The effects of caffeic acid phenethyl ester on inflammatory cytokines after acute spinal cord injury

ÇELİKBİLEK, Asuman; YILMAZ, Tevfik; KOÇAK, Havva; Demir, İsmail; CANDAN, Aydın; KARAASLAN, Tamer; ALACA, İlker; ATALAY, Tugay; AK, Hakan; GULSEN, İsmail

The effects of caffeic acid phenethyl ester on inflammatory cytokines after acute spinal cord injury

Hakan AK, (Bozok Üniversitesi, Tıp Fakültesi, Nöroşirürji Anabilim Dalı, Yozgat, Türkiye)

İsmail GULSEN, (Yüzüncü Yıl Üniversitesi, Tıp Fakültesi, Nöroşirürji Anabilim Dalı, Van, Türkiye)

Tamer KARAASLAN, (Süleyman Demirel Üniversitesi, Tıp Fakültesi, Nöroşirürji Anabilim Dalı, Isparta, Türkiye)

İlker ALACA, (Süleyman Demirel Üniversitesi, Tıp Fakültesi, Nöroşirürji Anabilim Dalı, Isparta, Türkiye)

Aydın CANDAN, (Süleyman Demirel Üniversitesi, Tıp Fakültesi, Histoloji ve Embriyoloji Anabilim Dalı, Isparta, Türkiye)

Havva KOÇAK, (Dumlupınar Üniversitesi, Tıp Fakültesi, Biyokimya Anabilim Dalı, Kütahya, Türkiye)

Tugay ATALAY, (Bozok Üniversitesi, Tıp Fakültesi, Nöroşirürji Anabilim Dalı, Yozgat, Türkiye)

Asuman ÇELİKBİLEK, (Bozok Üniversitesi, Tıp Fakültesi, Nöroloji Anabilim Dalı, Yozgat, Türkiye)

İsmail DEMİR, (Sağlık Bakanlığı, Van Bölge Eğitim ve Araştırma Hastanesi, Nöroşirürji Kliniği, Van, Türkiye)

Tevfik YILMAZ (Dicle Üniversitesi, Tıp Fakültesi, Nöroşirürji Anabilim Dalı, Diyarbakır, Türkiye)

Ulusal Travma ve Acil Cerrahi Dergisi

1 0

Yıl: 2015 Cilt: 21 Sayı: 2 Sayfa Aralığı: 96 - 101 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022

The effects of caffeic acid phenethyl ester on inflammatory cytokines after acute spinal cord injury

Öz:

AMAÇ: Bu çalışmada, akut spinal kord hasarı sonrası erken dönemde kafeik asit fenetil esterin (KAFE) enflamatuvar sitokinlerden interlökin 1 beta (IL-1?) ve tümör nekrotizan faktor alfa (TNF-?) üzerine etkisini ve histopatolojik olarak KAFE'nin olası iyileştirici etkisini araştırmak amaçlandı.GEREÇ VE YÖNTEM: Çalışmada ağırlıkları 250-300 gram arasında değişen 48 Wistar-Albino cinsi sıçan kullanıldı. Denekler üç gruba ayrıldı. Her grup kendi altında iki alt gruba ayrıldı. 1a grubu kontrol grubu olup bu grupta yalnızca laminektomi yapıldı. Grup 1b'de laminektomi sonrası anevriz-ma klibi ile travma oluşturuldu. İkinci gruptaki deneklerde travma oluşturulduktan yarım saat sonra serum fizyolojik sistemik olarak verilip birinci ve altıncı saatte denekler sakrifiye edildi. Üçüncü grupta travma oluşturulmuş deneklere yarım saat sonra sistemik yoldan KAFE verildi ve bu denekler birinci ve altıncı saatte sakrifiye edildi. Sakrifikasyon öncesi kalpten alınan kanda ELİSA kitleri ile serum IL-1? ve TNF-? düzeyleri ölçüldü. Hasarlan-mış kordan alınan doku örneklerinde histopatolojik değerlendirme yapıldı.BULGULAR: Kafeik asit fenetil esterin verilen grupta TNF-? ve IL-1? düzeylerinin azaldığı tespit edildi. Histopatolojik değerlendirmede KAFE verilen grupta hemoraji ve nekroz oranında azalma tespit edildi.TARTIŞMA: Akut spinal kord hasarı sonrası erken dönemde KAFE enflamatuvar sitokinlerden TNF-? ve IL-1? düzeylerini baskılamaktadır ve hasar sonrası iyileşmeye katkıda bulunmaktadır.

Anahtar Kelime:

Konular: Cerrahi

Kafeik asit fenetil esterin akut spinal kord hasarı sonrasındaenflamatuvar sitokinler üzerine etkisi

Öz:

BACKGROUND: The purpose of this study was to investigate the effects of Caffeic Acid Phenethyl Ester (CAPE) on proinflammatory cytokines, IL-1? and TNF-?, and explore its healing effect after acute spinal cord injury. METHODS: Forty-eight male Wistar-Albino rats were used in this study which was planned as three groups. All groups were divided into two sub-groups. Group 1a was the control group, in which only lower segment thoracic laminectomy was performed. In group 1b, spinal cord trauma was performed with aneurysm clip. In the second group, serum physiologic was given systemically thirty minutes after trauma, and rats were sacrificed after the first and sixth hour. In the third group, CAPE was given systemically thirty minutes after trauma, and rats were sacrificed after the first and sixth hour. Serum IL-1? and TNF-? levels were analyzed by ELISA in the serum. Histopathological analysis was performed in damaged cord tissues.RESULTS: CAPE suppressed TNF-? and IL-1? levels in the serum. In histopathological evaluation, it was detected that CAPE decreased hemorrhage and necrosis.CONCLUSION: CAPE suppresses the levels of proinflammatory cytokines, TNF-? and IL-1?, after acute spinal cord injury in the early phase and contributes to the healing process

Anahtar Kelime:

Konular: Cerrahi

Belge Türü: Makale Makale Türü: Olgu Sunumu Erişim Türü: Erişime Açık

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APA	AK H, GULSEN İ, KARAASLAN T, ALACA İ, CANDAN A, KOÇAK H, ATALAY T, ÇELİKBİLEK A, Demir İ, YILMAZ T (2015). The effects of caffeic acid phenethyl ester on inflammatory cytokines after acute spinal cord injury. , 96 - 101.
Chicago	AK Hakan,GULSEN İsmail,KARAASLAN Tamer,ALACA İlker,CANDAN Aydın,KOÇAK Havva,ATALAY Tugay,ÇELİKBİLEK Asuman,Demir İsmail,YILMAZ Tevfik The effects of caffeic acid phenethyl ester on inflammatory cytokines after acute spinal cord injury. (2015): 96 - 101.
MLA	AK Hakan,GULSEN İsmail,KARAASLAN Tamer,ALACA İlker,CANDAN Aydın,KOÇAK Havva,ATALAY Tugay,ÇELİKBİLEK Asuman,Demir İsmail,YILMAZ Tevfik The effects of caffeic acid phenethyl ester on inflammatory cytokines after acute spinal cord injury. , 2015, ss.96 - 101.
AMA	AK H,GULSEN İ,KARAASLAN T,ALACA İ,CANDAN A,KOÇAK H,ATALAY T,ÇELİKBİLEK A,Demir İ,YILMAZ T The effects of caffeic acid phenethyl ester on inflammatory cytokines after acute spinal cord injury. . 2015; 96 - 101.
Vancouver	AK H,GULSEN İ,KARAASLAN T,ALACA İ,CANDAN A,KOÇAK H,ATALAY T,ÇELİKBİLEK A,Demir İ,YILMAZ T The effects of caffeic acid phenethyl ester on inflammatory cytokines after acute spinal cord injury. . 2015; 96 - 101.
IEEE	AK H,GULSEN İ,KARAASLAN T,ALACA İ,CANDAN A,KOÇAK H,ATALAY T,ÇELİKBİLEK A,Demir İ,YILMAZ T "The effects of caffeic acid phenethyl ester on inflammatory cytokines after acute spinal cord injury." , ss.96 - 101, 2015.
ISNAD	AK, Hakan vd. "The effects of caffeic acid phenethyl ester on inflammatory cytokines after acute spinal cord injury". (2015), 96-101.

APA	AK H, GULSEN İ, KARAASLAN T, ALACA İ, CANDAN A, KOÇAK H, ATALAY T, ÇELİKBİLEK A, Demir İ, YILMAZ T (2015). The effects of caffeic acid phenethyl ester on inflammatory cytokines after acute spinal cord injury. Ulusal Travma ve Acil Cerrahi Dergisi, 21(2), 96 - 101.
Chicago	AK Hakan,GULSEN İsmail,KARAASLAN Tamer,ALACA İlker,CANDAN Aydın,KOÇAK Havva,ATALAY Tugay,ÇELİKBİLEK Asuman,Demir İsmail,YILMAZ Tevfik The effects of caffeic acid phenethyl ester on inflammatory cytokines after acute spinal cord injury. Ulusal Travma ve Acil Cerrahi Dergisi 21, no.2 (2015): 96 - 101.
MLA	AK Hakan,GULSEN İsmail,KARAASLAN Tamer,ALACA İlker,CANDAN Aydın,KOÇAK Havva,ATALAY Tugay,ÇELİKBİLEK Asuman,Demir İsmail,YILMAZ Tevfik The effects of caffeic acid phenethyl ester on inflammatory cytokines after acute spinal cord injury. Ulusal Travma ve Acil Cerrahi Dergisi, vol.21, no.2, 2015, ss.96 - 101.
AMA	AK H,GULSEN İ,KARAASLAN T,ALACA İ,CANDAN A,KOÇAK H,ATALAY T,ÇELİKBİLEK A,Demir İ,YILMAZ T The effects of caffeic acid phenethyl ester on inflammatory cytokines after acute spinal cord injury. Ulusal Travma ve Acil Cerrahi Dergisi. 2015; 21(2): 96 - 101.
Vancouver	AK H,GULSEN İ,KARAASLAN T,ALACA İ,CANDAN A,KOÇAK H,ATALAY T,ÇELİKBİLEK A,Demir İ,YILMAZ T The effects of caffeic acid phenethyl ester on inflammatory cytokines after acute spinal cord injury. Ulusal Travma ve Acil Cerrahi Dergisi. 2015; 21(2): 96 - 101.
IEEE	AK H,GULSEN İ,KARAASLAN T,ALACA İ,CANDAN A,KOÇAK H,ATALAY T,ÇELİKBİLEK A,Demir İ,YILMAZ T "The effects of caffeic acid phenethyl ester on inflammatory cytokines after acute spinal cord injury." Ulusal Travma ve Acil Cerrahi Dergisi, 21, ss.96 - 101, 2015.
ISNAD	AK, Hakan vd. "The effects of caffeic acid phenethyl ester on inflammatory cytokines after acute spinal cord injury". Ulusal Travma ve Acil Cerrahi Dergisi 21/2 (2015), 96-101.