Effects of melatonin treatment on the spermatogenesis and serum inflammatory cytokine levels in diabetic rats

Aliseydi BOZKURT, (Erzincan Binali Yıldırım University)
Mehmet KARABAKAN, (Mersin Toros State Hospital)
MERVE AYDIN TERZİOĞLU, (KTO Karatay University)
Sinem GUMUSTAS, (Cukurova Askim Tufekci State Hospital)
Didem ONK, (Erzincan Binali Yildirim University)
Cebrail GÜRSUL, (Erzincan Binali Yildirim University)
Hüseyin Serkan EROL (Ataturk University)
Annals of Medical Research
5 1
Yıl: 2019 Cilt: 26 Sayı: 1 Sayfa Aralığı: 12 - 16 Metin Dili: İngilizce DOI: 10.5455/annalsmedres.2018.09.192 İndeks Tarihi: 17-12-2019

Effects of melatonin treatment on the spermatogenesis and serum inflammatory cytokine levels in diabetic rats

Öz:
Aim: Diabetes mellitus (DM) is known to have side effects on sexual and reproductive functions in males. The aim of current study was to investigate the effect of melatonin treatment on spermatogenesis and serum inflammatory cytokine levels in diabetic rats.Material and Method: Eighteen adult Wistar albino male rats were divided randomly into three groups each containing 6 rats; control group (group 1), diabetic group (group 2), diabetic treated with melatonin group (group 3). Experimental diabetes was obtained by administrating 50 mg/kg streptozotocin. Melatonin treatment was administered intraperitoneally for 7 days at a dose of 20 mg, daily. Serum levels of interleukin-1 beta (IL-1β), interleukin 6 (IL-6) and tumor necrosis factor-alpha (TNF-α) were assayed. Spermatogenesis was evaluated according to Johnsen score at immunopathological examination.Results: The Johnsen scores in control, diabetic and treatment groups were 9.78, 8.91, and 9.61, respectively. Spermatogenesis was negatively affected in diabetic rats and significantly improved with melatonin therapy (p<0.05). The serum levels of TNF-α in diabetic rats were observed to be increased compared to the control group (p<0.05). The levels of serum IL-1β and TNF- α were significantly decreased with melatonin treatment. IL-6 levels were not different among the three groups (p=0.248).Conclusions: In the present study, intraperitoneal melatonin treatment was determined to have a positive effect of on spermatogenesis and caused a decrease in serum inflammatory cytokine levels in diabetic rats.
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  • 1. Belotto MF, Magdalon J, Rodrigues HG, et al. Moderate exercise improves leucocyte function and decreases inflammation in diabetes. Clin Exp Immunol 2010;162: 237-43.
  • 2. Coskun O, Ocakci A, Bayraktaroglu T, et al. Exercise training prevents and protects streptozotocin-induced oxidative stress and beta-cell damage in rat pancreas. Tohoku J Exp Med 2004;203:145-54.
  • 3. Kim ST, Moley KH. Paternal effect on embryo quality in diabetic mice is related to poor sperm quality and associated with decreased glucose transporter expression. Reproduction 2008;136:313-22.
  • 4. Scarano WR, Messias AG, Oliva SU, et al. Sexual behaviour, sperm quantity and quality after short-therm streptozotocin-induced hyperglycaemia in rats. Int J Androl 2006;29: 482-8.
  • 5. Mangoli E, Talebi AR, Anvari M, et al. Effects of experimentally-induced diabetes on sperm parameters and chromatin quality in mice. Iran J Reprod Med 2013;11:53-60.
  • 6. Meyer K, Deutscher J, Anil M, et al. Serum androgen levels in adolescents with type 1 diabetes: relationship to pubertal stage and metabolic control. J Endocrinol Invest 2000;23:362-8.
  • 7. Guneli E, Tugyan K, Ozturk H, et al. Effect of melatonin on testicular damage in streptozotocin-induced diabetes rats. Eur Surg Res 2008;40: 354-60.
  • 8. Cai L, Chen S, Evans T, et al. Apoptotic germ-cell death and testicular damage in experimental diabetes: prevention by endothelin antagonism. Urol Res 2000;28:342-7.
  • 9. Dandona P, Aljada A, Chaudhuri A, et al. Metabolic syndrome: a comprehensive perpective based on interactions between obesity, diabetes, and inflammation. Circulation 2005;111:1448-54.
  • 10. Khaki A, Nouri M, Fathiazad F, Protective effects of quercetin on spermatogenesis in streptozotocin-induced diabetic rat. J Med Plants Res 2009;8:57-64.
  • 11. Grimaldi P, Di Giacomo D, Geremia R. The endocannabinoid system and spermatogenesis. Front Endocrinol (Lausanne) 2013;4:192.
  • 12. Huleihel M, Lunenfeld E. Regulation of spermatogenesis by paracrine/autocrine testicular factors. Asian J Androl 2004;6:259-68.
  • 13. Lysiak JJ. The role of tumor necrosis factor-alpha and interleukin-1 in the mammalian testis and their involvement in testicular torsion and autoimmune orchitis. Reprod Biol Endocrinol 2004;2:9.
  • 14. Lui WY, Lee WM. Molecular mechanisms by which hormones and cytokines regulate cell junction dynamics in the testis. J Mol Endocrinol 2009;43:43-51.
  • 15. Budhwar S, Singh V, Verma P, et al. Fertilization failure and gamete health: Is there a link? Front Biosci (Schol Ed) 2017;9:395-419.
  • 16. Huleihel M, Abuelhija M, Lunenfeld E. In vitro culture of testicular germ cells: regulatory factors and limitations. Growth Factors 2007;25:236-52.
  • 17. Arango Dugue G, Descoteaux A. Macrophage cytokines: involvement in immunity and infectious diseases. Front Immunol 2014;5:491.
  • 18. Okuma Y, Saito K, O’Connor AE, et al. Reciprocal regulation of activin A and inhibin B by interleukim-1 (IL-1) and follicle-stimulating hormone (FSH) in rat Sertoli cells in vitro. J Endocrinol 2005;185:99-110.
  • 19. Riccioli A, Starace D, D’Alessio A, et al. TNF-alpha and IFN-gamma regulate expression and function of the Fas system in the seminiferous epithelium. J Immunol 2000;165:743-9.
  • 20. Hales DB. Testicular macrophage modulation of Leydig cell steroidogenesis. J Reprod Immunol 2002;57:3-18.
  • 21. Azenabor A, Ekun AO, Akinloye O. Impact of inflammation on male reproductive tract. J Reprod Infertil 2015;16:123-9.
  • 22. O’Bryan MK, Hedger MP. Inflammatory networks in the control of spermatogenesis: chronic inflammation in an immunologically privileged tissue? Adv Exp Med Biol 2008;636:92-114.
  • 23. Reiter RJ, Tan DX, Osuna C, et al. Actions of melatonin in the reduction of oxidative stress. A review. J Biomed Sci 2000;7:444-58.
  • 24. Reiter RJ, Tan DX, Terron MP, et al. Melatonin and its metabolites: new findings regarding their production and their radical scavenging actions. Acta Biochim Pol 2007;54:1-9.
  • 25. Tan DX, Chen LD, Poeggeler B, et al. Melatonin: a potent, endogenous hydroxyl radical scavenger. Endocr J 1993;1:57-60.
  • 26. Hemadi M, Zargar M, Sobhani A, et al. Assessment of morphological and functional changes in neonate vitrified testis grafts after host treatment with melatonin. Folia Morphol (Warsz) 2011;70:95-102.
  • 27. Ghasemi FM, Faghani M, Khajehjahromi S, et al. Effect of melatonin on proliferative activity and apoptosis in spermatogenic cells in mouse under chemotheraphy. J Reprod Contracept 2010;21:79-94.
  • 28. Mirhoseini M, Saki G, Hemadi M, et al. Melatonin and testicular damage in busulfan treated mice. Iran Red Crescent Med J 2014;16:e14463.
  • 29. Nasiraei-Moghadam SN, Parivar K, Mohammad Reza VM, et al. Protective effect of melatonin against inequality-induced damages on testicular tissue and sperm para meters. Int J Fertil Steril 2014;7:313-22.
  • 30. Al-Awar A, Kupai K, Veszelka M et al. Experimental diabetes mellitus in different animal models. J Diabetes Res 2016;2016:9051426.
  • 31. Erdemir F, Atilgan D, Markoc F, et al. The effect of diet induced obesity on testicular tissue and serum oxidative stress parameters. Actas Urol Esp 2012;36:153-9.
  • 32. Emami NH, Lafout FM, Mohammadghasemi F. Administration of melatonin protects against acetylsalicylic acid-induced impairment of male reproductive function in mice. Iran J Basic Med Sci 2018;21:124-9.
  • 33. Sexton WJ, Jarow JP. Effect of diabetes mellitus upon male reproductive function. Urology 1997;49:508-13.
  • 34. Baker HW. Reproductive effects of nontesticular illness. Endocrinol Metab Clin North Am 1998; 27:831-50.
  • 35. Aprioku JS. Pharmacology of free radicals and the impact of reactive oxygen species on the testis. J Reprod Infertil 2013;14:158-72.
  • 36. Hardeland R, Tan DX, Reiter RJ. Kynuramines, metabolites of melatonin and other indoles: the resurrection of an almost forgotten class of biogenic amines. J Pineal Res 2009;47:109-26.
  • 37. Mohammad Ghasemi F, Faghani M, Falah Karkan M. The protective effect of melatonin on sperm parameters, epididymis and seminal vesicle morphology in adult mouse treated with busulfan. J Iran Anatomi Sci 2010;8:25-36.
  • 38. Cheng CY, Wong EW, Yan HH, et al. Regulation of spermatogenesis in the microenvironment of the seminiferous epithelium: new insights and advances. Moll Cell Endocrinol 2010;315:49-56.
APA Bozkurt A, karabakan M, AYDIN TERZİOĞLU M, GUMUSTAS S, ONK D, GÜRSUL C, Erol H (2019). Effects of melatonin treatment on the spermatogenesis and serum inflammatory cytokine levels in diabetic rats. , 12 - 16. 10.5455/annalsmedres.2018.09.192
Chicago Bozkurt Aliseydi,karabakan Mehmet,AYDIN TERZİOĞLU MERVE,GUMUSTAS Sinem,ONK Didem,GÜRSUL Cebrail,Erol Huseyin Serkan Effects of melatonin treatment on the spermatogenesis and serum inflammatory cytokine levels in diabetic rats. (2019): 12 - 16. 10.5455/annalsmedres.2018.09.192
MLA Bozkurt Aliseydi,karabakan Mehmet,AYDIN TERZİOĞLU MERVE,GUMUSTAS Sinem,ONK Didem,GÜRSUL Cebrail,Erol Huseyin Serkan Effects of melatonin treatment on the spermatogenesis and serum inflammatory cytokine levels in diabetic rats. , 2019, ss.12 - 16. 10.5455/annalsmedres.2018.09.192
AMA Bozkurt A,karabakan M,AYDIN TERZİOĞLU M,GUMUSTAS S,ONK D,GÜRSUL C,Erol H Effects of melatonin treatment on the spermatogenesis and serum inflammatory cytokine levels in diabetic rats. . 2019; 12 - 16. 10.5455/annalsmedres.2018.09.192
Vancouver Bozkurt A,karabakan M,AYDIN TERZİOĞLU M,GUMUSTAS S,ONK D,GÜRSUL C,Erol H Effects of melatonin treatment on the spermatogenesis and serum inflammatory cytokine levels in diabetic rats. . 2019; 12 - 16. 10.5455/annalsmedres.2018.09.192
IEEE Bozkurt A,karabakan M,AYDIN TERZİOĞLU M,GUMUSTAS S,ONK D,GÜRSUL C,Erol H "Effects of melatonin treatment on the spermatogenesis and serum inflammatory cytokine levels in diabetic rats." , ss.12 - 16, 2019. 10.5455/annalsmedres.2018.09.192
ISNAD Bozkurt, Aliseydi vd. "Effects of melatonin treatment on the spermatogenesis and serum inflammatory cytokine levels in diabetic rats". (2019), 12-16. https://doi.org/10.5455/annalsmedres.2018.09.192
APA Bozkurt A, karabakan M, AYDIN TERZİOĞLU M, GUMUSTAS S, ONK D, GÜRSUL C, Erol H (2019). Effects of melatonin treatment on the spermatogenesis and serum inflammatory cytokine levels in diabetic rats. Annals of Medical Research, 26(1), 12 - 16. 10.5455/annalsmedres.2018.09.192
Chicago Bozkurt Aliseydi,karabakan Mehmet,AYDIN TERZİOĞLU MERVE,GUMUSTAS Sinem,ONK Didem,GÜRSUL Cebrail,Erol Huseyin Serkan Effects of melatonin treatment on the spermatogenesis and serum inflammatory cytokine levels in diabetic rats. Annals of Medical Research 26, no.1 (2019): 12 - 16. 10.5455/annalsmedres.2018.09.192
MLA Bozkurt Aliseydi,karabakan Mehmet,AYDIN TERZİOĞLU MERVE,GUMUSTAS Sinem,ONK Didem,GÜRSUL Cebrail,Erol Huseyin Serkan Effects of melatonin treatment on the spermatogenesis and serum inflammatory cytokine levels in diabetic rats. Annals of Medical Research, vol.26, no.1, 2019, ss.12 - 16. 10.5455/annalsmedres.2018.09.192
AMA Bozkurt A,karabakan M,AYDIN TERZİOĞLU M,GUMUSTAS S,ONK D,GÜRSUL C,Erol H Effects of melatonin treatment on the spermatogenesis and serum inflammatory cytokine levels in diabetic rats. Annals of Medical Research. 2019; 26(1): 12 - 16. 10.5455/annalsmedres.2018.09.192
Vancouver Bozkurt A,karabakan M,AYDIN TERZİOĞLU M,GUMUSTAS S,ONK D,GÜRSUL C,Erol H Effects of melatonin treatment on the spermatogenesis and serum inflammatory cytokine levels in diabetic rats. Annals of Medical Research. 2019; 26(1): 12 - 16. 10.5455/annalsmedres.2018.09.192
IEEE Bozkurt A,karabakan M,AYDIN TERZİOĞLU M,GUMUSTAS S,ONK D,GÜRSUL C,Erol H "Effects of melatonin treatment on the spermatogenesis and serum inflammatory cytokine levels in diabetic rats." Annals of Medical Research, 26, ss.12 - 16, 2019. 10.5455/annalsmedres.2018.09.192
ISNAD Bozkurt, Aliseydi vd. "Effects of melatonin treatment on the spermatogenesis and serum inflammatory cytokine levels in diabetic rats". Annals of Medical Research 26/1 (2019), 12-16. https://doi.org/10.5455/annalsmedres.2018.09.192
Sayfa Oluşturulma Tarihi
23-04-2024 9:32

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