Antimicrobial and Antioxidant Potential of Silver Nanoparticles Synthesized from Primula vulgaris

Belgin ERDEM, (Kırşehir Ahi Evran Üniversitesi, Sağlık Hizmetleri Meslek Yüksekokulu, Kırşehir, Türkiye)
Harun ÇİFTÇİ, (Kırşehir Ahi Evran Üniversitesi, Tıp Fakültesi, Tıbbi Biyokimya Anabilim Dalı, Kırşehir, Türkiye)
Yunus Emre ŞAHİN (Kırşehir Ahi Evran Üniversitesi, Doğa Bilimleri Enstitüsü, Kırşehir, Türkiye)
Muş Alparslan Üniversitesi Fen Bilimler Dergisi
9 0
Yıl: 2022 Cilt: 10 Sayı: 2 Sayfa Aralığı: 1013 - 1022 Metin Dili: İngilizce DOI: 10.18586/msufbd.1126542 İndeks Tarihi: 21-02-2023

Antimicrobial and Antioxidant Potential of Silver Nanoparticles Synthesized from Primula vulgaris

Öz:
In this study, biosynthesis and in vitro phytochemical composition, antibacterial and antioxidant activities of silver nanoparticles were investigated by using aqueous leaf, flower and root extracts of Primula vulgaris (P. vulgaris subsp. vulgaris). The synthesized silver nanoparticles (AgNPs) were confirmed by color conversion and ultraviolet-visible (UV-visible) spectrophotometry. The appearance of a dark brown color and a UV absorption spectrum range at 440 nm confirmed the synthesized silver nanoparticles. The antimicrobial activity of silver nanoparticles synthesized from the leaf of P. vulgaris; S. aureus 25±1, S. epidermidis 20±1, P. aeruginosa 20±1, A. hydrophila 21±1, C. albicans 25±1, C. tropicalis 25±1, C. parapsilosis 22±1 and C. glabrata 20±1 mm zone diameter was determined. The most antimicrobial effect of P. vulgaris leaf aqueous extract; S. aureus 20±1, S. epidermidis 18±1, A.hydrophila 15±1, P. aeruginosa 12±2, C. albicans 18±1, C. glabrata 18±1, C.tropicalis15±2, and C. parapsilosis 15±2 mm zone diameter was revealed. The presence of flavonoids, terpenoids, protein, and carbohydrates was found to be higher in silver nanoparticles synthesized in the flower part of P. vulgaris, according to phytochemical screening. While saponins were detected in P. vulgaris root extracts, tannins and protein were detected in the leaf extract. The flower had the highest total phenolic extract content of the silver nanoparticle (29.08±0 mg GAE/g DW), while the leaf and root had the lowest total phenolic content of 9.06±0.5 and 8.64±3.3 mg GAE/g DW, respectively. The flower had the highest total phenolic extract content of the plant aqueous extracts (25.10±0.2 mg GAE/g DW), while the leaf and root had the lowest (8.28±0.5 and 5.20±0.0 mg GAE/g DW, respectively).The DPPH (1,1-Diphenyl-2-picrylhydrazil) assay was used to assess free radical scavenging activity. The antioxidant activity of AgNPs biosynthesized using P. vulgaris flower extract was 90.6 %, while P. vulgaris flower aqueous extracts were 86.3 %. This can be concluded that silver nanoparticles synthesized using P. vulgaris flower extract are useful in the preparation of pharmacologically useful drugs.
Anahtar Kelime:

Primula vulgaris'ten Sentezlenen Gümüş Nanopartiküllerin Antimikrobiyal ve Antioksidan Potansiyeli

Öz:
Bu çalışmada, Primula vulgaris'in (P. vulgaris subsp. vulgaris) sulu yaprak, çiçek ve kök ekstreleri kullanılarak gümüş nanopartiküllerin biyosentezi ve in vitro fitokimyasal bileşimi, antibakteriyel ve antioksidan aktiviteleri araştırılmıştır. Sentezlenen gümüş nanoparçacıklar (AgNP'ler), renk dönüşümü ve ultraviyole-görünür (UV-görünür) spektrofotometrisi ile doğrulandı. Koyu kahverengi rengin görünümü ve 440 nm'de bir UV absorpsiyon spektrum aralığı, sentezlenen gümüş nanoparçacıkları doğruladı. P. vulgaris’in yaprak ekstraktından sentezlenen gümüş nanopartiküldeki en fazla antimikrobiyal etki; S. aureus 25±1, S. epidermidis 20±1, P. aeruginosa 21±1, A. hydrophila 21±1, C. albicans 25±1, C. tropicalis 25±1, C. parapsilosis 22±1 ve C.glabrata 20±1 mm zon çapı ile belirlendi.P. vulgaris yaprak sulu ekstraktın en fazla antimikrobiyal etki; S. aureus 20±1, S. epidermidis 18±1, A.hydrophila 15±1, P. aeruginosa 12±2, C. albicans 18±1, C. glabrata 18±1, C.tropicalis15±2 ve C. parapsilosis 15±2 mm zon çapı ortaya konmuştur. Fitokimyasal taramaya göre P. vulgaris'in çiçek kısmında sentezlenen gümüş nanopartiküllerde flavonoidler, terpenoidler, protein ve karbonhidratların varlığı daha yüksek bulunmuştur. P. vulgaris kök ekstraktlarında saponinler tespit edilirken, yaprak ekstraktında tanen ve protein tespit edildi. P. vulgaris yaprak ekstraktından sentezlenen gümüş nanopartiküllerin antimikrobiyal aktivitesinin, P. vulgaris yaprak sulu ekstraktının antimikrobiyal aktivitesinden daha aktif olduğu bulundu. Çiçek, gümüş nanopartikülün en yüksek toplam fenolik ekstrakt içeriğine (29.08±0 mg GAE/g DW) sahipken, yaprak ve kök, sırasıyla 9.06±0.5 ve 8.64±3.3 mg GAE/g DW ile en düşük toplam fenolik içeriğe sahipti. Bitki sulu ekstraktları arasında en yüksek toplam fenolik ekstrakt içeriği çiçekte bulunurken (25.10±0.2 mg GAE/g DW), yaprak ve kök en düşük (sırasıyla 8.28±0.5 ve 5.20±0.0 mg GAE/g DW) bulundu. DPPH (1,1-Difenil-2- pikrilhidrazil) tahlili, serbest radikal süpürme aktivitesini değerlendirmek için kullanıldı. P. vulgaris çiçek özütü kullanılarak biyosentezlenen AgNP'lerin antioksidan aktivitesi %90,6 iken, P.vulgaris çiçek sulu özütleri %86.3'tür. Buradan P. vulgaris çiçek ekstresi kullanılarak sentezlenen gümüş nanopartiküllerin farmakolojik olarak faydalı ilaçların hazırlanmasında faydalı olduğu sonucuna varılabilir.
Anahtar Kelime:

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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APA Erdem B, Ciftci H, Şahin y (2022). Antimicrobial and Antioxidant Potential of Silver Nanoparticles Synthesized from Primula vulgaris. , 1013 - 1022. 10.18586/msufbd.1126542
Chicago Erdem Belgin,Ciftci Harun,Şahin yunus emre Antimicrobial and Antioxidant Potential of Silver Nanoparticles Synthesized from Primula vulgaris. (2022): 1013 - 1022. 10.18586/msufbd.1126542
MLA Erdem Belgin,Ciftci Harun,Şahin yunus emre Antimicrobial and Antioxidant Potential of Silver Nanoparticles Synthesized from Primula vulgaris. , 2022, ss.1013 - 1022. 10.18586/msufbd.1126542
AMA Erdem B,Ciftci H,Şahin y Antimicrobial and Antioxidant Potential of Silver Nanoparticles Synthesized from Primula vulgaris. . 2022; 1013 - 1022. 10.18586/msufbd.1126542
Vancouver Erdem B,Ciftci H,Şahin y Antimicrobial and Antioxidant Potential of Silver Nanoparticles Synthesized from Primula vulgaris. . 2022; 1013 - 1022. 10.18586/msufbd.1126542
IEEE Erdem B,Ciftci H,Şahin y "Antimicrobial and Antioxidant Potential of Silver Nanoparticles Synthesized from Primula vulgaris." , ss.1013 - 1022, 2022. 10.18586/msufbd.1126542
ISNAD Erdem, Belgin vd. "Antimicrobial and Antioxidant Potential of Silver Nanoparticles Synthesized from Primula vulgaris". (2022), 1013-1022. https://doi.org/10.18586/msufbd.1126542
APA Erdem B, Ciftci H, Şahin y (2022). Antimicrobial and Antioxidant Potential of Silver Nanoparticles Synthesized from Primula vulgaris. Muş Alparslan Üniversitesi Fen Bilimler Dergisi, 10(2), 1013 - 1022. 10.18586/msufbd.1126542
Chicago Erdem Belgin,Ciftci Harun,Şahin yunus emre Antimicrobial and Antioxidant Potential of Silver Nanoparticles Synthesized from Primula vulgaris. Muş Alparslan Üniversitesi Fen Bilimler Dergisi 10, no.2 (2022): 1013 - 1022. 10.18586/msufbd.1126542
MLA Erdem Belgin,Ciftci Harun,Şahin yunus emre Antimicrobial and Antioxidant Potential of Silver Nanoparticles Synthesized from Primula vulgaris. Muş Alparslan Üniversitesi Fen Bilimler Dergisi, vol.10, no.2, 2022, ss.1013 - 1022. 10.18586/msufbd.1126542
AMA Erdem B,Ciftci H,Şahin y Antimicrobial and Antioxidant Potential of Silver Nanoparticles Synthesized from Primula vulgaris. Muş Alparslan Üniversitesi Fen Bilimler Dergisi. 2022; 10(2): 1013 - 1022. 10.18586/msufbd.1126542
Vancouver Erdem B,Ciftci H,Şahin y Antimicrobial and Antioxidant Potential of Silver Nanoparticles Synthesized from Primula vulgaris. Muş Alparslan Üniversitesi Fen Bilimler Dergisi. 2022; 10(2): 1013 - 1022. 10.18586/msufbd.1126542
IEEE Erdem B,Ciftci H,Şahin y "Antimicrobial and Antioxidant Potential of Silver Nanoparticles Synthesized from Primula vulgaris." Muş Alparslan Üniversitesi Fen Bilimler Dergisi, 10, ss.1013 - 1022, 2022. 10.18586/msufbd.1126542
ISNAD Erdem, Belgin vd. "Antimicrobial and Antioxidant Potential of Silver Nanoparticles Synthesized from Primula vulgaris". Muş Alparslan Üniversitesi Fen Bilimler Dergisi 10/2 (2022), 1013-1022. https://doi.org/10.18586/msufbd.1126542
Sayfa Oluşturulma Tarihi
24-04-2024 5:3

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