Comparative Analysis of Aflatoxin M1 in Marketed Butter by ELISA and HPLC
Yıl: 2016 Cilt: 22 Sayı: 4 Sayfa Aralığı: 619 - 621 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022
Comparative Analysis of Aflatoxin M1 in Marketed Butter by ELISA and HPLC
Öz: Bu çalışmada Karadeniz bölgesinde tüketime sunulan tereyağlarında iki farklı analitik yöntem karşılaştırarak aflatoksin M1 varlığının araştırılması amaçlandı. Bu amaçla, toplam 40 tereyağı numunesi ELISA testi ile tarandı ve ardından doğrulayıcı bir yöntem olannumunelerin temizleme aşamasında immunoafinite kolonların kullanıldığı floresan detektörlü yüksek performanslı sıvı kromatografi (IAC/HPLC-FLD) ile analizleri yapıldı. Yapılan çalışma ile herhangi bir tereyağı numunesinde AFM1 tespit edilmemiş olup, Karadeniz bölgesinde üretilen tereyağlarının AFM1 açısından iyi kalitede olduğu sonucuna varıldı. Ayrıca, elde edilen sonuçlara göre tereyağında AFM1 tespitinde kullanılan IAC/HPLC-FLD metodunun doğrulayıcı ve hassas bir yöntem olduğu ortaya konulmuştur.
Anahtar Kelime: Konular:
Tereyağlarında Aflatoksin M1 düzeylerinin ELISA ve HPLC ile Karşılaştırmalı Analizi
Öz: The aim of this study was to examine the presence of aflatoxin M1 (AFM1) in butter samples marketed in the Black Sea Region of Turkey with two complementary analytical methods to improve accuracy. A total of 40 samples were analysed by enzyme-linked immuno absorbent assay (ELISA) as the screening test and high performance liquid chromatography (HPLC) with fluorescence detection (FLD) after immunoaffinity column (IAC) clean-up as the confirmatory method. Results indicated that butter samples in the Black Sea region were of good quality with respect to AFM1, with no contamination detected. Furthermore, the accurate and sensitive IAC/HPLC-FLD method was confirmed as being appropriate for the detection of AFM1 in butter.
Anahtar Kelime: Konular:
Belge Türü: Makale Makale Türü: Kısa Bildiri Erişim Türü: Erişime Açık
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| APA | PROCEDURES E, Atmaca E, YAZICI F, Güvenç D, Gul O, DERVİŞOĞLU M (2016). Comparative Analysis of Aflatoxin M1 in Marketed Butter by ELISA and HPLC. , 619 - 621. |
| Chicago | PROCEDURES ELISA,Atmaca Enes,YAZICI Fehmi,Güvenç Dilek,Gul Osman,DERVİŞOĞLU Muhammet Comparative Analysis of Aflatoxin M1 in Marketed Butter by ELISA and HPLC. (2016): 619 - 621. |
| MLA | PROCEDURES ELISA,Atmaca Enes,YAZICI Fehmi,Güvenç Dilek,Gul Osman,DERVİŞOĞLU Muhammet Comparative Analysis of Aflatoxin M1 in Marketed Butter by ELISA and HPLC. , 2016, ss.619 - 621. |
| AMA | PROCEDURES E,Atmaca E,YAZICI F,Güvenç D,Gul O,DERVİŞOĞLU M Comparative Analysis of Aflatoxin M1 in Marketed Butter by ELISA and HPLC. . 2016; 619 - 621. |
| Vancouver | PROCEDURES E,Atmaca E,YAZICI F,Güvenç D,Gul O,DERVİŞOĞLU M Comparative Analysis of Aflatoxin M1 in Marketed Butter by ELISA and HPLC. . 2016; 619 - 621. |
| IEEE | PROCEDURES E,Atmaca E,YAZICI F,Güvenç D,Gul O,DERVİŞOĞLU M "Comparative Analysis of Aflatoxin M1 in Marketed Butter by ELISA and HPLC." , ss.619 - 621, 2016. |
| ISNAD | PROCEDURES, ELISA vd. "Comparative Analysis of Aflatoxin M1 in Marketed Butter by ELISA and HPLC". (2016), 619-621. |
| APA | PROCEDURES E, Atmaca E, YAZICI F, Güvenç D, Gul O, DERVİŞOĞLU M (2016). Comparative Analysis of Aflatoxin M1 in Marketed Butter by ELISA and HPLC. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 22(4), 619 - 621. |
| Chicago | PROCEDURES ELISA,Atmaca Enes,YAZICI Fehmi,Güvenç Dilek,Gul Osman,DERVİŞOĞLU Muhammet Comparative Analysis of Aflatoxin M1 in Marketed Butter by ELISA and HPLC. Kafkas Üniversitesi Veteriner Fakültesi Dergisi 22, no.4 (2016): 619 - 621. |
| MLA | PROCEDURES ELISA,Atmaca Enes,YAZICI Fehmi,Güvenç Dilek,Gul Osman,DERVİŞOĞLU Muhammet Comparative Analysis of Aflatoxin M1 in Marketed Butter by ELISA and HPLC. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, vol.22, no.4, 2016, ss.619 - 621. |
| AMA | PROCEDURES E,Atmaca E,YAZICI F,Güvenç D,Gul O,DERVİŞOĞLU M Comparative Analysis of Aflatoxin M1 in Marketed Butter by ELISA and HPLC. Kafkas Üniversitesi Veteriner Fakültesi Dergisi. 2016; 22(4): 619 - 621. |
| Vancouver | PROCEDURES E,Atmaca E,YAZICI F,Güvenç D,Gul O,DERVİŞOĞLU M Comparative Analysis of Aflatoxin M1 in Marketed Butter by ELISA and HPLC. Kafkas Üniversitesi Veteriner Fakültesi Dergisi. 2016; 22(4): 619 - 621. |
| IEEE | PROCEDURES E,Atmaca E,YAZICI F,Güvenç D,Gul O,DERVİŞOĞLU M "Comparative Analysis of Aflatoxin M1 in Marketed Butter by ELISA and HPLC." Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 22, ss.619 - 621, 2016. |
| ISNAD | PROCEDURES, ELISA vd. "Comparative Analysis of Aflatoxin M1 in Marketed Butter by ELISA and HPLC". Kafkas Üniversitesi Veteriner Fakültesi Dergisi 22/4 (2016), 619-621. |
