In Vitro Investigation of the Effects of Imidacloprid on AChE, LDH, and GSH Levels in the L-929 Fibroblast Cell Line
Yıl: 2020 Cilt: 17 Sayı: 5 Sayfa Aralığı: 506 - 510 Metin Dili: İngilizce DOI: 10.4274/tjps.galenos.2019.15807 İndeks Tarihi: 10-05-2021
In Vitro Investigation of the Effects of Imidacloprid on AChE, LDH, and GSH Levels in the L-929 Fibroblast Cell Line
Öz: Objectives: There are several types of pesticides to control pests and several new types coming into use that could be less toxic compared to the old ones. Pesticide-induced oxidative stress, which is one of the main mechanisms of toxicity, is the research area focused most on over the last decade. There are several different studies in the literature on whether pesticide exposure induces oxidative stress parameter-mediated toxicity. Pesticide-induced oxidative stress level depends on the biochemical features of mammalian systems. Imidacloprid is a neonicotinoid pesticide in wide use that is considered safe; however, it has been reported in different studies that it may cause changes in oxidative stress parameters.Materials and Methods: We investigated the dose- and time-dependent effects of imidacloprid on acetylcholinesterase (AChE), lactate dehydrogenase (LDH), and glutathione (GSH) levels in the L-929 fibroblast cell line. The effects of 1-500 μg imidacloprid dose range on AChE, GSH, and LDH were investigated. Results: LDH levels were significantly increased dose dependently in the 250 and 500 ng imidacloprid groups compared to the control group. GSH levels nonsignificantly decreased dose dependently and GSH levels were lower in the 500 ng imidacloprid group compared to the control group. There were no significant differences between the groups in AChE levels. Conclusion: These results indicated that high doses of imidacloprid may induce oxidative stress in fibroblast cells.
Anahtar Kelime: L929 Fibroblast Hücre Hattında İmidoklorit Etkisinin İn Vitro Araştırılması
Öz: Amaç: Haşereleri kontrol altına almak için çeşitli pestisit türleri ve eskilere kıyasla daha az toksik olan yeni tür pestisitler kullanıma giriyor. Toksisitenin ana mekanizmalarından biri olan pestisit kaynaklı oksidatif stres, son on yılda en çok odaklanan araştırma alanıdır. Literatürde pestisit maruziyetinin oksidatif stres parametresi aracılı toksisiteyi indüklemesine ilişkin farklı çalışmalar mevcuttur. Pestisit kaynaklı oksidatif stres seviyesi, memeli sistemlerinin biyokimyasal özelliklerine bağlıdır. İmidakloprid, güvenli olduğu düşünülen, yaygın olarak kullanılan bir neonikotinoid pestisittir; ancak oksidatif stres parametrelerinde değişikliklere neden olabileceği farklı çalışmalarda bildirilmiştir.Gereç ve Yöntemler: Bu çalışmada doza ve zamana bağımlı olarak imidaklopridin L-929 fibroblast hücrelerinde AChE, laktat dehidrogenaz (LDH) ve glutatyon (GSH) düzeyleri üzerine etkisini inceledik. 1-500 μg imidakloprid dozlarının asetilkolinesteraz, glutatyon ve laktat dehidrojenaz düzeyleri üzerine etkileri araştırıldı. Bulgular: 250 ve 500 ng dozda imidaklopridin LDH seviyelerini, kontrol grubuna kıyasla anlamlı olarak artırdığı tespit edildi. GSH seviyelesinin dozdan bağımsız olarak 500 ng imidakloprid dozunda kontrol grubuna kıyasla anlamlı olarak azaldığı tespit edildi. Asetilkolinesteraz seviyeleri arasında anlamlı bir fark gözlenmedi. Sonuç: Bu sonuçlara göre yüksek doz imidakloprid maruziyeti fibroblast hücrelerinde oksidatif stres parametrelerini uyarabileceği gözlemlenmiştir.
Anahtar Kelime: Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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| APA | SEVIM Ç, TAGHİZADEHGHALEHJOUGHİ A, Kara M (2020). In Vitro Investigation of the Effects of Imidacloprid on AChE, LDH, and GSH Levels in the L-929 Fibroblast Cell Line. , 506 - 510. 10.4274/tjps.galenos.2019.15807 |
| Chicago | SEVIM ÇIGDEM,TAGHİZADEHGHALEHJOUGHİ Ali,Kara Mehtap In Vitro Investigation of the Effects of Imidacloprid on AChE, LDH, and GSH Levels in the L-929 Fibroblast Cell Line. (2020): 506 - 510. 10.4274/tjps.galenos.2019.15807 |
| MLA | SEVIM ÇIGDEM,TAGHİZADEHGHALEHJOUGHİ Ali,Kara Mehtap In Vitro Investigation of the Effects of Imidacloprid on AChE, LDH, and GSH Levels in the L-929 Fibroblast Cell Line. , 2020, ss.506 - 510. 10.4274/tjps.galenos.2019.15807 |
| AMA | SEVIM Ç,TAGHİZADEHGHALEHJOUGHİ A,Kara M In Vitro Investigation of the Effects of Imidacloprid on AChE, LDH, and GSH Levels in the L-929 Fibroblast Cell Line. . 2020; 506 - 510. 10.4274/tjps.galenos.2019.15807 |
| Vancouver | SEVIM Ç,TAGHİZADEHGHALEHJOUGHİ A,Kara M In Vitro Investigation of the Effects of Imidacloprid on AChE, LDH, and GSH Levels in the L-929 Fibroblast Cell Line. . 2020; 506 - 510. 10.4274/tjps.galenos.2019.15807 |
| IEEE | SEVIM Ç,TAGHİZADEHGHALEHJOUGHİ A,Kara M "In Vitro Investigation of the Effects of Imidacloprid on AChE, LDH, and GSH Levels in the L-929 Fibroblast Cell Line." , ss.506 - 510, 2020. 10.4274/tjps.galenos.2019.15807 |
| ISNAD | SEVIM, ÇIGDEM vd. "In Vitro Investigation of the Effects of Imidacloprid on AChE, LDH, and GSH Levels in the L-929 Fibroblast Cell Line". (2020), 506-510. https://doi.org/10.4274/tjps.galenos.2019.15807 |
| APA | SEVIM Ç, TAGHİZADEHGHALEHJOUGHİ A, Kara M (2020). In Vitro Investigation of the Effects of Imidacloprid on AChE, LDH, and GSH Levels in the L-929 Fibroblast Cell Line. Turkish Journal of Pharmaceutical Sciences, 17(5), 506 - 510. 10.4274/tjps.galenos.2019.15807 |
| Chicago | SEVIM ÇIGDEM,TAGHİZADEHGHALEHJOUGHİ Ali,Kara Mehtap In Vitro Investigation of the Effects of Imidacloprid on AChE, LDH, and GSH Levels in the L-929 Fibroblast Cell Line. Turkish Journal of Pharmaceutical Sciences 17, no.5 (2020): 506 - 510. 10.4274/tjps.galenos.2019.15807 |
| MLA | SEVIM ÇIGDEM,TAGHİZADEHGHALEHJOUGHİ Ali,Kara Mehtap In Vitro Investigation of the Effects of Imidacloprid on AChE, LDH, and GSH Levels in the L-929 Fibroblast Cell Line. Turkish Journal of Pharmaceutical Sciences, vol.17, no.5, 2020, ss.506 - 510. 10.4274/tjps.galenos.2019.15807 |
| AMA | SEVIM Ç,TAGHİZADEHGHALEHJOUGHİ A,Kara M In Vitro Investigation of the Effects of Imidacloprid on AChE, LDH, and GSH Levels in the L-929 Fibroblast Cell Line. Turkish Journal of Pharmaceutical Sciences. 2020; 17(5): 506 - 510. 10.4274/tjps.galenos.2019.15807 |
| Vancouver | SEVIM Ç,TAGHİZADEHGHALEHJOUGHİ A,Kara M In Vitro Investigation of the Effects of Imidacloprid on AChE, LDH, and GSH Levels in the L-929 Fibroblast Cell Line. Turkish Journal of Pharmaceutical Sciences. 2020; 17(5): 506 - 510. 10.4274/tjps.galenos.2019.15807 |
| IEEE | SEVIM Ç,TAGHİZADEHGHALEHJOUGHİ A,Kara M "In Vitro Investigation of the Effects of Imidacloprid on AChE, LDH, and GSH Levels in the L-929 Fibroblast Cell Line." Turkish Journal of Pharmaceutical Sciences, 17, ss.506 - 510, 2020. 10.4274/tjps.galenos.2019.15807 |
| ISNAD | SEVIM, ÇIGDEM vd. "In Vitro Investigation of the Effects of Imidacloprid on AChE, LDH, and GSH Levels in the L-929 Fibroblast Cell Line". Turkish Journal of Pharmaceutical Sciences 17/5 (2020), 506-510. https://doi.org/10.4274/tjps.galenos.2019.15807 |
