Effects of Resveratrol on Acute Sciatic Nerve Injury in a Rat Model

kara, Mikail; AKYOL, VEYSEL; AK, Hakan; GULSEN, İsmail; RAĞBETLİ, Murat Çetin; ATALAY, Tugay

Effects of Resveratrol on Acute Sciatic Nerve Injury in a Rat Model

Hakan AK, (Bozok Üniversitesi, Tıp Fakültesi, Nöroşirürji Anabilim Dalı, Yozgat, Türkiye)

İsmail GULSEN, (Yüzüncü Yıl Üniversitesi, Tıp Fakültesi, Nöroşirürji Anabilim Dalı, Van, Türkiye)

Mikail KARA, (Yüzüncü Yıl Üniversitesi, Tıp Fakültesi, Tıbbi Histoloji ve Embriyoloji Anabilim Dalı, Van, Türkiye)

Tugay ATALAY, (Bozok Üniversitesi, Tıp Fakültesi, Nöroşirürji Anabilim Dalı, Yozgat, Türkiye)

Veysel AKYOL, (Yüzüncü Yıl Üniversitesi, Tıp Fakültesi, Tıbbi Histoloji ve Embriyoloji Anabilim Dalı, Van, Türkiye)

Murat Çetin RAĞBETLİ (Yüzüncü Yıl Üniversitesi, Tıp Fakültesi, Tıbbi Histoloji ve Embriyoloji Anabilim Dalı, Van, Türkiye)

Journal of Neurological Sciences (Turkish)

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Yıl: 2015 Cilt: 32 Sayı: 2 Sayfa Aralığı: 303 - 310 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022

Effects of Resveratrol on Acute Sciatic Nerve Injury in a Rat Model

Öz:

Amaç: Doğal bir fenol olan resveratrol'ün (RVT) deneysel sıçan modelinde akut sinir hasarıtedavisinde terapötik etkinliğini araştırmaktır.Gereç ve yöntemler: Toplamda 28 sıçan dört gruba ayrılmıştır; travma, kontrol, düşük dozRVT ve yüksek doz RVT (n=7). Kontrol grubu dışındaki diğer gruplarda orta-basınçlıanevrizma klibi ile 30 saniye boyunca sinir hasarı oluşturuldu. Travma sonrası 7 gün boyuncaRVT tedavisi verildi. Sonrasında, hasar yerinden alınan sinir dokusu örnekleri histolojik vesteriyolojik analizlere tabi tutuldu.Bulgular: Myelin kalınlığı yönünden gruplar arasında anlamlı farklılık saptandı. 50 mg/kgRVT ile tedavinin myelin kalınlığını artırdığı bununla birlikte 100 mg/kg dozunda myelinkalınlığında azalma olduğu tespit edildi.Sonuç: RVT siyatik sinir akut ezilme yaralarında 50 mg/kg dozunda faydalı etkiye sahiptir.

Anahtar Kelime:

Konular: Nörolojik Bilimler

Deneysel Sıçan Modelinde Akut Siyatik Sinir Hasarında Resveratrol'ün Etkisi

Öz:

Aim: This study evaluated the therapeutic efficacy of resveratrol (RVT), a naturally occurringpolyphenol, during the management of acute nerve injury in a rat model.Materials and Methods: A total of 28 rats were divided into trauma, control and low- andhigh-dose RVT groups (all n = 7). In all three non-control groups, trauma was induced by amedium-pressure aneurysm clip, applied to the sciatic nerve for 30 s. After trauma, theanimals were treated with RVT for 7 days, after which nerve tissue samples were obtainedfrom the site of injury for use in histological and stereological analyses.Results: There was a significant group difference in myelin thickness. At 50 mg/kg, RVTtreatment was associated with increased myelin thickness, but a decrease was observed at 100mg/kg.Conclusion: RVT has a beneficial effect on acute crush injury of the sciatic nerve at a dose of50 mg/kg.

Anahtar Kelime:

Konular: Nörolojik Bilimler

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

1. Ateş O, Çaylı S, Altınöz E, Gürses I, Yücel N, Koçak A. Eff ects of resveratrol and methylp rednisolone on biochemical, neurobehavioral and histop athological recovery af ter exp erimental sp inal cord inj ury. Acta Pharmacol Sin. 2006; 27 (10) : 1317-1325.
2. Ateş O, Çaylı S, Altınöz E, Gürses I, Yücel N, Şener M, Koçak A, Yoloğlu S. Neurop rotection by resveratrol against traumatic brain inj ury in rats. Mol. Cell Biochem. 2007;294:137-144.
3. Bağrıyanık HA, Ersoy N, Çetinkaya C, İkizoğlu E, Kutri D, Özcana T, Kamanga LG, Kıray M. The eff ects of resveratrol on chronic constriction inj ury of sciatic nerve in rats. Neurosci Lett. 2014;561:123-127. (doi: 10.1016/j .neulet.2013.12.056 ) .
4. Gao D, Zhang X, Jiang X, Peng Y, Huang W, Cheng G, Song L: Resveratrol reduces the elevated level of MMP-9 induced by cerebral ischemia-rep erf usion in mice. Lif e Sci. 2006;78:2564-2570.
5. Guneli E, A. Onal, M. Ates, H.A. Bagriyanik, H. Resmi, C.E. Orhan, H.E. Kolatan, M.Gumustekin. Eff ects of rep eated administered ghrelin on chronic constriction inj ury of the sciatic nerve in rats. Neurosci. Lett. 2010; 479: 226-230.
6. Inoue H, Jiang XF, Katayama T, Osada S, Umesono K, Namura S. Brain p rotection by resveratrol and f enof ibrate against stroke requires p eroxisome p rolif erator-activated recep tor alp ha in mice. Neurosci Lett. 2003;352:203-206.
7. Jang JH, Surh YJ. Protective eff ect of resveratrol on beta-amyloidinduced oxidative PC12 cell death. Free Radic Biol Med. 2003 Ap r 15;34(8) :1100-1110.
8. Jonsson K, Jensen A, Goodson WH, Scheuenstuhi H, West J, Hopf H W, Kunt T. Tissue oxygenation, anemia, and p erf usion in relation to wound healing in surgical p atients. Ann. Surg, 1991; 605-613.
9. Kızıltep e U, Turan NN, Han U, Ulus AT, kar F. Resveratrol, a red wine p olyphenol, p rotects sp inal cord f rom ischemia-rep erf usion inj ury. J Vasc. Surg. 2004;40(1) :138-145.
10. Liu C, Shi Z, Fan L, Zhang C, Wang K, Wang B. Resveratrol imp roves neuron p rotection and f unctional recovery in rat model of sp inal cord inj ury. Brain Res. 2011; 1374:100-109.
11. Pakkenberg B, Gundersen, HJG. Total number of neurons and glial cells in human brain nuclei estimated by the dissector and the f ractionator. J. Microsc. 1998;150: 120.
12. Raval AP, Dave KR, Perez-Pinzon MA. Resveratrol mimics ischemic p reconditioning in the brain. J. Cereb. Blood Flow Metab. 2006; 26 (9) : 11411147.
13. Rocha-Gonzalez HI, Ambriz-Tututi M, Granados-Soto V. Resveratrol: a natural comp ound with p harmacological p otential in neurodegenerative diseases. CNS Neurosci Ther. 2008;14(3) :234-247.
14. Sönmez Ü, Sönmez A, Erbil G, Tekmen I, Baykara B. Neurop rotective eff ects of resveratrol against traumatic brain inj ury in immature rats. Neurosci Lett. 2007;420(2) :133- 137.
15. Şenoglu M, Nacitarhan V, Kurutas EB, Şenoğlu N, Altun İ, Atli Y, Özbağ D. Intrap eritoneal Alp ha-Lip oic Acid to p revent neural damage af ter crush inj ury to the rat sciatic nerve. Journal of Brachial Plexus and Perip heral Nerve Inj ury 2009; 4:22 (doi:10.1186/1749- 7221-4-22.) .
16. Tsai SK, Hung LM, Fu YT, Cheng H, Nien M W, Liu HY, Zhang FB, Huang SS. Resveratrol neurop rotective eff ects during f ocal cerebral ischemia inj ury via nitric oxide mechanism in rats. J Vasc Surg 2007;46:346353.
17. Turgut M, Kap lan S, Ünal BZ, Bozkurt M, Yürüker S, Yenisey Ç, Sahin B, Uyanıkgil Y, Baka M. Stereological analysis of sciatic nerve in chickens f ollowing neonatal p inealectomy : an exp erimental study. Journal of Brachial Plexus and Perip heral Nerve Inj ury 2010, 5:10.
18. Woods AE, Stirling J W: Electron microscopy. In Theory and Pratice of Histological Techniques Edited by : Bancrof t JD, Gamble M. China: Churchill Livingstone Elsevier; 2008- 611.
19. Yalçın SS, Ak H, Aydın V, Serter M, Göçmen A Y. Evaluation of the acute p eriod eff ects of nicergoline in the management of acute p erip heral nerve inj ury. J Neurol Sci Turk. 2013;30(4) :651-659.
20. Yılmaz Z, Senoglu M, Kurutas EB, Ciralik H, Ozbağ D. Neurop rotective Eff ects of Mannitol And Vitamin C on Crush Inj ury of Sciatic Nerve. An Exp erimental Rat Study. J Neurol Sci Turk. 2011; 28:(4) : 538-551.
21. Zamin LL, Dillenburg-Pilla P, Argenta- Comiran R, Horn AP, Simao F, Nassif M, Gerhardt D, Frozza RL, Salbego C. Protective eff ect of resveratrol against oxygen-glucose dep rivation in organotyp ic hipp ocamp al slice cultures: involvement of PI3-K p athway. Neurobiol. Dis. 2006;24 (1) : 170182.
22. Zhang F, Liu J, Shi J-S. Anti-inf lammatory activities of resveratrol in the brain: role of resveratrol in microglial activation. Eur J Pharmacol. 2010; 636(1-3) :1-7.
23. Zhuang H, Kim YS, Koehler RC, Dore S. Potential mechanism by which resveratrol, a red wine constituent, p rotects neurons. Ann N Y Acad Sci. 2003; 993:276-286.

APA	AK H, GULSEN İ, kara M, ATALAY T, AKYOL V, RAĞBETLİ M (2015). Effects of Resveratrol on Acute Sciatic Nerve Injury in a Rat Model. , 303 - 310.
Chicago	AK Hakan,GULSEN İsmail,kara Mikail,ATALAY Tugay,AKYOL VEYSEL,RAĞBETLİ Murat Çetin Effects of Resveratrol on Acute Sciatic Nerve Injury in a Rat Model. (2015): 303 - 310.
MLA	AK Hakan,GULSEN İsmail,kara Mikail,ATALAY Tugay,AKYOL VEYSEL,RAĞBETLİ Murat Çetin Effects of Resveratrol on Acute Sciatic Nerve Injury in a Rat Model. , 2015, ss.303 - 310.
AMA	AK H,GULSEN İ,kara M,ATALAY T,AKYOL V,RAĞBETLİ M Effects of Resveratrol on Acute Sciatic Nerve Injury in a Rat Model. . 2015; 303 - 310.
Vancouver	AK H,GULSEN İ,kara M,ATALAY T,AKYOL V,RAĞBETLİ M Effects of Resveratrol on Acute Sciatic Nerve Injury in a Rat Model. . 2015; 303 - 310.
IEEE	AK H,GULSEN İ,kara M,ATALAY T,AKYOL V,RAĞBETLİ M "Effects of Resveratrol on Acute Sciatic Nerve Injury in a Rat Model." , ss.303 - 310, 2015.
ISNAD	AK, Hakan vd. "Effects of Resveratrol on Acute Sciatic Nerve Injury in a Rat Model". (2015), 303-310.

APA	AK H, GULSEN İ, kara M, ATALAY T, AKYOL V, RAĞBETLİ M (2015). Effects of Resveratrol on Acute Sciatic Nerve Injury in a Rat Model. Journal of Neurological Sciences (Turkish), 32(2), 303 - 310.
Chicago	AK Hakan,GULSEN İsmail,kara Mikail,ATALAY Tugay,AKYOL VEYSEL,RAĞBETLİ Murat Çetin Effects of Resveratrol on Acute Sciatic Nerve Injury in a Rat Model. Journal of Neurological Sciences (Turkish) 32, no.2 (2015): 303 - 310.
MLA	AK Hakan,GULSEN İsmail,kara Mikail,ATALAY Tugay,AKYOL VEYSEL,RAĞBETLİ Murat Çetin Effects of Resveratrol on Acute Sciatic Nerve Injury in a Rat Model. Journal of Neurological Sciences (Turkish), vol.32, no.2, 2015, ss.303 - 310.
AMA	AK H,GULSEN İ,kara M,ATALAY T,AKYOL V,RAĞBETLİ M Effects of Resveratrol on Acute Sciatic Nerve Injury in a Rat Model. Journal of Neurological Sciences (Turkish). 2015; 32(2): 303 - 310.
Vancouver	AK H,GULSEN İ,kara M,ATALAY T,AKYOL V,RAĞBETLİ M Effects of Resveratrol on Acute Sciatic Nerve Injury in a Rat Model. Journal of Neurological Sciences (Turkish). 2015; 32(2): 303 - 310.
IEEE	AK H,GULSEN İ,kara M,ATALAY T,AKYOL V,RAĞBETLİ M "Effects of Resveratrol on Acute Sciatic Nerve Injury in a Rat Model." Journal of Neurological Sciences (Turkish), 32, ss.303 - 310, 2015.
ISNAD	AK, Hakan vd. "Effects of Resveratrol on Acute Sciatic Nerve Injury in a Rat Model". Journal of Neurological Sciences (Turkish) 32/2 (2015), 303-310.