Ratlarda Siklofosfamid ile Oluşturulan Anemi Modelinde Zeytin Yaprağı Ekstraktının Etkilerinin Araştırılması

GÜVENÇ, MEHMET; Etyemez, Muhammed

Ratlarda Siklofosfamid ile Oluşturulan Anemi Modelinde Zeytin Yaprağı Ekstraktının Etkilerinin Araştırılması

Mehmet GÜVENÇ, (Hatay Mustafa Kemal Üniversitesi, Veteriner Fakültesi, Fizyoloji Ana Bilim Dalı, Hatay, Türkiye)

Muhammed ETYEMEZ (Kastamonu Üniversitesi, Veteriner Fakültesi, Fizyoloji Ana Bilim Dalı, Kastamonu, Türkiye)

Fırat Üniversitesi Sağlık Bilimleri Veteriner Dergisi

20 3

Yıl: 2023 Cilt: 37 Sayı: 2 Sayfa Aralığı: 87 - 92 Metin Dili: Türkçe İndeks Tarihi: 19-07-2023

Ratlarda Siklofosfamid ile Oluşturulan Anemi Modelinde Zeytin Yaprağı Ekstraktının Etkilerinin Araştırılması

Öz:

Siklofosfamid (CP), kanser, multipl skleroz ve romatoid artrit gibi hastalıkların tedavisinde kullanılan ve çeşitli organ hasarları, kemik iliği toksisitesi ve hematopoietik disfonksiyon gibi yan etkilere neden olduğu için kullanımı sınırlı olan bir ilaçtır. Bu çalışmada ratlarda siklofosfamid uygulamasının neden olduğu anemiye karşı zeytin yaprağı ekstraktının (ZYE) koruyucu etkilerinin araştırılması amaçlandı. Çalışma toplam 28 gün sürdü. Bu amaçla anemi modeli oluşturulan grupta 50 mg/kg dozda, haftada bir kez ve intramusküler olarak CP uygulaması yapıldı. Tedavi grubunda ise oral gavaj yöntemi ile 400 mg/kg dozda ZYE uygulaması yapıldı. Çalışma sonunda hematolojik analizlerde alyuvar (RBC), akyuvar (WBC), hemoglobin konsantrasyonu (HGB), hematokrit değer (HTC), ortalama alyuvar hemoglobini (MCH), ortalama alyuvar hacmi (MCV), eritrosit dağılım genişliği (RDW) gibi parametreler incelendi. Serum örneklerinde ise malondialdehid (MDA) ve redükte glutatyon (GSH) düzeyleri ile katalaz (CAT) ve glutatyon peroksidaz (GSH-Px) enzim aktiviteleri spektrofotometrik yöntemle analiz edildi. CP uygulamasının kan RBC (P<0.01), HGB (P<0.001) ve HTC (P<0.001) parametrelerinde istatistiki açıdan anlamlı azalmalara neden olduğu belirlendi. Ayrıca bu uygulamanın serum MDA (P<0.05) düzeyinde artışa, GSH (P<0.05) düzeyi ve CAT (P<0.05) ve GSH-Px (P<0.01) enzim aktivitelerinde azalmaya sebep olarak oksidatif stres oluşturduğu tespit edildi. Zeytin yaprağı ekstrakt tedavisinin ise CP’nin neden olduğu oksidatif hasarı önlediği, ayrıca RBC, HGB ve HTC değerlerindeki azalmanın önüne geçtiği ve anemiye karşı koruyucu etkiler gösterdiği belirlendi.

Anahtar Kelime: Siklofosfamid anemi zeytin yaprağı

Investigation of the Effects of Olive Leaf Extract in a Model of Cyclophosphamide- Induced Anemia in Rats

Öz:

Cyclophosphamide (CP) is a drug used in the treatment of diseases such as cancer and rheumatoid arthritis, and its use is limited because it causes side effects such as various organ damage, bone marrow toxicity, and hematopoietic dysfunction. In this study, it was aimed to investigate the protective effects of olive leaf extract (ZYE) against anemia in rats. The study period was 28 days. For this purpose, CP was administered at a dose of 50 mg/kg once a week intramuscularly in the group in which anemia model was created. In the treatment group, 400 mg/kg ZYE was administered by oral gavage method. At the end of the study, red blood cells (RBC), white blood cells (WBC), hemoglobin concentration (HGB), hematocrit value (HTC), mean red blood cell hemoglobin (MCH), mean red blood cell volume (MCHC) and red blood cell distribution (RDW) were determined. In serum samples, malonyldialdehyde (MDA) and reduced glutathione (GSH) levels, catalase (CAT) and glutathione peroxidase (GSH-Px) enzyme activities were analyzed by spectrophotometric method. It was determined that CP administration caused statistically significant decreases in blood RBC (P<0.01), HGB (P<0.001) and HTC (P<0.001) parameters. In addition, it was determined that this application caused an increase in serum MDA (P<0.05) level, a decrease in GSH (P<0.05) level and CAT (P<0.05) and GSH-Px (P<0.01) enzyme activities, resulting in oxidative stress. It was determined that olive leaf extract treatment prevented the oxidative damage caused by CP, also prevented the decrease in RBC, WBC, HGB and HTC values and showed protective effects against anemia.

Anahtar Kelime: Cyclophosphamide anemia olive leaf

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	GÜVENÇ M, Etyemez M (2023). Ratlarda Siklofosfamid ile Oluşturulan Anemi Modelinde Zeytin Yaprağı Ekstraktının Etkilerinin Araştırılması. , 87 - 92.
Chicago	GÜVENÇ MEHMET,Etyemez Muhammed Ratlarda Siklofosfamid ile Oluşturulan Anemi Modelinde Zeytin Yaprağı Ekstraktının Etkilerinin Araştırılması. (2023): 87 - 92.
MLA	GÜVENÇ MEHMET,Etyemez Muhammed Ratlarda Siklofosfamid ile Oluşturulan Anemi Modelinde Zeytin Yaprağı Ekstraktının Etkilerinin Araştırılması. , 2023, ss.87 - 92.
AMA	GÜVENÇ M,Etyemez M Ratlarda Siklofosfamid ile Oluşturulan Anemi Modelinde Zeytin Yaprağı Ekstraktının Etkilerinin Araştırılması. . 2023; 87 - 92.
Vancouver	GÜVENÇ M,Etyemez M Ratlarda Siklofosfamid ile Oluşturulan Anemi Modelinde Zeytin Yaprağı Ekstraktının Etkilerinin Araştırılması. . 2023; 87 - 92.
IEEE	GÜVENÇ M,Etyemez M "Ratlarda Siklofosfamid ile Oluşturulan Anemi Modelinde Zeytin Yaprağı Ekstraktının Etkilerinin Araştırılması." , ss.87 - 92, 2023.
ISNAD	GÜVENÇ, MEHMET - Etyemez, Muhammed. "Ratlarda Siklofosfamid ile Oluşturulan Anemi Modelinde Zeytin Yaprağı Ekstraktının Etkilerinin Araştırılması". (2023), 87-92.

APA	GÜVENÇ M, Etyemez M (2023). Ratlarda Siklofosfamid ile Oluşturulan Anemi Modelinde Zeytin Yaprağı Ekstraktının Etkilerinin Araştırılması. Fırat Üniversitesi Sağlık Bilimleri Veteriner Dergisi, 37(2), 87 - 92.
Chicago	GÜVENÇ MEHMET,Etyemez Muhammed Ratlarda Siklofosfamid ile Oluşturulan Anemi Modelinde Zeytin Yaprağı Ekstraktının Etkilerinin Araştırılması. Fırat Üniversitesi Sağlık Bilimleri Veteriner Dergisi 37, no.2 (2023): 87 - 92.
MLA	GÜVENÇ MEHMET,Etyemez Muhammed Ratlarda Siklofosfamid ile Oluşturulan Anemi Modelinde Zeytin Yaprağı Ekstraktının Etkilerinin Araştırılması. Fırat Üniversitesi Sağlık Bilimleri Veteriner Dergisi, vol.37, no.2, 2023, ss.87 - 92.
AMA	GÜVENÇ M,Etyemez M Ratlarda Siklofosfamid ile Oluşturulan Anemi Modelinde Zeytin Yaprağı Ekstraktının Etkilerinin Araştırılması. Fırat Üniversitesi Sağlık Bilimleri Veteriner Dergisi. 2023; 37(2): 87 - 92.
Vancouver	GÜVENÇ M,Etyemez M Ratlarda Siklofosfamid ile Oluşturulan Anemi Modelinde Zeytin Yaprağı Ekstraktının Etkilerinin Araştırılması. Fırat Üniversitesi Sağlık Bilimleri Veteriner Dergisi. 2023; 37(2): 87 - 92.
IEEE	GÜVENÇ M,Etyemez M "Ratlarda Siklofosfamid ile Oluşturulan Anemi Modelinde Zeytin Yaprağı Ekstraktının Etkilerinin Araştırılması." Fırat Üniversitesi Sağlık Bilimleri Veteriner Dergisi, 37, ss.87 - 92, 2023.
ISNAD	GÜVENÇ, MEHMET - Etyemez, Muhammed. "Ratlarda Siklofosfamid ile Oluşturulan Anemi Modelinde Zeytin Yaprağı Ekstraktının Etkilerinin Araştırılması". Fırat Üniversitesi Sağlık Bilimleri Veteriner Dergisi 37/2 (2023), 87-92.