The Effects of Squirting Cucumber (Ecballium elaterium l. 1758) on the Blood Cell Morphology of Common Carp (Cyprinus carpio L. 1758)
Yıl: 2020 Cilt: 16 Sayı: 3 Sayfa Aralığı: 400 - 408 Metin Dili: İngilizce DOI: 10.22392/actaquatr.700201 İndeks Tarihi: 28-10-2020
The Effects of Squirting Cucumber (Ecballium elaterium l. 1758) on the Blood Cell Morphology of Common Carp (Cyprinus carpio L. 1758)
Öz: It is well-known that for centuries, humans have used plants and herbal remedies to treat many diseases, in addition to thetreatment options brought and applied by modern medicine, which relies on scientific studies and actual evidence. One ofthese plants is the squirting cucumber (Ecballium elaterium), or the exploding cucumber. Despite its therapeutic properties,the squirting cucumber may also cause serious biological damage to humans, as demonstrated several studies. This study wasconducted to determine whether the juice obtained from ripe fruits of squirting cucumber has any effect on the cellmorphology of common carp (Cyprinus carpio). Juice application was carried out on 4 groups, composed of 1 control and 3experimental groups. Squirting cucumber juice was added to three aquariums containing different experimental groups, inthree different ppm (100, 200, and 300) levels, for 72 hours. Afterwards, blood smear samples of the fish were prepared, andthe preparations were scanned under a microscope to determine the changes in blood cell morphology. The resultsdemonstrated that squirting cucumber juice caused micronucleus formation during cell division in C. carpio blood cells, andseveral abnormalities in blood cell morphology including Lobed nucleus (LB), Bud nucleus (BL), Notched nucleus (NT) andBinucleated erythrocytes (BN).
Anahtar Kelime: Acı Kavun (Ecballıum Elaterıum L. 1758)’un Sazan (Cyprınus Carpıo L. 1758) Balıkları Kan Hücre Morfolojilerine Etkileri
Öz: Yüzyıllardır birçok hastalığın tedavisinde modern tıp tarafından uygulanan, kanıta ve bilimsel çalışmalara dayalı tedavi seçeneklerinin yanı sıra bitkiler ve bitkisel ilaçların da kullanıldığı bilinmektedir. Tedavi amaçlı kullanılan bitkilerden bir tanesi acı kavun (Ecballium elaterium) bitkisidir. Acı kavun bitkinin tedavi edici özelliğinin yan ısıra, insanlar üzerinde ciddi biyolojik hasarlar da bıraktığı yapılan çalışmalarda görülmektedir. Bu çalışmada, acı kavun bitkisine ait olgun meyvelerden elde edilen özsuyun sazan balıkları (Cyprinus carpio)’nın hücre morfolojilerine etkisinin olup olmadığının belirlenmesi amaçlanmıştır. Uygulama 3 deney ve 1 kontrol grubu olmak üzere 4 grup üzerinde yapılmıştır. Üç farklı deney grubuna ait akvaryumlara 3 farklı ppm (100, 200 ve 300) değerinde acı kavun özsuyu 72 saat uygulanmıştır. Balıklardan kan yayma preparatları hazırlanmıştır ve hazırlanan preparatlar mikroskop altında taranarak kan hücrelerinin morfolojilerinde meydana gelen değişiklikler tespit edilmiştir. Sonuç olarak acı kavun özsuyunun C. carpio kan hücrelerinde bölünme esnasında mikronükleus oluşumuna neden olduğu ve kan hücrelerinin morfolojilerinde Loblu nükleus (LB), Tomurcuklu nükleus (BL), Çentikli nükleus (NT) ve Binükleat (BN) gibi anormalliklere neden olduğu tespit edilmiştir.
Anahtar Kelime: Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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| APA | SEYFİ E, Gaffaroglu M (2020). The Effects of Squirting Cucumber (Ecballium elaterium l. 1758) on the Blood Cell Morphology of Common Carp (Cyprinus carpio L. 1758). , 400 - 408. 10.22392/actaquatr.700201 |
| Chicago | SEYFİ EMİN,Gaffaroglu Muhammet The Effects of Squirting Cucumber (Ecballium elaterium l. 1758) on the Blood Cell Morphology of Common Carp (Cyprinus carpio L. 1758). (2020): 400 - 408. 10.22392/actaquatr.700201 |
| MLA | SEYFİ EMİN,Gaffaroglu Muhammet The Effects of Squirting Cucumber (Ecballium elaterium l. 1758) on the Blood Cell Morphology of Common Carp (Cyprinus carpio L. 1758). , 2020, ss.400 - 408. 10.22392/actaquatr.700201 |
| AMA | SEYFİ E,Gaffaroglu M The Effects of Squirting Cucumber (Ecballium elaterium l. 1758) on the Blood Cell Morphology of Common Carp (Cyprinus carpio L. 1758). . 2020; 400 - 408. 10.22392/actaquatr.700201 |
| Vancouver | SEYFİ E,Gaffaroglu M The Effects of Squirting Cucumber (Ecballium elaterium l. 1758) on the Blood Cell Morphology of Common Carp (Cyprinus carpio L. 1758). . 2020; 400 - 408. 10.22392/actaquatr.700201 |
| IEEE | SEYFİ E,Gaffaroglu M "The Effects of Squirting Cucumber (Ecballium elaterium l. 1758) on the Blood Cell Morphology of Common Carp (Cyprinus carpio L. 1758)." , ss.400 - 408, 2020. 10.22392/actaquatr.700201 |
| ISNAD | SEYFİ, EMİN - Gaffaroglu, Muhammet. "The Effects of Squirting Cucumber (Ecballium elaterium l. 1758) on the Blood Cell Morphology of Common Carp (Cyprinus carpio L. 1758)". (2020), 400-408. https://doi.org/10.22392/actaquatr.700201 |
| APA | SEYFİ E, Gaffaroglu M (2020). The Effects of Squirting Cucumber (Ecballium elaterium l. 1758) on the Blood Cell Morphology of Common Carp (Cyprinus carpio L. 1758). Acta Aquatica Turcica, 16(3), 400 - 408. 10.22392/actaquatr.700201 |
| Chicago | SEYFİ EMİN,Gaffaroglu Muhammet The Effects of Squirting Cucumber (Ecballium elaterium l. 1758) on the Blood Cell Morphology of Common Carp (Cyprinus carpio L. 1758). Acta Aquatica Turcica 16, no.3 (2020): 400 - 408. 10.22392/actaquatr.700201 |
| MLA | SEYFİ EMİN,Gaffaroglu Muhammet The Effects of Squirting Cucumber (Ecballium elaterium l. 1758) on the Blood Cell Morphology of Common Carp (Cyprinus carpio L. 1758). Acta Aquatica Turcica, vol.16, no.3, 2020, ss.400 - 408. 10.22392/actaquatr.700201 |
| AMA | SEYFİ E,Gaffaroglu M The Effects of Squirting Cucumber (Ecballium elaterium l. 1758) on the Blood Cell Morphology of Common Carp (Cyprinus carpio L. 1758). Acta Aquatica Turcica. 2020; 16(3): 400 - 408. 10.22392/actaquatr.700201 |
| Vancouver | SEYFİ E,Gaffaroglu M The Effects of Squirting Cucumber (Ecballium elaterium l. 1758) on the Blood Cell Morphology of Common Carp (Cyprinus carpio L. 1758). Acta Aquatica Turcica. 2020; 16(3): 400 - 408. 10.22392/actaquatr.700201 |
| IEEE | SEYFİ E,Gaffaroglu M "The Effects of Squirting Cucumber (Ecballium elaterium l. 1758) on the Blood Cell Morphology of Common Carp (Cyprinus carpio L. 1758)." Acta Aquatica Turcica, 16, ss.400 - 408, 2020. 10.22392/actaquatr.700201 |
| ISNAD | SEYFİ, EMİN - Gaffaroglu, Muhammet. "The Effects of Squirting Cucumber (Ecballium elaterium l. 1758) on the Blood Cell Morphology of Common Carp (Cyprinus carpio L. 1758)". Acta Aquatica Turcica 16/3 (2020), 400-408. https://doi.org/10.22392/actaquatr.700201 |
