THE EFFECT OF INTER-PLANTAL COMPETITION ON IN VITRO SEED GERMINATION AND SEEDLING GROWTH IN FLAX (LINUM USITATISSIMUM L.)
Yıl: 2019 Cilt: 8 Sayı: 1 Sayfa Aralığı: 61 - 68 Metin Dili: İngilizce DOI: 10.18036/aubtdc.427128 İndeks Tarihi: 11-01-2021
THE EFFECT OF INTER-PLANTAL COMPETITION ON IN VITRO SEED GERMINATION AND SEEDLING GROWTH IN FLAX (LINUM USITATISSIMUM L.)
Öz: This study was carried out to investigate the relationship between in vitro competition and stress, and their effects on seedlinggrowth of three flax (Linum usitatissimum) cultivars ('Madaras', 'Clarck', and '1886 Sel.'). Competition among plants wasachieved by changing the distances they sown. Four different distances were used as '0.5x0.5', '1.0x1.0', '1.5x1.5' and '2.0x2.0'cm. The results showed that encouraging seedlings for competition by decreasing the distances among seeds sown per Magentavessel elevated chlorophyll contents (chl. a, b and total chl.), seedling height and root length significantly up to a thresholdfrom where stress initiated and significant decreases in all parameters were observed. In term of chlorophyll contents (chl. a, band total chl.), seedling height and root length, the highest results (1540.1, 581.9, and 1253.7 µg/g tissue, 4.54 and 7.77 cm,respectively) were obtained from '1.5x1.5' cm distance in cv. 'Madaras'. For cv. 'Clarck', the highest values in chlorophyllcontents (chl. a, b and total chl.), seedling height and root length were recorded as 1860.6, 652.6, and 1429.0 µg/g tissue, 4.58and 6.79 cm, respectively in '1.5x1.5' cm distance application. The highest results with respect to chlorophyll contents (chl. a,b and total chl.), seedling height and root length were aigain recorded at '1.5x1.5' cm distance application in cv. '1886 Sel.' as1782.6, 552.0, and 1138.7 µg/g tissue, 4.62 and 6.45 cm, respectively. As a consequence, the application of '1.5x1.5' cmdistance seems to best for all cultivars. This study showed that competition among in vitro-grown seedlings which can be thesource of explants may be used to increase the success of tissue culture studies.
Anahtar Kelime: BİTKİLER ARASI REKABETİN KETENDE (LINUM USITATISSIMUM L.) IN VITRO ÇİMLENME VE FİDE GELİŞİMİ ÜZERİNE ETKİSİ
Öz: Bu çalışma, in vitro rekabet ve stress arasındaki ilişkileri ve bunların üç keten (Linum usitatissimum) çeşidinde fide gelişimi üzerine etkilerini araştırmak için yapılmıştır. Bitkiler arasındaki rekabet, tohumların ekildiği mesafeler değiştirilerek sağlanmıştır. '0.5x0.5', '1.0x1.0', '1.5x1.5' ve '2.0x2.0' cm olarak dört farklı mesafe kullanılmıştır. Sonuçlar, fidelerin Magenta kaplarına ekilen tohumlar arasındaki mesafenin daraltılması ile elde edilen rekabete özendirilmesinin klorofil kapsamları (klorofil a, klorofil b ve toplam klorofil), fide boyu ve kök uzunluğunu stresin başladığı ve bütün parametrelerde önemli düşüşlerin gözlendiği belli bir noktaya kadar önemli derecede artırdığını göstermiştir. 'Madaras' çeşidinde klorofil içerikleri (klrofil a, klorfil b ve toplam klorfil), fide boyu ve kök uzunluğu bakımından en yüksek sonuçlar (1540.1, 581.9, ve 1253.7 µg/g tissue, 4.54 and 7.77 cm) '1.5x1.5' cm mesafesinden elde edilmiştir. 'Clarck' çeşidi için, klorofil (klorofil a, klorofil b ve toplam klorofil) içerikleri, fide boyu ve kök uzunluğunda en yüksek değerler sırasıyla 1860.6, 652.6, and 1429.0 µg/g tissue, 4.58 ve 6.79 cm olarak 1.5x1.5 cm mesafe uygulamasında kaydedilmiştir. '1886 Sel.' çeşidinde klorofil (klorofil a, klorofil b ve toplam klorofil) içerikleri, fide boyu ve kök uzunluğu bakımından en yüksek değerler sırasıyla 1860.6, 652.6, and 1429.0 µg/g tissue, 4.58 ve 6.79 cm olarak yine '1.5x1.5' cm mesafe uygulamasında kayıt edilmiştir. Sonuç olarak, '1.5x1.5' cm mesafe uygulaması tüm çeşitler için en iyisi olarak görünmektedir. Bu çalışma, eksplant kaynağı olarak kullanılacak in vitro yetiştirilen fideler arasındaki rekabetin doku kültürü çalışmalarının başarısını artırmak için kullanılabileceğini göstermiştir.
Anahtar Kelime: Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
- [1] Wilson JB. Shoot competition and root competition. J Appl Ecol 1988; 25: 279-296.
- [2] McPhee CS, Aarssen LW. The seperation of above- and below-ground competion in plants. A review and critique of methodology. Plant Ecol 2001; 152: 119-136.
- [3] Yıldız M, Özcan, S, Er C. The effect of different explant sources on adventitious shoot regeneration in flax (Linum usitatissimum L.). Turk J. Biol 2002; 26: 37-40.
- [4] Jolliffe PA, Gaye MM. Dynamics of growth and yield component responses of bell peppers (Capsicum annuum L.) to row covers and population density. Scientia Hortic 1995; 62: 153-164.
- [5] Morgade L, Willey R. Effect of plant population and nitrogen fertilizer on yield and efficiency of maize/bean intercropping. Pesq Agropec Bras Brasilia 2003; 38: 1257-1264.
- [6] Daşgan HY, Abak K. Effects of plant density and number of shoots on yield and fruit characteristics of peppers grown in glasshouses. Turk J Agric For 2003; 27: 29-35.
- [7] Abubaker S. Effect of plant density on flowering date, yield and quality attribute of bush beans (Phaseolus vulgaris L.) under center pivot irrigation system. Am J Agr Biol Sci 2008; 3: 666-668.
- [8] Asghari MT, Daneshian J, Farahani AA. Effects of drought stress and planting density on quantity and morphological characteristics of chicory (Cichorium intybus L.). Asian J Agr Sci 2009; 1: 12-14.
- [9] Tawfik RS, Badr A, Sammour R, Ibrahim U, Matter M, Sakr M. Improvement of Flax Drought Tolerance Using Gene Transfer. Plant Tiss Cult Biotech 2016; 26(2): 197‐207.
- [10] Basiran N, Armitage P, Scott RJ, Draper J. Genetic transformation of flax (Linum usitatissimum) by Agrobacterium tumefaciens: regeneration of transformed shoots via a callus phase. Plant Cell Rep 1987; 6: 396-399.
- [11] Dong JZ, Mc Hughen A. Patterns of transformation intensity on flax hypocotyls inoculated with Agrobacterium tumefaciens. Plant Cell Rep 1991; 10: 555-560.
- [12] Millam S, Davidson D, Powell W. The use of flax (Linum usitatissimum) as a model system for studies on organogenesis in vitro: the effect of different carbohydrates. Plant Cell Tiss Org 1992; 28: 163-166.
- [13] Jordan MC, Mc Hughen A. Transformed calllus does not necessarily regenerate transformed shoots. Plant Cell Rep 1988; 7: 285-287.
- [14] Dong JZ, Mc Hughen A. An improved procedure for production of transgenic flax plants using Agrobacterium tumefaciens. Plant Sci 1993; 88: 61-71.
- [15] Yıldız M, Er C. The effect of sodium hypochlorite solutions on in vitro seedling growth and shoot regeneration of flax (Linum usitatissimum). Naturwissenschaften 2002; 89: 259-261.
- [16] Yıldız M, Özcan S, Er C. The effect of different explant sources on adventitious shoot regeneration in flax (Linum usitatissimum L.). Turk J Biol 2002; 26: 37-40.
- [17] Murashige T, Skoog F. A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol Plant 1962; 15: 473-479.
- [18] Curtis OF, Shetty K. Growth medium effects on vitrification, total phenolics, chlorophyll, and water content of in vitro propagated oregano clones. Acta Horticulture 1996; 426: 498-503.
- [19] Yildiz M, Sağlik Ç, Telci C, Erkiliç EG. The effect of in vitro competition on shoot regeneration from hypocotyl explants of Linum usitatissimum. Turk J Bot 2011; 35:211-218.
- [20] Aycan M, Kayan M, Yildiz M. The effect of in vitro spacing competition on shoot regeneration from cotyledon node explants of Lathyrus chrysanthus Boiss. Aust J Crop Sci 2014; 8(7):1019-1023.
- [21] Yildiz M. Evaluation of the effect of in vitro stress and competition on tissue culture response of flax. Biol Plantarum 2011; 55(3): 541-544.
- [22] Posada-Perez L, Montesinos YP, Guerra DG, Daniels D. Complete germination of papaya (Carica papaya L. cv. `Maradol Roja´) somatic embryos using temporary immersion system type RITA® and phloroglucinol in semi-solid culture medium. In Vitro Cell Dev Biol 2011; 53: 505- 513.
- [23] Romero C, Cuba-Diaz M, Silva R. In vitro culture of Chloraea gavilu Lindl., an endemic terrestrial orchid from Chile. Plant Biosyst 2018; 152: 612-620.
| APA | BEYAZ R, YILDIZ M (2019). THE EFFECT OF INTER-PLANTAL COMPETITION ON IN VITRO SEED GERMINATION AND SEEDLING GROWTH IN FLAX (LINUM USITATISSIMUM L.). , 61 - 68. 10.18036/aubtdc.427128 |
| Chicago | BEYAZ Ramazan,YILDIZ Muhammet Mustafa THE EFFECT OF INTER-PLANTAL COMPETITION ON IN VITRO SEED GERMINATION AND SEEDLING GROWTH IN FLAX (LINUM USITATISSIMUM L.). (2019): 61 - 68. 10.18036/aubtdc.427128 |
| MLA | BEYAZ Ramazan,YILDIZ Muhammet Mustafa THE EFFECT OF INTER-PLANTAL COMPETITION ON IN VITRO SEED GERMINATION AND SEEDLING GROWTH IN FLAX (LINUM USITATISSIMUM L.). , 2019, ss.61 - 68. 10.18036/aubtdc.427128 |
| AMA | BEYAZ R,YILDIZ M THE EFFECT OF INTER-PLANTAL COMPETITION ON IN VITRO SEED GERMINATION AND SEEDLING GROWTH IN FLAX (LINUM USITATISSIMUM L.). . 2019; 61 - 68. 10.18036/aubtdc.427128 |
| Vancouver | BEYAZ R,YILDIZ M THE EFFECT OF INTER-PLANTAL COMPETITION ON IN VITRO SEED GERMINATION AND SEEDLING GROWTH IN FLAX (LINUM USITATISSIMUM L.). . 2019; 61 - 68. 10.18036/aubtdc.427128 |
| IEEE | BEYAZ R,YILDIZ M "THE EFFECT OF INTER-PLANTAL COMPETITION ON IN VITRO SEED GERMINATION AND SEEDLING GROWTH IN FLAX (LINUM USITATISSIMUM L.)." , ss.61 - 68, 2019. 10.18036/aubtdc.427128 |
| ISNAD | BEYAZ, Ramazan - YILDIZ, Muhammet Mustafa. "THE EFFECT OF INTER-PLANTAL COMPETITION ON IN VITRO SEED GERMINATION AND SEEDLING GROWTH IN FLAX (LINUM USITATISSIMUM L.)". (2019), 61-68. https://doi.org/10.18036/aubtdc.427128 |
| APA | BEYAZ R, YILDIZ M (2019). THE EFFECT OF INTER-PLANTAL COMPETITION ON IN VITRO SEED GERMINATION AND SEEDLING GROWTH IN FLAX (LINUM USITATISSIMUM L.). Eskişehir Teknik Üniversitesi Bilim ve Teknoloji Dergisi C- yaşam Bilimleri ve Biyoteknoloji (Online), 8(1), 61 - 68. 10.18036/aubtdc.427128 |
| Chicago | BEYAZ Ramazan,YILDIZ Muhammet Mustafa THE EFFECT OF INTER-PLANTAL COMPETITION ON IN VITRO SEED GERMINATION AND SEEDLING GROWTH IN FLAX (LINUM USITATISSIMUM L.). Eskişehir Teknik Üniversitesi Bilim ve Teknoloji Dergisi C- yaşam Bilimleri ve Biyoteknoloji (Online) 8, no.1 (2019): 61 - 68. 10.18036/aubtdc.427128 |
| MLA | BEYAZ Ramazan,YILDIZ Muhammet Mustafa THE EFFECT OF INTER-PLANTAL COMPETITION ON IN VITRO SEED GERMINATION AND SEEDLING GROWTH IN FLAX (LINUM USITATISSIMUM L.). Eskişehir Teknik Üniversitesi Bilim ve Teknoloji Dergisi C- yaşam Bilimleri ve Biyoteknoloji (Online), vol.8, no.1, 2019, ss.61 - 68. 10.18036/aubtdc.427128 |
| AMA | BEYAZ R,YILDIZ M THE EFFECT OF INTER-PLANTAL COMPETITION ON IN VITRO SEED GERMINATION AND SEEDLING GROWTH IN FLAX (LINUM USITATISSIMUM L.). Eskişehir Teknik Üniversitesi Bilim ve Teknoloji Dergisi C- yaşam Bilimleri ve Biyoteknoloji (Online). 2019; 8(1): 61 - 68. 10.18036/aubtdc.427128 |
| Vancouver | BEYAZ R,YILDIZ M THE EFFECT OF INTER-PLANTAL COMPETITION ON IN VITRO SEED GERMINATION AND SEEDLING GROWTH IN FLAX (LINUM USITATISSIMUM L.). Eskişehir Teknik Üniversitesi Bilim ve Teknoloji Dergisi C- yaşam Bilimleri ve Biyoteknoloji (Online). 2019; 8(1): 61 - 68. 10.18036/aubtdc.427128 |
| IEEE | BEYAZ R,YILDIZ M "THE EFFECT OF INTER-PLANTAL COMPETITION ON IN VITRO SEED GERMINATION AND SEEDLING GROWTH IN FLAX (LINUM USITATISSIMUM L.)." Eskişehir Teknik Üniversitesi Bilim ve Teknoloji Dergisi C- yaşam Bilimleri ve Biyoteknoloji (Online), 8, ss.61 - 68, 2019. 10.18036/aubtdc.427128 |
| ISNAD | BEYAZ, Ramazan - YILDIZ, Muhammet Mustafa. "THE EFFECT OF INTER-PLANTAL COMPETITION ON IN VITRO SEED GERMINATION AND SEEDLING GROWTH IN FLAX (LINUM USITATISSIMUM L.)". Eskişehir Teknik Üniversitesi Bilim ve Teknoloji Dergisi C- yaşam Bilimleri ve Biyoteknoloji (Online) 8/1 (2019), 61-68. https://doi.org/10.18036/aubtdc.427128 |
