Investigation of Q Fever in Kilis and Shamil goatsby ELISA and Touchdown-PCR

Elçin GÜNAYDIN, (Etlik Merkez Veteriner Kontrol ve Araştırma Enstitüsü, Ankara, Türkiye)
Selçuk PEKKAYA, (Etlik Merkez Veteriner Kontrol ve Araştırma Enstitüsü, Ankara, Türkiye)
H. Kaan MÜŞTAK, (Ankara Üniversitesi, Veteriner Fakültesi, Mikrobiyoloji Anabilim Dalı, Ankara, Türkiye)
Bestami DALKILIÇ (Gaziantep Üniversitesi, Gaziantep Meslek Yüksek Okulu, Gaziantep, Türkiye)
Ankara Üniversitesi Veteriner Fakültesi Dergisi
2 0
Yıl: 2014 Cilt: 61 Sayı: 3 Sayfa Aralığı: 161 - 165 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022

Investigation of Q Fever in Kilis and Shamil goatsby ELISA and Touchdown-PCR

Öz:
Bu çalışma, Kilis ve Halep keçilerinde, Q Hummasının farklı dönemlerini -kronik ve akut- sırasıyla; serum örneklerinde indirek ELISA, kan örneklerinde touchdown-PCR ile ortaya koymak için planlanmıştır. Kırkaltı Kilis ve 46 Halep keçisinden oluşan toplam 92 serum örneği incelendi ve toplam serum örnekleri, Kilis ve Halep keçi serumlarında seropoziflik sırasıyla, 7.60%, 4.34% ve 10.8% bulundu. Kırkaltı Kilis ve 46 Halep keçisine ait toplam 92 kan örneğinin, hiçbirinde touchdown-PCR ile 687 bplık PCR ürünü tespit edilmedi. Pozitif ELISA titrelerinin daha once geçirilen infeksiyona kanıt olduğu düşünüldü. Türkiyede Güneydoğu Anadolu Bölgesinde lokalize olan Kilis ilinin Q fever yönünden riskli bir bölge olduğuna karar verildi.
Anahtar Kelime:

Konular: Veterinerlik

Kilis ve Halep keçilerinde ELISA ve Touchdown-PCR ile Q Humması nın araştırılması

Öz:
This study was carried out on Kilis and Shamil goats to determine the different stages of Q fever-chronic and acute- in serum samples by indirect ELISA and in blood samples by touchdown-PCR, respectively. A total of 92 serum samples comprising 46 Kilis and 46 Shamil goats was examined and 7.60%, 4.34% and 10.8% seropositivity were determined for total serum samples, Kilis and Shamil goat serum samples, respectively. Of the examined 92 blood samples belonging to 46 Kilis and 46 Shamil goats, none of them yielded 687 bp PCR products by touchdown-PCR. The positive ELISA titers were thought to be the evidence of previous infection. The province of Kilis localized in South-East Anatolian Region of Turkey was decided to be a risky region for Q fever.
Anahtar Kelime:

Konular: Veterinerlik
Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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APA Gunaydin E, PEKKAYA S, MÜŞTAK H, DALKILIÇ B (2014). Investigation of Q Fever in Kilis and Shamil goatsby ELISA and Touchdown-PCR. , 161 - 165.
Chicago Gunaydin Elcin,PEKKAYA Selçuk,MÜŞTAK H. Kaan,DALKILIÇ Bestami Investigation of Q Fever in Kilis and Shamil goatsby ELISA and Touchdown-PCR. (2014): 161 - 165.
MLA Gunaydin Elcin,PEKKAYA Selçuk,MÜŞTAK H. Kaan,DALKILIÇ Bestami Investigation of Q Fever in Kilis and Shamil goatsby ELISA and Touchdown-PCR. , 2014, ss.161 - 165.
AMA Gunaydin E,PEKKAYA S,MÜŞTAK H,DALKILIÇ B Investigation of Q Fever in Kilis and Shamil goatsby ELISA and Touchdown-PCR. . 2014; 161 - 165.
Vancouver Gunaydin E,PEKKAYA S,MÜŞTAK H,DALKILIÇ B Investigation of Q Fever in Kilis and Shamil goatsby ELISA and Touchdown-PCR. . 2014; 161 - 165.
IEEE Gunaydin E,PEKKAYA S,MÜŞTAK H,DALKILIÇ B "Investigation of Q Fever in Kilis and Shamil goatsby ELISA and Touchdown-PCR." , ss.161 - 165, 2014.
ISNAD Gunaydin, Elcin vd. "Investigation of Q Fever in Kilis and Shamil goatsby ELISA and Touchdown-PCR". (2014), 161-165.
APA Gunaydin E, PEKKAYA S, MÜŞTAK H, DALKILIÇ B (2014). Investigation of Q Fever in Kilis and Shamil goatsby ELISA and Touchdown-PCR. Ankara Üniversitesi Veteriner Fakültesi Dergisi, 61(3), 161 - 165.
Chicago Gunaydin Elcin,PEKKAYA Selçuk,MÜŞTAK H. Kaan,DALKILIÇ Bestami Investigation of Q Fever in Kilis and Shamil goatsby ELISA and Touchdown-PCR. Ankara Üniversitesi Veteriner Fakültesi Dergisi 61, no.3 (2014): 161 - 165.
MLA Gunaydin Elcin,PEKKAYA Selçuk,MÜŞTAK H. Kaan,DALKILIÇ Bestami Investigation of Q Fever in Kilis and Shamil goatsby ELISA and Touchdown-PCR. Ankara Üniversitesi Veteriner Fakültesi Dergisi, vol.61, no.3, 2014, ss.161 - 165.
AMA Gunaydin E,PEKKAYA S,MÜŞTAK H,DALKILIÇ B Investigation of Q Fever in Kilis and Shamil goatsby ELISA and Touchdown-PCR. Ankara Üniversitesi Veteriner Fakültesi Dergisi. 2014; 61(3): 161 - 165.
Vancouver Gunaydin E,PEKKAYA S,MÜŞTAK H,DALKILIÇ B Investigation of Q Fever in Kilis and Shamil goatsby ELISA and Touchdown-PCR. Ankara Üniversitesi Veteriner Fakültesi Dergisi. 2014; 61(3): 161 - 165.
IEEE Gunaydin E,PEKKAYA S,MÜŞTAK H,DALKILIÇ B "Investigation of Q Fever in Kilis and Shamil goatsby ELISA and Touchdown-PCR." Ankara Üniversitesi Veteriner Fakültesi Dergisi, 61, ss.161 - 165, 2014.
ISNAD Gunaydin, Elcin vd. "Investigation of Q Fever in Kilis and Shamil goatsby ELISA and Touchdown-PCR". Ankara Üniversitesi Veteriner Fakültesi Dergisi 61/3 (2014), 161-165.
Sayfa Oluşturulma Tarihi
22-04-2024 19:55

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