Jaceosidin protects L929 fibroblast cells by down-regulation of proinflammatory cytokines and attenuation of oxidative stress-induced impairment of cell proliferation and migration

Büşra DİNÇER, (Ondokuz Mayıs Üniversitesi, Eczacılık Fakültesi, Eczacılık Anabilim Dalı, Samsun, Türkiye)
Azat ÇEKDAR GÜNDOĞMUŞ, (Erzincan Binali Yıldırım Üniversitesi, Eczacılık Fakültesi, Eczacılık Anabilim Dalı, Erzincan, Türkiye)
İrfan ÇINAR (Kastamonu Üniversitesi, Tıp Fakültesi, Eczacılık Anabilim Dalı, Kastamonu, Türkiye)
Experimental Biomedical Research
18 0
Yıl: 2023 Cilt: 6 Sayı: 3 Sayfa Aralığı: 238 - 248 Metin Dili: İngilizce DOI: 10.30714/j-ebr.2023.188 İndeks Tarihi: 21-07-2023

Jaceosidin protects L929 fibroblast cells by down-regulation of proinflammatory cytokines and attenuation of oxidative stress-induced impairment of cell proliferation and migration

Öz:
Aim: Oxidative stress has been a significant factor in wound-healing pathophysiology for a long time. Antioxidants, especially natural compounds, have recently been emphasized in instructions for wound healing treatments. Jaceosidin (JACE), a flavone derived from Artemisia princeps, is a potent antioxidant. This study aims to investigate JACE’s anti-inflammatory and antioxidant properties and its capacity to improve the effects of in vitro wound healing. Methods: Wound healing activities have been tested using cell proliferation and migration in vitro assays in the mouse fibroblast cell line L929. The concentration of hydrogen peroxide (H2O2-0.5 mM) has been used to induce the oxidative stress model. Tumor necrosis factor-alpha (TNF-α) and nuclear factor (NF-) have been investigated as inflammatory indicators. Antioxidant activity has been checked using total antioxidant status (TAS) and total oxidant status (TOS) tests. Results: JACE has significantly increased the proliferation of fibroblasts dose-dependent manner. It has enhanced the cell migration rate of fibroblasts compared with the H2O2 group. JACE at a concentration of 50 and 100 μM has significantly decreased TOS and oxidative stress index (OSI) levels and increased TAS levels. The anti-inflammatory mechanism of JACE has involved down-regulation of the mRNA expressions of the NF- and TNF-α in a dose-dependent manner. Conclusions: JACE has beneficial impacts on fibroblast viability and migration qualities through antioxidative actions and down-regulating proinflammatory cytokines through anti-inflammatory effects to promote wound healing. The present study shows that JACE may help to increase the range of available treatments for woundhealing by reducing inflammation and oxidative stress.
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APA Dincer B, GÜNDOĞMUŞ A, ÇINAR İ (2023). Jaceosidin protects L929 fibroblast cells by down-regulation of proinflammatory cytokines and attenuation of oxidative stress-induced impairment of cell proliferation and migration. , 238 - 248. 10.30714/j-ebr.2023.188
Chicago Dincer Busra,GÜNDOĞMUŞ Azat Çekdar,ÇINAR İRFAN Jaceosidin protects L929 fibroblast cells by down-regulation of proinflammatory cytokines and attenuation of oxidative stress-induced impairment of cell proliferation and migration. (2023): 238 - 248. 10.30714/j-ebr.2023.188
MLA Dincer Busra,GÜNDOĞMUŞ Azat Çekdar,ÇINAR İRFAN Jaceosidin protects L929 fibroblast cells by down-regulation of proinflammatory cytokines and attenuation of oxidative stress-induced impairment of cell proliferation and migration. , 2023, ss.238 - 248. 10.30714/j-ebr.2023.188
AMA Dincer B,GÜNDOĞMUŞ A,ÇINAR İ Jaceosidin protects L929 fibroblast cells by down-regulation of proinflammatory cytokines and attenuation of oxidative stress-induced impairment of cell proliferation and migration. . 2023; 238 - 248. 10.30714/j-ebr.2023.188
Vancouver Dincer B,GÜNDOĞMUŞ A,ÇINAR İ Jaceosidin protects L929 fibroblast cells by down-regulation of proinflammatory cytokines and attenuation of oxidative stress-induced impairment of cell proliferation and migration. . 2023; 238 - 248. 10.30714/j-ebr.2023.188
IEEE Dincer B,GÜNDOĞMUŞ A,ÇINAR İ "Jaceosidin protects L929 fibroblast cells by down-regulation of proinflammatory cytokines and attenuation of oxidative stress-induced impairment of cell proliferation and migration." , ss.238 - 248, 2023. 10.30714/j-ebr.2023.188
ISNAD Dincer, Busra vd. "Jaceosidin protects L929 fibroblast cells by down-regulation of proinflammatory cytokines and attenuation of oxidative stress-induced impairment of cell proliferation and migration". (2023), 238-248. https://doi.org/10.30714/j-ebr.2023.188
APA Dincer B, GÜNDOĞMUŞ A, ÇINAR İ (2023). Jaceosidin protects L929 fibroblast cells by down-regulation of proinflammatory cytokines and attenuation of oxidative stress-induced impairment of cell proliferation and migration. Experimental Biomedical Research, 6(3), 238 - 248. 10.30714/j-ebr.2023.188
Chicago Dincer Busra,GÜNDOĞMUŞ Azat Çekdar,ÇINAR İRFAN Jaceosidin protects L929 fibroblast cells by down-regulation of proinflammatory cytokines and attenuation of oxidative stress-induced impairment of cell proliferation and migration. Experimental Biomedical Research 6, no.3 (2023): 238 - 248. 10.30714/j-ebr.2023.188
MLA Dincer Busra,GÜNDOĞMUŞ Azat Çekdar,ÇINAR İRFAN Jaceosidin protects L929 fibroblast cells by down-regulation of proinflammatory cytokines and attenuation of oxidative stress-induced impairment of cell proliferation and migration. Experimental Biomedical Research, vol.6, no.3, 2023, ss.238 - 248. 10.30714/j-ebr.2023.188
AMA Dincer B,GÜNDOĞMUŞ A,ÇINAR İ Jaceosidin protects L929 fibroblast cells by down-regulation of proinflammatory cytokines and attenuation of oxidative stress-induced impairment of cell proliferation and migration. Experimental Biomedical Research. 2023; 6(3): 238 - 248. 10.30714/j-ebr.2023.188
Vancouver Dincer B,GÜNDOĞMUŞ A,ÇINAR İ Jaceosidin protects L929 fibroblast cells by down-regulation of proinflammatory cytokines and attenuation of oxidative stress-induced impairment of cell proliferation and migration. Experimental Biomedical Research. 2023; 6(3): 238 - 248. 10.30714/j-ebr.2023.188
IEEE Dincer B,GÜNDOĞMUŞ A,ÇINAR İ "Jaceosidin protects L929 fibroblast cells by down-regulation of proinflammatory cytokines and attenuation of oxidative stress-induced impairment of cell proliferation and migration." Experimental Biomedical Research, 6, ss.238 - 248, 2023. 10.30714/j-ebr.2023.188
ISNAD Dincer, Busra vd. "Jaceosidin protects L929 fibroblast cells by down-regulation of proinflammatory cytokines and attenuation of oxidative stress-induced impairment of cell proliferation and migration". Experimental Biomedical Research 6/3 (2023), 238-248. https://doi.org/10.30714/j-ebr.2023.188
Sayfa Oluşturulma Tarihi
24-04-2024 8:29

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