The Protective Effects of Osajin on Ischemia/Reperfusion Injury to Rat Ovaries: Biochemical and Histopathological Evaluation

ANIL, BARIŞ; keleş, osman nuri; Ozgül  Abuc, Ozlem; ÜNAL, Bünyami; HALICI, Mesut; Can, Dr İsmail; ŞAHİN, Zişan Gönül; ÇAKIR, AHMET; SAĞIR, Dilek; CAN, Serpil; Çığşar, Gülşen; KARACA, MEHMET; Erol, Huseyin Serkan

The Protective Effects of Osajin on Ischemia/Reperfusion Injury to Rat Ovaries: Biochemical and Histopathological Evaluation

Gülşen ÇIĞŞAR, (Kafkas Üniversitesi, Tıp Fakültesi, Acil Bölümü, Kars, Türkiye)

Osman Nuri KELEŞ, (Atatürk Üniversitesi, Tıp Fakültesi, Histoloji ve Embriyoloji Anabilim Dalı, Erzurum, Türkiye)

Serpil CAN, (Kafkas Üniversitesi, Tıp Fakültesi, Fizyoloji Anabilim Dalı, Kars, Türkiye)

Mehmet KARACA, (Medstar Antalya Hastanesi Kadın Hastalıkları Kliniği, Antalya, Türkiye)

İsmail CAN, (Kafkas Üniversitesi, Tıp Fakültesi, Histoloji ve Embriyoloji Bölümü, Kars, Türkiye)

Hüseyin Serkan EROL, (Atatürk Üniversitesi, Veteriner Fakültesi, Biyokimya Ana Bilim Dalı, Erzurum, Türkiye)

Dilek SAĞIR, (Sinop Üniversitesi, Sağlık Bilimleri Fakültesi, Hemşirelik Anabilim Dalı, Sinop, Türkiye)

Özlem Özgül ABUÇ, (Atatürk Üniversitesi, Tıp Fakültesi, Histoloji ve Embriyoloji Anabilim Dalı, Erzurum, Türkiye)

Ahmet ÇAKIR, (Kilis 7 Aralık Üniversitesi, Bilim ve Sanat, Kimya Anabilim Dalı, Kilis,Türkiye)

Zişan Gönül ŞAHİN, (Kafkas Üniversitesi, Tıp Fakültesi, Tıbbi Biyoloji Anabilim Dalı, Kars, Türkiye)

Bünyami ÜNAL, (Atatürk Üniversitesi, Tıp Fakültesi, Histoloji ve Embriyoloji Anabilim Dalı, Erzurum, Türkiye)

Barış ANIL, (Atatürk Üniversitesi, Fen Fakültesi, Kimya Bölümü, Erzurum, Türkiye)

Mesut Bünyami HALICI, (Atatürk Üniversitesi, Veteriner Fakültesi, Biyokimya Ana Bilim Dalı, Erzurum, Türkiye)

Diğer Yazarlar

Kafkas Üniversitesi Veteriner Fakültesi Dergisi

8 2

Yıl: 2015 Cilt: 21 Sayı: 5 Sayfa Aralığı: 753 - 760 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022

The Protective Effects of Osajin on Ischemia/Reperfusion Injury to Rat Ovaries: Biochemical and Histopathological Evaluation

Öz:

İnflamasyon ve Reaktif Oksijen Sınıfları (ROS) iskemi reperfüzyon (I/R) over hasarlarında çok önemli rol oynar. Bu in vivo çalışmanın amacı tek taraflıI/R ile oluşturulan over hasarı ve antiinflamatuvar ve antioksidant özellikli bir flavonoid olan osaj inin oksidatif denge üzerine etkilerini incelemektir.Çalışmada 48 yetişkin dişi Wistar albino rat kullanıldı. Kontrol (CN) grubuna yalnızca laparatomi uygulandı. CNOsaj in grubunda, sadece 200 mg/kgdozda osaj in verildi. IRVEHICLE grubunda, 3 saatlik iskemik periyodu 3 saatlik reperfüzyon takip etti; daha sonra bilateral yumurtalıklar alındı. IROsaj in100 veIROsaj in200 gruplarında 3 saatlik iskemiden sonra sırasıyla 100 ve 200 mg/kg osaj in reperfüzyondan önce oral olarak verildi.; 3 saatlik reperfüzyondansonra yumurtalıklar alındı. Deneylerden sonra, PMNlerin aktiviteleri ve oksidatif durumları için MPO, SOD ve CAT enzim aktiviteleri ve LPO düzeyleribelirlendi. Ek olarak histopatoloj ik değişimler tüm rat ovaryum dokularında incelendi. İstatistiksel analizler one-way ANOVA kullanılarak yapıldı(Duncan ile). Biyokimyasal ve histopatoloj ik sonuçlara göre, yüksek antioksidan SOD ve CAT enzim aktivitesine rağmen, I/R PMNlerin infiltrasyonunu,MPO aktivitelerini ve LPO düzeylerini artırdı. İskemik reperfüzyondan önce, osaj inin her doz seviyesi büyük bir düşüşe sebep olan yüksek dozlabirlikte IRVEHICLE grubunda olanlarla karşılaştırıldığında LPO düzeyi, MPO aktivitesi ve PMN infiltrasyonunu önemli derecede düşürdü. Ek olarak sonuçlardönüşümsüz ovaryum hasarının iyileştirmeden gelişmesine karşın osaj in ile tedavi I/R tarafından uyarılmış olduğunu gösterdi. Bu sonuçlar osaj ininovariyan iskemik reperfüzyon hasarına karşı koruma sağladığını düşündürmektedir. Bunun mekanizmaları oksidatif stresi ve aktivitelerini ve PMNinfiltrasyonunu azaltması ile ilgili olabilir.

Anahtar Kelime:

Konular: Veterinerlik

Sıçan Ovaryumlarında İskemi/Reperfüzyon Hasarı Üzerine Osajin in Koruyucu Etkileri: Biyokimyasal ve Histopatolojik Değerlendirme

Öz:

Reactive oxygen species (ROS) and inflammation play important roles in the pathogenesis of ischemia/reperfusion (I/R) ovarian inj ury. The purposeof this in-vivo study is to evaluate the effect of osaj in, a prenylated flavonoid with antioxidant and anti-inflammatory properties, on oxidativebalance and ovarian damage induced by unilateral I/R. The study used 48 adult, female Wistar albino rats. In the controls (CN), only laparotomy wasperformed. In group CNOsaj in, 200 mg/kg osaj in was administered. In group IRVEHICLE, an ischemic period of 3 h was followed by reperfusion for 3 h;the bilateral ovaries were then removed. In groups IROsaj in100 and IROsaj in200, after 3 h of ischemia, 100 and 200 mg/kg of osaj in was given orally beforereperfusion, respectively; after 3 h of reperfusion, the ovaries were removed. After the experiments, MPO, SOD and CAT enzyme activities and LPOlevels was determined for the oxidative state and activities of PMNs. In addition, histopathological changes were examined in all rat ovarian tissues.Statistical analysis was performed using one-way ANOVA (with Duncan). According to biochemical and histopathological results, I/R increased LPOlevels and MPO activities and infiltration of PMNs despite high-antioxidant SOD and CAT enzyme activity. Both dosage levels of osaj in before I/Rsignificantly decreased LPO level and MPO activity and PMN infiltration compared to those of the IRVEHICLE group, with the higher dosage causinggreater decreases. In addition, results showed that treatment with osaj in against ameliorated development of irreversible ovarian damage inducedby I/R. These results suggest that osaj in provides protections against ovarian I/R inj ury. Its mechanisms could be related to mitigation of oxidativestress and activities and to PMN infiltration.

Anahtar Kelime:

Konular: Veterinerlik

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	Çığşar G, keleş o, CAN S, KARACA M, Can D, Erol H, SAĞIR D, Ozgül Abuc O, ÇAKIR A, ŞAHİN Z, ÜNAL B, ANIL B, HALICI M (2015). The Protective Effects of Osajin on Ischemia/Reperfusion Injury to Rat Ovaries: Biochemical and Histopathological Evaluation. , 753 - 760.
Chicago	Çığşar Gülşen,keleş osman nuri,CAN Serpil,KARACA MEHMET,Can Dr İsmail,Erol Huseyin Serkan,SAĞIR Dilek,Ozgül Abuc Ozlem,ÇAKIR AHMET,ŞAHİN Zişan Gönül,ÜNAL Bünyami,ANIL BARIŞ,HALICI Mesut The Protective Effects of Osajin on Ischemia/Reperfusion Injury to Rat Ovaries: Biochemical and Histopathological Evaluation. (2015): 753 - 760.
MLA	Çığşar Gülşen,keleş osman nuri,CAN Serpil,KARACA MEHMET,Can Dr İsmail,Erol Huseyin Serkan,SAĞIR Dilek,Ozgül Abuc Ozlem,ÇAKIR AHMET,ŞAHİN Zişan Gönül,ÜNAL Bünyami,ANIL BARIŞ,HALICI Mesut The Protective Effects of Osajin on Ischemia/Reperfusion Injury to Rat Ovaries: Biochemical and Histopathological Evaluation. , 2015, ss.753 - 760.
AMA	Çığşar G,keleş o,CAN S,KARACA M,Can D,Erol H,SAĞIR D,Ozgül Abuc O,ÇAKIR A,ŞAHİN Z,ÜNAL B,ANIL B,HALICI M The Protective Effects of Osajin on Ischemia/Reperfusion Injury to Rat Ovaries: Biochemical and Histopathological Evaluation. . 2015; 753 - 760.
Vancouver	Çığşar G,keleş o,CAN S,KARACA M,Can D,Erol H,SAĞIR D,Ozgül Abuc O,ÇAKIR A,ŞAHİN Z,ÜNAL B,ANIL B,HALICI M The Protective Effects of Osajin on Ischemia/Reperfusion Injury to Rat Ovaries: Biochemical and Histopathological Evaluation. . 2015; 753 - 760.
IEEE	Çığşar G,keleş o,CAN S,KARACA M,Can D,Erol H,SAĞIR D,Ozgül Abuc O,ÇAKIR A,ŞAHİN Z,ÜNAL B,ANIL B,HALICI M "The Protective Effects of Osajin on Ischemia/Reperfusion Injury to Rat Ovaries: Biochemical and Histopathological Evaluation." , ss.753 - 760, 2015.
ISNAD	Çığşar, Gülşen vd. "The Protective Effects of Osajin on Ischemia/Reperfusion Injury to Rat Ovaries: Biochemical and Histopathological Evaluation". (2015), 753-760.

APA	Çığşar G, keleş o, CAN S, KARACA M, Can D, Erol H, SAĞIR D, Ozgül Abuc O, ÇAKIR A, ŞAHİN Z, ÜNAL B, ANIL B, HALICI M (2015). The Protective Effects of Osajin on Ischemia/Reperfusion Injury to Rat Ovaries: Biochemical and Histopathological Evaluation. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 21(5), 753 - 760.
Chicago	Çığşar Gülşen,keleş osman nuri,CAN Serpil,KARACA MEHMET,Can Dr İsmail,Erol Huseyin Serkan,SAĞIR Dilek,Ozgül Abuc Ozlem,ÇAKIR AHMET,ŞAHİN Zişan Gönül,ÜNAL Bünyami,ANIL BARIŞ,HALICI Mesut The Protective Effects of Osajin on Ischemia/Reperfusion Injury to Rat Ovaries: Biochemical and Histopathological Evaluation. Kafkas Üniversitesi Veteriner Fakültesi Dergisi 21, no.5 (2015): 753 - 760.
MLA	Çığşar Gülşen,keleş osman nuri,CAN Serpil,KARACA MEHMET,Can Dr İsmail,Erol Huseyin Serkan,SAĞIR Dilek,Ozgül Abuc Ozlem,ÇAKIR AHMET,ŞAHİN Zişan Gönül,ÜNAL Bünyami,ANIL BARIŞ,HALICI Mesut The Protective Effects of Osajin on Ischemia/Reperfusion Injury to Rat Ovaries: Biochemical and Histopathological Evaluation. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, vol.21, no.5, 2015, ss.753 - 760.
AMA	Çığşar G,keleş o,CAN S,KARACA M,Can D,Erol H,SAĞIR D,Ozgül Abuc O,ÇAKIR A,ŞAHİN Z,ÜNAL B,ANIL B,HALICI M The Protective Effects of Osajin on Ischemia/Reperfusion Injury to Rat Ovaries: Biochemical and Histopathological Evaluation. Kafkas Üniversitesi Veteriner Fakültesi Dergisi. 2015; 21(5): 753 - 760.
Vancouver	Çığşar G,keleş o,CAN S,KARACA M,Can D,Erol H,SAĞIR D,Ozgül Abuc O,ÇAKIR A,ŞAHİN Z,ÜNAL B,ANIL B,HALICI M The Protective Effects of Osajin on Ischemia/Reperfusion Injury to Rat Ovaries: Biochemical and Histopathological Evaluation. Kafkas Üniversitesi Veteriner Fakültesi Dergisi. 2015; 21(5): 753 - 760.
IEEE	Çığşar G,keleş o,CAN S,KARACA M,Can D,Erol H,SAĞIR D,Ozgül Abuc O,ÇAKIR A,ŞAHİN Z,ÜNAL B,ANIL B,HALICI M "The Protective Effects of Osajin on Ischemia/Reperfusion Injury to Rat Ovaries: Biochemical and Histopathological Evaluation." Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 21, ss.753 - 760, 2015.
ISNAD	Çığşar, Gülşen vd. "The Protective Effects of Osajin on Ischemia/Reperfusion Injury to Rat Ovaries: Biochemical and Histopathological Evaluation". Kafkas Üniversitesi Veteriner Fakültesi Dergisi 21/5 (2015), 753-760.