary Sciences 38, no.3 (2022): 143 - 149. 10.15312/EurasianJVetSci.2022.376 |
| MLA | Hitit Mustafa,KOSE MEHMET Expression of DNA methyltransferases (DNMTs) at mRNA level in ovine endometrium during estrus cycle and early pregnancy. Eurasian Journal of Veterinary Sciences, vol.38, no.3, 2022, ss.143 - 149. 10.15312/EurasianJVetSci.2022.376 |
| AMA | Hitit M,KOSE M Expression of DNA methyltransferases (DNMTs) at mRNA level in ovine endometrium during estrus cycle and early pregnancy. Eurasian Journal of Veterinary Sciences. 2022; 38(3): 143 - 149. 10.15312/EurasianJVetSci.2022.376 |
| Vancouver | Hitit M,KOSE M Expression of DNA methyltransferases (DNMTs) at mRNA level in ovine endometrium during estrus cycle and early pregnancy. Eurasian Journal of Veterinary Sciences. 2022; 38(3): 143 - 149. 10.15312/EurasianJVetSci.2022.376 |
| IEEE | Hitit M,KOSE M "Expression of DNA methyltransferases (DNMTs) at mRNA level in ovine endometrium during estrus cycle and early pregnancy." Eurasian Journal of Veterinary Sciences, 38, ss.143 - 149, 2022. 10.15312/EurasianJVetSci.2022.376 |
| ISNAD | Hitit, Mustafa - KOSE, MEHMET. "Expression of DNA methyltransferases (DNMTs) at mRNA level in ovine endometrium during estrus cycle and early pregnancy". Eurasian Journal of Veterinary Sciences 38/3 (2022), 143-149. https://doi.org/10.15312/EurasianJVetSci.2022.376 |
