This study was aimed out to evaluate the effects of vitamin C premedication on xylazine-ketamine anesthesia in New Zealand white rabbits. Twelve New Zealand white rabbits were divided into two groups (Group XK and Group VCXK) that each had 3 males and 3 females. The animals in group XK were treated with 10 mg/kg Xylazine HCl and 50 mg/kg Ketamine HCl. The animals in group VCXK were pre-treated with 60 mg/kg Vitamin C prior to xylazine-ketamine combination. The onset of anesthesia, the length of surgical anesthesia and the recovery time were recorded. Body temperature, heart and respiratory rates were recorded at 0, 5, 10, 15, 30, 45, 60 min. In XK group onset of action and length of surgical anesthesia were 5.81±0.19 min and 55.00±2.81 min, respectively. Premedication of rabbits with 60 mg/kg vitamin C resulted in significant decrease in the onset of action (3.93±0.13 min) and increase in the duration of xylazine-ketamine anesthesia (77.50±6.15 min). The body temperature change between treatment groups were significant at 30, 45 and 60 min. This study shows that vitamin C administration prior to xylazine-ketamine anesthesia in New Zealand white rabbits decreases the time for onset of action and prolonged the length of surgical anesthesia