Effect of lisinopril on oxidative stress in brain tissues of rats with L-Name induced hypertension

KOCAK, Ahmet; Kırbaş, Aynur; SÜTÇÜ, Recep; KIRBAŞ, Serkan; UZAR, Ertuğrul; KUTLUHAN, SÜLEYMAN

Effect of lisinopril on oxidative stress in brain tissues of rats with L-Name induced hypertension

Serkan KIRBAŞ, (Recep Tayyip Erdogan University)

SÜLEYMAN KUTLUHAN, (Recep Tayyip Erdogan University)

Aynur KIRBAŞ, (Recep Tayyip Erdogan University)

Recep SÜTÇÜ, (Katip Celebi University)

Ahmet KOÇAK, (Dumlupınar University)

Ertuğrul UZAR (Dicle University, Faculty of Medicine, Deparment of Neurology, Diyarbakir, Turkey)

Türk Biyokimya Dergisi

1 0

Yıl: 2013 Cilt: 38 Sayı: 2 Sayfa Aralığı: 163 - 168 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022

Effect of lisinopril on oxidative stress in brain tissues of rats with L-Name induced hypertension

Öz:

Amaç: Arteriyel hipertansiyon sıklıkla oksidatif stresle bağlantılı patolojilerle ilişkilidir. Anjiotensin Dönüştürücü Enzim (ADE) inhibitörleri hipertansiyon ve koroner kalp hastalıkları tedavisinde etkili ve güvenilir bir şekilde kullanılmaktadır. Ancak hipertansiyonla ilişkili serebrovasküler ve nörodejeneratif hastalıklardaki ADE inhibitörleri kullanımının önemi henüz netlik kazanmamıştır. Bu çalışmada, bir ADE inhibitörü olan lisinoprilin L-NAME (N? -nitro-L-Arjinin Metil Ester hidroklorid) ile hipertansiyon oluşturulan sıçanların beyin dokularındaki oksidatif stres ve antioksidan enzim aktiviteleri üzerine olan etkisini araştırmayı amaçladık. Yöntem: 32 adet Sprague-Dawley cinsi sıçan 4 gruba bölündü: Kontrol, L-Name, L-Name ve lisinopril, sadece lisinopril. Hipertansiyon, sıçanların içme sularına 75mg/kg L-Name katılıp, ağız yoluyla 6 hafta verilerek oluşturuldu. Sıçanlar 6 hafta süreyle 10mg/kg dozunda lisinopril ile tedavi edildi. Tail cuff metoduyla birinci, üçüncü ve altıncı haftalarda sistolik kan basınçları ölçüldü. Malondialdehid (MDA), süperoksit dismutaz (SOD), katalaz (CAT) ve glutatyon peroksidaz (GSH-Px) enzim aktiviteleri beyin dokusundan ölçüldü. Nitrik oksit (NO) seviyeleri ise plazmadan ölçüldü. Bulgular: Bizim sonuçlar L-NAME'nin hayvanların sistolik kan basıncında artışa yol açtığını göstermiştir. Lisinoprilin antihipertansif etkisi gözlendi. L-Name verilen grupta malondialdehid seviyeleri önemli oranda arttı ve antioksidan enzim aktiviteleri azaldı (p<0.05). Bunun yanında lisinopril verilen grup ile diğer gruplar arasında antioksidan enzim aktivitelerine göre istatistiksel olarak anlamlı farklılık görülmedi (p>0.05). Sonuçlar: Çalışmamızda, hipertansiyon beyin dokusunda oksidatif hasara yol açmıştır. Lisinopril hipertansiyon nedenli oksidatif hasarı önlemesine rağmen, doğrudan antioksidan etkilere sahip değildi. Lisinoprilin beyin dokusundaki etkisinin kesinleşmesi için ileri çalışmalara ihtiyaç vardır

Anahtar Kelime:

Konular: Biyokimya ve Moleküler Biyoloji

L-Name ile hipertansiyon oluşturulan sıçanların beyin dokularındaki oksidatif stres üzerine lisinoprilin etkisi

Öz:

Objective: Arterial hypertension is often associated with pathologies related with oxidative stress. Angiotensin converting enzyme (ACE) inhibitors have been used as a safe and effective treatment of hypertension and coronary heart disease. However, the significance of ACE inhibitor usage in hypertension-induced cerebrovascular and neurodegenerative diseases is still unknown. In this study, we aimed to investigate the effects of lisinopril, an ACE inhibitor, on oxidative stress and antioxidant enzyme activities in brain tissues of rats with L-NAME (Nω-Nitro-L-Arginine Methyl Ester hydrochloride) induced hypertension. Methods: Thirty-two Sprague-Dawley rats were divided into four groups: Control, L-NAME, L-NAME plus lisinopril, and only lisinopril. Hypertension was induced by oral administration of the L-NAME (75 mg/kg/day) in drinking water for 6 weeks. Rats were treated with Lisinopril (10 mg/kg/day) for six weeks. Systolic blood pressures were measured at the first, third and sixth weeks by using tail cuff method. Malondialdehyde (MDA), Superoxide dismutase (SOD), Catalase (CAT) and Glutathione peroxidase (GSH-Px) activity were measured from the brain tissue. Nitric oxide (NO) levels were measured from plasma. Results: Our results showed that L-NAME leads to an increase in systolic blood pressure of animals. The antihypertensive effect of lisinopril was observed. MDA level was significantly increased, and antioxidant enzymes activities were decreased in L-NAME given group (p<0.05). However, there was no statistically significant differences between the lisinopril given and other groups according to antioxidant enzymes activities (p>0.05). Conclusion: In our study, hypertension led to oxidative damage in brain tissues. Although lisinopril prevents the hypertension induced oxidative damage, direct antioxidant effect was not observed. Further studies are needed in order to gain certainty effect of lisinopril in brain tissue.

Anahtar Kelime:

Konular: Biyokimya ve Moleküler Biyoloji

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	KIRBAŞ S, KUTLUHAN S, Kırbaş A, SÜTÇÜ R, KOCAK A, UZAR E (2013). Effect of lisinopril on oxidative stress in brain tissues of rats with L-Name induced hypertension. , 163 - 168.
Chicago	KIRBAŞ Serkan,KUTLUHAN SÜLEYMAN,Kırbaş Aynur,SÜTÇÜ Recep,KOCAK Ahmet,UZAR Ertuğrul Effect of lisinopril on oxidative stress in brain tissues of rats with L-Name induced hypertension. (2013): 163 - 168.
MLA	KIRBAŞ Serkan,KUTLUHAN SÜLEYMAN,Kırbaş Aynur,SÜTÇÜ Recep,KOCAK Ahmet,UZAR Ertuğrul Effect of lisinopril on oxidative stress in brain tissues of rats with L-Name induced hypertension. , 2013, ss.163 - 168.
AMA	KIRBAŞ S,KUTLUHAN S,Kırbaş A,SÜTÇÜ R,KOCAK A,UZAR E Effect of lisinopril on oxidative stress in brain tissues of rats with L-Name induced hypertension. . 2013; 163 - 168.
Vancouver	KIRBAŞ S,KUTLUHAN S,Kırbaş A,SÜTÇÜ R,KOCAK A,UZAR E Effect of lisinopril on oxidative stress in brain tissues of rats with L-Name induced hypertension. . 2013; 163 - 168.
IEEE	KIRBAŞ S,KUTLUHAN S,Kırbaş A,SÜTÇÜ R,KOCAK A,UZAR E "Effect of lisinopril on oxidative stress in brain tissues of rats with L-Name induced hypertension." , ss.163 - 168, 2013.
ISNAD	KIRBAŞ, Serkan vd. "Effect of lisinopril on oxidative stress in brain tissues of rats with L-Name induced hypertension". (2013), 163-168.

APA	KIRBAŞ S, KUTLUHAN S, Kırbaş A, SÜTÇÜ R, KOCAK A, UZAR E (2013). Effect of lisinopril on oxidative stress in brain tissues of rats with L-Name induced hypertension. Türk Biyokimya Dergisi, 38(2), 163 - 168.
Chicago	KIRBAŞ Serkan,KUTLUHAN SÜLEYMAN,Kırbaş Aynur,SÜTÇÜ Recep,KOCAK Ahmet,UZAR Ertuğrul Effect of lisinopril on oxidative stress in brain tissues of rats with L-Name induced hypertension. Türk Biyokimya Dergisi 38, no.2 (2013): 163 - 168.
MLA	KIRBAŞ Serkan,KUTLUHAN SÜLEYMAN,Kırbaş Aynur,SÜTÇÜ Recep,KOCAK Ahmet,UZAR Ertuğrul Effect of lisinopril on oxidative stress in brain tissues of rats with L-Name induced hypertension. Türk Biyokimya Dergisi, vol.38, no.2, 2013, ss.163 - 168.
AMA	KIRBAŞ S,KUTLUHAN S,Kırbaş A,SÜTÇÜ R,KOCAK A,UZAR E Effect of lisinopril on oxidative stress in brain tissues of rats with L-Name induced hypertension. Türk Biyokimya Dergisi. 2013; 38(2): 163 - 168.
Vancouver	KIRBAŞ S,KUTLUHAN S,Kırbaş A,SÜTÇÜ R,KOCAK A,UZAR E Effect of lisinopril on oxidative stress in brain tissues of rats with L-Name induced hypertension. Türk Biyokimya Dergisi. 2013; 38(2): 163 - 168.
IEEE	KIRBAŞ S,KUTLUHAN S,Kırbaş A,SÜTÇÜ R,KOCAK A,UZAR E "Effect of lisinopril on oxidative stress in brain tissues of rats with L-Name induced hypertension." Türk Biyokimya Dergisi, 38, ss.163 - 168, 2013.
ISNAD	KIRBAŞ, Serkan vd. "Effect of lisinopril on oxidative stress in brain tissues of rats with L-Name induced hypertension". Türk Biyokimya Dergisi 38/2 (2013), 163-168.