Serum interleukin-6, interleukin -10, tumor necrosis factor -alpha, neopterin and plasma soluble urokinase plasminogen activator receptor levels in the patients with pandemic H1N1 influenza infection

FİDAN, Yasemin; ÖZDAMAR, Sema; DEMİRÖZ, Ali Pekcan; IRMAK, HASAN; Cesur, Salih; KARABULUT, Erdem; Akdeniz, Hayrettin; KARAHOCAGİL, Mustafa Kasım

Serum interleukin-6, interleukin -10, tumor necrosis factor -alpha, neopterin and plasma soluble urokinase plasminogen activator receptor levels in the patients with pandemic H1N1 influenza infection

Hasan IRMAK, (Ankara Eğitim ve Araştırma Hastanesi, Enfeksiyon Hastalıkları ve Klinik Mikrobiyoloji Kliniği, Ankara, Türkiye)

Mustafa Kasım KARAHOCAGİL, (Yüzüncü Yıl Üniversitesi, Tıp Fakültesi, Enfeksiyon Hastalıkları ve Klinik Mİkrobiyoloji Anabilim Dalı, Van, Türkiye)

Salih CESUR, (Ankara Eğitim ve Araştırma Hastanesi, Enfeksiyon Hastalıkları ve Klinik Mikrobiyoloji Kliniği, Ankara, Türkiye)

Yasemin FİDAN, (Ankara Eğitim ve Araştırma Hastanesi, Biyokimya ve Klinik Biyokimya Kliniği, Ankara, Türkiye)

Sema ÖZDAMAR, (Ankara Eğitim ve Araştırma Hastanesi, Biyokimya ve Klinik Biyokimya Kliniği, Ankara, Türkiye)

Erdem KARABULUT, (Hacettepe Üniversitesi, Tıp Fakültesi, Biyokimya Anabilim Dalı, Ankara, Türkiye)

Hayrettin AKDENİZ, (Yüzüncü Yıl Üniversitesi, Tıp Fakültesi, Enfeksiyon Hastalıkları ve Klinik Mİkrobiyoloji Anabilim Dalı, Van, Türkiye)

Ali Pekcan DEMİRÖZ (Ankara Eğitim ve Araştırma Hastanesi, Enfeksiyon Hastalıkları ve Klinik Mikrobiyoloji Kliniği, Ankara, Türkiye)

Journal of Microbiology and Infectious Diseases

2 0

Yıl: 2015 Cilt: 5 Sayı: 03 Sayfa Aralığı: 119 - 124 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022

Serum interleukin-6, interleukin -10, tumor necrosis factor -alpha, neopterin and plasma soluble urokinase plasminogen activator receptor levels in the patients with pandemic H1N1 influenza infection

Öz:

Amaç: Bu çalışmada, klinik ve laboratuvar bulgularıyla kesin pandemic influenza A (H1N1) enfeksiyonu tanısı konan yatan hastalar, üst solunum yolu enfeksiyonu enfeksiyonu tanısı konan poliklinik hastlarında serum IL-6, IL-10, TNF-alfa, neopterin düzeyleri ve plazma solubl ürokinaz plazminojen aktivatör reseptörü (suPAR) düzeyinin klinik öneminin belirlenmesi amaçlandı. Yöntem: Çalışmaya influenza A (H1N1) enfeksiyonu tanısı klinik ve laboratuvar testiyle (polimeraz zincir reaksiyonu: PZR) tanısı konan 26 yatan hasta, üst solunum yolu enfeksiyonu (akut sinüzit, akut tonsillofarenjit, akut nazofarenjit vb.)tanısı konan 30 poliklinik hastası ve hiçbir yakınması olmayan 24 sağlıklı birey dahil edildi. Serum IL-6, IL-10, TNF-alfa, neopterin düzeyleri ve plazma suPAR düzeyi ELISA yöntemiyle üretici firmanın önerileri doğrultusunda belirlendi. İstatistiksel değerlendirmede Kruskal- Wallis, Mann-Whitney U testi ve Ki-kare testleri kullanıldı.Bulgular: İnfluenza A (H1N1) enfeksiyonu olan yatan hastalarda serum IL-6, IL-10, TNF-alfa, neopterin düzeyleri ile plazma suPAR düzeyleri median değeri sırasıyla; 27,8 ng/ml, 9,29 ng/ml, 11,04 ng/ml, 8,82 ng/ml ,5,64 ng/ml olarak belirlendi.Üst solunum yolu enfeksiyonu tanısı konan poliklinik hastalarında serum IL-6, IL-10, TNF-alfa, neopterin düzeyleri ve plazma suPAR düzeyi median (ortanca) değeri sırasıyla; 7,24 ng/ml, 1,9 ng/ml, 19,74 ng/ml, 4,37 ng/ml, 3,33 ng/ml idi.Sağlıklı bireylerde ise serum IL-6, IL-10, TNF-alfa, neopterin düzeyleri ve plazma suPAR düzeyi median değeri sırasıyla; 0,97 ng/ml, 0,16 ng/ml, 4,46 ng/ml, 2,38 ng/ml, 1,65 ng/ml olarak belirlendi.İnfluenza A (H1N1) enfeksiyonu tanısı konan yatan hastalarda serum IL-6, IL-10, neopterin düzeyleri, üst solunum yolu enfeksiyonu tanısı konan poliklinik hastalarınkınden istatistiksel olarak anlamlı yüksekti. Üst solunum yolu enfeksiyonu tanısı konan poliklinik hastalarında serum TNF-alfa düzeyleri H1N1 tanısı konan yatan hastalardan daha yüksekti. Üst solunum yolu enfeksiyonu tanısı konan poliklinik hastalarında, serum IL-6, IL-10, TNF-alfa, neopterin düzeyleri ile plazma suPAR düzeyleri kontrol grubundan anlamlı oranda yüksekti.İnfluenza A (H1N1) enfeksiyonu tanısı alan yatan hastalarda serum IL-6, IL-10,neopterin ve plazma suPAR düzeyi sağlıklı bireylere göre anlamlı yükseklik gösterdi.Sonuç: Serum IL-6, IL-10, neopterin, TNF-alfa düzeyleri ve plazma suPAR düzeyi influenza A (H1N1) enfeksiyonu olan hastaların klinik izleminde kullanılabilir

Anahtar Kelime:

Konular: Mikrobiyoloji Enfeksiyon Hastalıkları

Pandemik İnfluenza A (H1N1) Enfeksiyonu Olan Hastalarda Serum IL-6, IL-10, TNF-alfa, neopterin ve plazma solubl ürokinaz plasminojen aktivatör reseptörü düzeylerinin klinik önemi

Öz:

Objective: This study aims to investigate the clinical importance of serum interleukin (IL) 6, IL-10, tumor necrosis factor alpha (TNF-alpha), neopterin, and plasma soluble urokinase plasminogen activator receptor (suPAR) levels in patients with pandemic H1N1 influenza infection and outpatients diagnosed with upper respiratory infections.Materials and Methods: Twenty-six inpatients with H1N1 infection confirmed by clinical findings and polymerase chain reaction (PCR) test 30 outpatients diagnosed with upper respiratory infections, and 24 healthy individuals without any complaints were included. Serum IL-6, IL-10, TNF-alpha, neopterin levels and plasma suPAR levels were compared between the patients groups and the 24 healthy controls. Serum IL-6, IL-10, TNF-alpha, neopterin levels and plasma suPAR levels were determined through the enzyme-linked immunosorbent assay (ELISA) as per manufacturer's instructions. Results: The median serum IL-6, IL-10, TNF-alpha, neopterin, and plasma suPAR levels were 27.8 ng/ml, 9.29 ng/ml, 11.04 ng/ml, 8.82 ng/ml and 5.64 ng/ml, respectively in inpatients with H1N1. In 30 outpatients diagnosed with upper respiratory infections, the median serum IL-6, IL-10, TNF-alpha, neopterin, and plasma suPAR levels were 7.24 ng/ml, 1.9 ng/ml, 19.74 ng/ml, 4.37ng/ml and 3.33 ng/ml, respectively.In healthy individuals, the mean IL-6, IL-10, TNF-alpha, neopterin, and plasma suPAR levels were 97 ng/ml, 0.16 ng/ml, 4.46 ng/ml, 2.38 ng/ml, 1.65 ng/ml, respectively.In inpatients with H1N1, serum IL-6, IL-10, neopterin levels were significantly higher than those of outpatients diagnosed with upper respiratory infections. In outpatients with upper respiratory infections, serum TNF-alpha levels were higher than in inpatients with H1N1 infection as confirmed by laboratory findings (PCR).In outpatients, serum IL-6, IL-10, TNF-alpha, neopterin, and plasma suPAR levels were significantly higher from those in the control group. In patients with influenza A (H1N1) infection, serum cytokine levels and plasma suPAR levels were significantly higher from healthy individuals.Conclusion: Serum IL-6, IL-10, TNF-alpha, neopterin, and plasma suPAR levels may be used as clinical follow-up markers in patients with H1N1 infection. J Microbiol Infect Dis 2015;5(3): 119-124

Anahtar Kelime:

Konular: Mikrobiyoloji Enfeksiyon Hastalıkları

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

Lee N, Wong CK, Chan PK, et al. Cytokine response patterns in severe pandemic 2009 H1N1 and seasonal influenza among hospitalized adults. PLoS One. 2011;6:e26050.
World Health Organization. Influenza, Available http://www. who.int/topics/ influenza/en. Accessed 2011 May, 1.
Shaw M, Uyeki TM, Zaki SR, et al. Clinical aspects of pan- demic 2009 influenza A (H1N1) virus infection. N Engl J Med 2010; 362:1708-1719.
TC Sağlık Bakanlığı, Refik Saydam Hıfzıssıhha Merkezi Baş- kanlığı. Türkiye İnfluenza Sürveyans Raporu 2010-2011. (Turkish)
Maral I, Eskiocak M, Öner A. Hasuder Sağlık Raporu, 2012. (Turkish)
Guo X, Chen Y, Li X, et al. Dynamic variations in the periph- eral blood lymphocyte subgroups of patients with 2009 pan- demic H1N1 swine-origin influenza A virus infection. Virol J 2011;8:215.
Liu Y, Chen H, Sun Y, Chen F. Antiviral role of toll-like receptors and cytokines against the new 2009 H1N1 virus infection. Mol Biol Rep 2012;39:1163-1172.
Hagau N, Slavcovici A, Gonganau DN, et al. Clinical aspects and cytokine response in severe H1N1 influenza A virus in- fection. Crit Care 2010;14:R203.
Yu X, Zhang X, Zhao B, et al. Intensive cytokine induction in pandemic H1N1 influenza virus infection accompanied by ro- bust production of IL-10 and IL-6. PLoS One 2011;6:e28680.
Paquette SG, Banner D, Zhao Z, et al. Interleukin-6 Is a Po- tential Biomarker for Severe Pandemic H1N1 Influenza A In- fection. PLoS One 2012;7:e38214.
Almansa R, Anton A, Ramirez P, et al. Direct association between pharyngeal viral secretion and host cytokine re- sponse in severe pandemic influenza. BMC Infect Dis 2011;31;11:232.
Jost S, Quillay H, Reardon J, Peterson E. Changes in cyto- kine levels and NK cell activation associated with influenza. PLoS One 2011;6:e25060.
Shen HH, Hou J, Chen WW, et al. Immunologic changes dur- ing Pandemic (H1N1) 2009, China. Emerg Infect Dis. 2011; 17:1053-1055.
Hayden FG, Fritz R, Lobo MC, et al. Local and systemic cyto- kine responses during experimental human influenza A virus infection. Relation to symptom formation and host defense. J Clin Invest 1998; 101:643-649.
Kaiser L, Fritz RS, Straus SE, et al. Symptom pathogenesis during acute influenza: interleukin-6 and other cytokine re- sponses. J Med Virol 2001;64:262-268.
Mukherjee S, Vipat VC, Mishra AC, et al. Pandemic (H1N1) 2009 influenza virus induces weaker host immune respons- es in vitro: a possible mechanism of high transmissibility. Vi- rol J 2011; 8:140.
Kim YH, Kim JE, Hyun MC. Cytokine response in pediatric patients with pandemic influenza H1N1 2009 virus infection and pneumonia: comparison with pediatric pneumonia with- out H1N1 2009 infection. Pediatr Pulmonol 2011; 46:1233- 1239.

APA	IRMAK H, KARAHOCAGİL M, Cesur S, FİDAN Y, ÖZDAMAR S, KARABULUT E, Akdeniz H, DEMİRÖZ A (2015). Serum interleukin-6, interleukin -10, tumor necrosis factor -alpha, neopterin and plasma soluble urokinase plasminogen activator receptor levels in the patients with pandemic H1N1 influenza infection. , 119 - 124.
Chicago	IRMAK HASAN,KARAHOCAGİL Mustafa Kasım,Cesur Salih,FİDAN Yasemin,ÖZDAMAR Sema,KARABULUT Erdem,Akdeniz Hayrettin,DEMİRÖZ Ali Pekcan Serum interleukin-6, interleukin -10, tumor necrosis factor -alpha, neopterin and plasma soluble urokinase plasminogen activator receptor levels in the patients with pandemic H1N1 influenza infection. (2015): 119 - 124.
MLA	IRMAK HASAN,KARAHOCAGİL Mustafa Kasım,Cesur Salih,FİDAN Yasemin,ÖZDAMAR Sema,KARABULUT Erdem,Akdeniz Hayrettin,DEMİRÖZ Ali Pekcan Serum interleukin-6, interleukin -10, tumor necrosis factor -alpha, neopterin and plasma soluble urokinase plasminogen activator receptor levels in the patients with pandemic H1N1 influenza infection. , 2015, ss.119 - 124.
AMA	IRMAK H,KARAHOCAGİL M,Cesur S,FİDAN Y,ÖZDAMAR S,KARABULUT E,Akdeniz H,DEMİRÖZ A Serum interleukin-6, interleukin -10, tumor necrosis factor -alpha, neopterin and plasma soluble urokinase plasminogen activator receptor levels in the patients with pandemic H1N1 influenza infection. . 2015; 119 - 124.
Vancouver	IRMAK H,KARAHOCAGİL M,Cesur S,FİDAN Y,ÖZDAMAR S,KARABULUT E,Akdeniz H,DEMİRÖZ A Serum interleukin-6, interleukin -10, tumor necrosis factor -alpha, neopterin and plasma soluble urokinase plasminogen activator receptor levels in the patients with pandemic H1N1 influenza infection. . 2015; 119 - 124.
IEEE	IRMAK H,KARAHOCAGİL M,Cesur S,FİDAN Y,ÖZDAMAR S,KARABULUT E,Akdeniz H,DEMİRÖZ A "Serum interleukin-6, interleukin -10, tumor necrosis factor -alpha, neopterin and plasma soluble urokinase plasminogen activator receptor levels in the patients with pandemic H1N1 influenza infection." , ss.119 - 124, 2015.
ISNAD	IRMAK, HASAN vd. "Serum interleukin-6, interleukin -10, tumor necrosis factor -alpha, neopterin and plasma soluble urokinase plasminogen activator receptor levels in the patients with pandemic H1N1 influenza infection". (2015), 119-124.

APA	IRMAK H, KARAHOCAGİL M, Cesur S, FİDAN Y, ÖZDAMAR S, KARABULUT E, Akdeniz H, DEMİRÖZ A (2015). Serum interleukin-6, interleukin -10, tumor necrosis factor -alpha, neopterin and plasma soluble urokinase plasminogen activator receptor levels in the patients with pandemic H1N1 influenza infection. Journal of Microbiology and Infectious Diseases, 5(03), 119 - 124.
Chicago	IRMAK HASAN,KARAHOCAGİL Mustafa Kasım,Cesur Salih,FİDAN Yasemin,ÖZDAMAR Sema,KARABULUT Erdem,Akdeniz Hayrettin,DEMİRÖZ Ali Pekcan Serum interleukin-6, interleukin -10, tumor necrosis factor -alpha, neopterin and plasma soluble urokinase plasminogen activator receptor levels in the patients with pandemic H1N1 influenza infection. Journal of Microbiology and Infectious Diseases 5, no.03 (2015): 119 - 124.
MLA	IRMAK HASAN,KARAHOCAGİL Mustafa Kasım,Cesur Salih,FİDAN Yasemin,ÖZDAMAR Sema,KARABULUT Erdem,Akdeniz Hayrettin,DEMİRÖZ Ali Pekcan Serum interleukin-6, interleukin -10, tumor necrosis factor -alpha, neopterin and plasma soluble urokinase plasminogen activator receptor levels in the patients with pandemic H1N1 influenza infection. Journal of Microbiology and Infectious Diseases, vol.5, no.03, 2015, ss.119 - 124.
AMA	IRMAK H,KARAHOCAGİL M,Cesur S,FİDAN Y,ÖZDAMAR S,KARABULUT E,Akdeniz H,DEMİRÖZ A Serum interleukin-6, interleukin -10, tumor necrosis factor -alpha, neopterin and plasma soluble urokinase plasminogen activator receptor levels in the patients with pandemic H1N1 influenza infection. Journal of Microbiology and Infectious Diseases. 2015; 5(03): 119 - 124.
Vancouver	IRMAK H,KARAHOCAGİL M,Cesur S,FİDAN Y,ÖZDAMAR S,KARABULUT E,Akdeniz H,DEMİRÖZ A Serum interleukin-6, interleukin -10, tumor necrosis factor -alpha, neopterin and plasma soluble urokinase plasminogen activator receptor levels in the patients with pandemic H1N1 influenza infection. Journal of Microbiology and Infectious Diseases. 2015; 5(03): 119 - 124.
IEEE	IRMAK H,KARAHOCAGİL M,Cesur S,FİDAN Y,ÖZDAMAR S,KARABULUT E,Akdeniz H,DEMİRÖZ A "Serum interleukin-6, interleukin -10, tumor necrosis factor -alpha, neopterin and plasma soluble urokinase plasminogen activator receptor levels in the patients with pandemic H1N1 influenza infection." Journal of Microbiology and Infectious Diseases, 5, ss.119 - 124, 2015.
ISNAD	IRMAK, HASAN vd. "Serum interleukin-6, interleukin -10, tumor necrosis factor -alpha, neopterin and plasma soluble urokinase plasminogen activator receptor levels in the patients with pandemic H1N1 influenza infection". Journal of Microbiology and Infectious Diseases 5/03 (2015), 119-124.