Ticari broiler ve yumurtacı tavuklarda ELISA ile Mycoplasma synoviae seropozitifliği
Yıl: 2022 Cilt: 79 Sayı: 4 Sayfa Aralığı: 656 - 665 Metin Dili: Türkçe DOI: 10.5505/TurkHijyen.2022.20846 İndeks Tarihi: 30-12-2022
Ticari broiler ve yumurtacı tavuklarda ELISA ile Mycoplasma synoviae seropozitifliği
Öz: ÖZET Amaç: Mycoplasma synoviae (M. synoviae), dünya genelinde kanatlı endüstrisinde önemli ekonomik kayıplara neden olan, mikoplazma türleri içerisinde klinik açıdan en önemli olan ikinci türdür. Bu çalışmanın amacı, ticari broiler ve ticari yumurtacı sürülerde M. synoviae’ya karşı oluşan antikorların varlığını indirek ELISA ile araştırmaktır. Yöntem: İç Anadolu Bölgesi’nde aşısız ticari broiler (n=380) ve ticari yumurtacı (n=577) sürülerden tesadüfi örnekleme yoluyla aseptik koşullarda vena subcutanae ulnaristen toplam 957 kan örneği toplandı ve serumları ayrıldı. Serum örneklerinde anti-M. synoviae antikorları ticari indirekt ELISA kiti ile belirlendi. Bulgular: Yirmibir ticari broiler sürüsünden alınan 380 ve 33 ticari yumurtacı sürüsünden alınan 577 kan serumu örneğinde sırasıyla seropozitiflik oranı %40,76 (n=155) ve %54,59 (n=315) olarak saptandı. Sonuç: İç Anadolu Bölgesi’nde kanatlı yetiştiriciliğinde özellikle ticari yumurtacı sürülerde, broilerlere göre M. synoviae, enfeksiyonunun daha yaygın olduğu belirlendi. Her iki yetiştirme tipinde M. synoviae kaynaklı ekonomik kayıp risklerinin oluşmaması için kanatlı damızlık sürülerin biyogüvenlik önemlerinin arttırılması, özellikle damızlık sürülerde izleme çalışmaları ile düzenli takiplerin yapılması ve enfeksiyon varlığı saptanmış sürülerde en kısa sürede tedavi uygulamasının önem arzettiği kanaatindeyiz.
Anahtar Kelime: Seropositivity of Mycoplasma synoviae by ELISA in commercial broiler and layer chickens
Öz: ABSTRACT Objective: Mycoplasma synoviae (M. synoviae)is the second most clinically important mycoplasma species, causing significant economic losses to the poultry industry worldwide. The aim of this study is to investigate the presence of antibodies against M. synoviae by indirect ELISA in commercial broiler and commercial layer flocks. Methods: A total of 957 blood samples were collected from vena subcutanae ulnaris under aseptic conditions by random sampling from unvaccinated commercial broiler (n=380) and commercial layer (n=577) flocks in the Central Anatolia Region and their serums were extracted. Anti-M.synoviae antibodies were detected by using commercial indirect ELISA kit. Results: The seropositivity rates were determined 40.76% (n=155) and 54.59% (n=315) in 380 sera from 21 commercial broiler flocks and 577 sera from 33 commercial layer flocks, respectively. Conclusion: In comparison to broilers, M. synoviae infection was shown to be more widespread in poultry breeding, particularly in commercial layer flocks, in the Central Anatolia Region. To avoid economic loss due to M. synoviae in both breeding types, we conclude that it is critical to improve the biosecurity of poultry breeder flocks, conduct regular follow-ups with monitoring studies, especially in breeder flocks, and treat infected herds as soon as possible.
Anahtar Kelime: Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
- 1. Feberwee A, Morrow C J, Ghorashi S A, Noormohammadi A H, Landman W J. Effect of a live Mycoplasma synoviae vaccine on the production of eggshell apex abnormalities induced by a M. synoviae infection preceded by an infection with infectious bronchitis virus D1466. Avian Pathol, 2009; 38:333–40.
- 2. Lierz M, Hagen N, Harcourt-Brown N, Hernandez-Divers SJ, Lüschow D, Hafez HM. Prevalence of mycoplasmas in eggs from birds of prey using culture and a genus-specific mycoplasma polymerase chain reaction. Avian Pathol, 2007;36(2):145-50.
- 3. Catania S, Bilato D, Gobbo F, Granato A, Terregino C, Iob L, et al. Treatment of eggshell abnormalities and reduced egg production caused by Mycoplasma synoviae infection. Avian Dis, 2010;54(2):961-4.
- 4. Landman WJ. Is Mycoplasma synoviae outrunning Mycoplasma gallisepticum? A viewpoint from the Netherlands. Avian Pathol, 2014; 43(1):2-8.
- 5. Feberwee A, De Wit JJ, Landman WJ. Induction of eggshell apex abnormalities by Mycoplasma synoviae: field and experimental studies. Avian Pathol, 2009; 38(1):77-85.
- 6. Dufour-Gesbert F, Dheilly A, Marois C, Kempf I. Epidemiological study on Mycoplasma synoviae infection in layers. Vet Microbiol, 2006;114(1-2):148-54.
- 7. Moreira FA, Cardoso L, Coelho AC. Decreased production in broiler breeders due to tendon rupture by Mycoplasma synoviae. J Hell Vet Med, 2014;65(2):109-14.
- 8. Xue J, Xu MY, Ma ZJ, Zhao J, Jin N, Zhang GZ. Serological investigation of Mycoplasma synoviae infection in China from 2010 to 2015. Poult Sci, 2017; 96(9):3109-12.
- 9. Ferguson Noel N, Armour NK, Noormohammadi AH, El Gazzar M, Bradbury JM. Mycoplasmosis. Dis Poult, 2020;13:907-65.
- 10. Marois C, Oufour-Gesbert F, Kempf I. Detection of Mycoplasma synoviae in poultry environment samples by culture and polymerase chain reaction. Vet Microbiol, 2000; 11;73(4):311-8.
- 11. Ewing ML, Cookson KC, Phillips RA, Turner KR, Kleven SH. Experimental infection and transmissibility of Mycoplasma synoviae with delayed serologic response in chickens. Avian Dis, 1998;1:230-8.
- 12. Kleven SH (2003) Mycoplasma synoviae. In: Saif YM (ed) Diseases of poultry, 11th edn. Iowa State University Press, 2003, pp 756–65.
- 13. Nascimento ER, Pereira VL, Nascimento MG, Barreto ML. Avian mycoplasmosis update. Braz J Poult Sci, 2005 ;7(1):1-9.
- 14. Sun S, Lin X, Liu J, Tian Z, Chen F, Cao Y, et al. Phylogenetic and pathogenic analysis of Mycoplasma synoviae isolated from native chicken breeds in China. BMC Genom. 2017 ;96(7):2057-63.
- 15. Alam J, Muhammad F, Siddiqui MU, Khan SA, Rehmani S, Ahmad A. Dot-ELISA for Newcastle disease, infectious Bursal disease and mycoplasmosis. Pak J Zool, 2012; 44(5): 1301-5.
- 16. Ghadimipour R, Gharibi D, Mayahi M. Detection of Mycoplasma gallisepticum and Mycoplasma synoviae among commercial poultry in Khouzestan province, Iran. Arch Razi Inst, 2018; 73:139–46.
- 17. Ewing ML, Lauerman LH, Kleven SH, Brown MB. Evaluation of diagnostic procedures to detect Mycoplasma synoviae in commercial multiplier-breeder farms and commercial hatcheries in Florida. Avian Dis, 1996;1:798-806.
- 18. Gole VC, Chousalkar KK, Roberts JR. Prevalence of antibodies to Mycoplasma synoviae in laying hens and possible effects on egg shell quality. Prev Vet Med, 2012;106:75–8.
- 19. Luciano RL, Cardoso AL, Stoppa GF, Kanashiro AM, De Castro AG, Tessari EN. Comparative study of serological tests for Mycoplasma synoviae diagnosis in commercial poultry breeders. Vet Med Int, 2011;3;2011.
- 20. Ferguson-Noel SHKaN. Mycoplasma synoviae infection. In: Disease of Poultry (Chinese Version). Edited by Saif.Y.M, 12th ed. Beijing: Blackwell Publishing 2008; 999–1014.
- 21. Hagan JC, Ashton N J, Bradbury JM, Morgan K L. Evaluation of an egg yolk enzyme-linked immunosorbent assay antibody test and its use to assess the prevalence of Mycoplasma synoviae in UK laying hens. Avian Pathol, 2004;33:93–7.
- 22. Catania S, Gobbo F, Bilato D, Gagliazzo L, Moronato ML, Terregino C, et al. Two strains of Mycoplasma synoviae from chicken flocks on the same layer farm differ in their ability to produce eggshell apexabnormality. Vet Microbiol, 2016;193:60–6.
- 23. Cortés V, Sevilla-Navarro S, García C, Tudón A, Marín C, Catalá-Gregori P. Seroprevalence and prevalence of Mycoplasma synoviae in laying hens and broiler breeders in Spain. Poult Sci, 2021;100(3):100911.
- 24. Fiorentin L, Soncini RA, da Costa JL, Mores MA, Trevisol IM, Toda M, et al. Apparent eradication of Mycoplasma synoviae in broiler breeders subjected to intensive antibiotic treatment directed to control Escherichia coli. Avian Pathol, 2003;32(2):213-6.
- 25. Cobb SP. The spread of pathogens through trade in poultry hatching eggs: overview and recent developments. Rev Sci Tech Off Int Epiz, 2011;30(1): 165-75.
- 26. OIE, Animal Health World Organization,”Avian mycoplasmosis,”in Manuel of Standards for Diagnostic Tests and Vaccines, chapter 2.3.5,pp 482-496,2008.
- 27. Rasool A, Anjum AA, Rabbani M, Lateef M, Nawaz M, Akhtar F, et al. Preparation of Mycoplasma synoviae antigens and evaluation by rapid slide agglutination and enzyme linked immunosorbent assay. J Anim Plant Sci, 2017; 1;27(3).
- 28. Haghighi-Khoshkhoo P, Akbariazad G, Roohi M, Inanlo J, Masoumi M, Sami-Yousefi P. Seroprevalence of Mycoplasma gallisepticum and Mycoplasma synoviae infection in the commercial layer flocks of the centernorth of Iran. Afr J Microbiol Res, 2011; 5(18): 2834-7.
- 29. Nassik S, Aboukhalid R, Azzam F, Rahmatallah N, Lahlou-Amine I, Fassi-Fihri O, et al. Detection of Mycoplasma synoviae infection in broiler breeder farms of morocco using serological assays and real time PCR. J Life Sci, 2014;8: 815-21.
- 30. Akan M. Tavuklarda mikoplazma infeksiyonları: koruma ve kontrol. Veteriner Tavukçuluk Derneği Mektup Ankara, 2008; 6: 21-4.
- 31. Dakman A, Günaydın E, Türkyılmaz MA, Güleç M, Coşar M, Özdemir Ü. Damızlık tavuk işletmelerinde tespit edilen mikoplazma infeksiyonları. J Etlik Vet Microbiol, 2009;20:27-34.
- 32. Özgün MR, Türkyılmaz S. The Determination of Mycoplasma gallisepticum and Mycoplasma synoviae antibodies in poultry blood sera with ELISA. Kocatepe Vet J, 2016;9(1):19-23.
- 33. Mohammed HO, Carpenter TE, Yamamoto R, McMartin DA. Prevalence of Mycoplasma gallisepticum and M. synoviae in commercial layers in southern and central California. Avian Dis, 1986; 30: 519-26.
- 34. Feberwee A, de Vries TS, Landman WJ. Seroprevalence of Mycoplasma synoviae in Dutch commercial poultry farms. Avian Pathol, 2008;37(6):629-33.
- 35. Atique MA, Abbas F, Awan MA, Tariq MM, Ahmed Z, Ali I, et al. Identification of avian Mycoplasma species in commercial broilers and layers with respiratory symptoms in Balochistan. Afr J Biotechnol, 2012;11(100):16557-9.
- 36. Kapetanov M, Orlic D, Potkonjak DU, Velhner MA, Stojanov I, Milanov DU, et al. Mycoplasma in poultry flocks in the year 2009 compared to the year 2000 and significance of the control measures. Lucrari Sci Med Vet, 2010; 43:249–53.
- 37. El-Ashram S, Hashad ME, Abdel-Alim GA, Abdelhamid T, N. Deif H. Seroprevalence of mycoplasmosis in broiler, layer, and native chickens in Giza, Egypt. PLoS One, 2021; 16(7):e0254220.
- 38. Buim MR, Mettifogo E, Timenetsky J, Kleven S, Ferreira AJ. Epidemiological survey on Mycoplasma gallisepticum and M. synoviae by multiplex PCR in commercial poultry. Pesq Vet Bras, 2009;29:552–6.
- 39. Suzuki, K., J. Origlia, F. Alvaez, M. Faccioli, M. Silva, J. Caballero, et al. Relative risk estimation for Mycoplasma synoviae in backyard chickens in Paraguay. Int J Poult Sci, 2009; 8:842–7.
- 40. Stipkovits L, Kempf I. Mycoplasmoses in poultry. Revue scientifique et technique (International Office of Epizootics), 1996;15(4):1495-525.
- 41. Eissa S. Preparation and evaluation of Mycoplasma gallisepticum and Mycoplasma synoviae recombinant vaccine. Zagazig Vet J, 2019;47(1):1-10.
| APA | GONCAGÜL G, Gunaydin E, kardoğan ö, ÇOKAL Y (2022). Ticari broiler ve yumurtacı tavuklarda ELISA ile Mycoplasma synoviae seropozitifliği. , 656 - 665. 10.5505/TurkHijyen.2022.20846 |
| Chicago | GONCAGÜL GÜLSEN,Gunaydin Elcin,kardoğan özlem,ÇOKAL YAVUZ Ticari broiler ve yumurtacı tavuklarda ELISA ile Mycoplasma synoviae seropozitifliği. (2022): 656 - 665. 10.5505/TurkHijyen.2022.20846 |
| MLA | GONCAGÜL GÜLSEN,Gunaydin Elcin,kardoğan özlem,ÇOKAL YAVUZ Ticari broiler ve yumurtacı tavuklarda ELISA ile Mycoplasma synoviae seropozitifliği. , 2022, ss.656 - 665. 10.5505/TurkHijyen.2022.20846 |
| AMA | GONCAGÜL G,Gunaydin E,kardoğan ö,ÇOKAL Y Ticari broiler ve yumurtacı tavuklarda ELISA ile Mycoplasma synoviae seropozitifliği. . 2022; 656 - 665. 10.5505/TurkHijyen.2022.20846 |
| Vancouver | GONCAGÜL G,Gunaydin E,kardoğan ö,ÇOKAL Y Ticari broiler ve yumurtacı tavuklarda ELISA ile Mycoplasma synoviae seropozitifliği. . 2022; 656 - 665. 10.5505/TurkHijyen.2022.20846 |
| IEEE | GONCAGÜL G,Gunaydin E,kardoğan ö,ÇOKAL Y "Ticari broiler ve yumurtacı tavuklarda ELISA ile Mycoplasma synoviae seropozitifliği." , ss.656 - 665, 2022. 10.5505/TurkHijyen.2022.20846 |
| ISNAD | GONCAGÜL, GÜLSEN vd. "Ticari broiler ve yumurtacı tavuklarda ELISA ile Mycoplasma synoviae seropozitifliği". (2022), 656-665. https://doi.org/10.5505/TurkHijyen.2022.20846 |
| APA | GONCAGÜL G, Gunaydin E, kardoğan ö, ÇOKAL Y (2022). Ticari broiler ve yumurtacı tavuklarda ELISA ile Mycoplasma synoviae seropozitifliği. Türk Hijyen ve Deneysel Biyoloji Dergisi, 79(4), 656 - 665. 10.5505/TurkHijyen.2022.20846 |
| Chicago | GONCAGÜL GÜLSEN,Gunaydin Elcin,kardoğan özlem,ÇOKAL YAVUZ Ticari broiler ve yumurtacı tavuklarda ELISA ile Mycoplasma synoviae seropozitifliği. Türk Hijyen ve Deneysel Biyoloji Dergisi 79, no.4 (2022): 656 - 665. 10.5505/TurkHijyen.2022.20846 |
| MLA | GONCAGÜL GÜLSEN,Gunaydin Elcin,kardoğan özlem,ÇOKAL YAVUZ Ticari broiler ve yumurtacı tavuklarda ELISA ile Mycoplasma synoviae seropozitifliği. Türk Hijyen ve Deneysel Biyoloji Dergisi, vol.79, no.4, 2022, ss.656 - 665. 10.5505/TurkHijyen.2022.20846 |
| AMA | GONCAGÜL G,Gunaydin E,kardoğan ö,ÇOKAL Y Ticari broiler ve yumurtacı tavuklarda ELISA ile Mycoplasma synoviae seropozitifliği. Türk Hijyen ve Deneysel Biyoloji Dergisi. 2022; 79(4): 656 - 665. 10.5505/TurkHijyen.2022.20846 |
| Vancouver | GONCAGÜL G,Gunaydin E,kardoğan ö,ÇOKAL Y Ticari broiler ve yumurtacı tavuklarda ELISA ile Mycoplasma synoviae seropozitifliği. Türk Hijyen ve Deneysel Biyoloji Dergisi. 2022; 79(4): 656 - 665. 10.5505/TurkHijyen.2022.20846 |
| IEEE | GONCAGÜL G,Gunaydin E,kardoğan ö,ÇOKAL Y "Ticari broiler ve yumurtacı tavuklarda ELISA ile Mycoplasma synoviae seropozitifliği." Türk Hijyen ve Deneysel Biyoloji Dergisi, 79, ss.656 - 665, 2022. 10.5505/TurkHijyen.2022.20846 |
| ISNAD | GONCAGÜL, GÜLSEN vd. "Ticari broiler ve yumurtacı tavuklarda ELISA ile Mycoplasma synoviae seropozitifliği". Türk Hijyen ve Deneysel Biyoloji Dergisi 79/4 (2022), 656-665. https://doi.org/10.5505/TurkHijyen.2022.20846 |
