Functional Variables of Bull Sperm Associated with Cryotolerance

Alicia GILMORE, (Mississippi State University, Department of Animal and Dairy Sciences, Mississippi State, MS, USA)
Mustafa HITIT, (Mississippi State University, Department of Animal and Dairy Sciences, Mississippi State, MS, USA)
Muhammet Rasit UGUR, (Mississippi State University, Department of Animal and Dairy Sciences, Mississippi State, MS, USA)
Thu Tran Nhat DINH, (Mississippi State University, Department of Animal and Dairy Sciences, Mississippi State, MS, USA)
Wei TAN, (Mississippi State University, Department of Basic Sciences, Mississippi State, MS USA)
Dean JOUSAN, (Mississippi State University, Department of Animal and Dairy Sciences, Mississippi State, MS, USA)
Molly NICODEMUS, (Mississippi State University, Department of Animal and Dairy Sciences, Mississippi State, MS, USA)
Einko TOPPER, (Alta Genetics, Inc. Watertown, WI, USA)
Abdullah KAYA, (Selçuk Üniversitesi, Veteriner Fakültesi, Suni Tohumlama ve Üreme Anabilim Dalı, Konya, Türkiye)
Erdogan MEMILI (Mississippi State University, Department of Animal and Dairy Sciences, Mississippi State, MS, USA)
Kafkas Üniversitesi Veteriner Fakültesi Dergisi
6 1
Yıl: 2021 Cilt: 27 Sayı: 3 Sayfa Aralığı: 371 - 379 Metin Dili: İngilizce DOI: 10.9775/kvfd.2021.25653 İndeks Tarihi: 04-10-2021

Functional Variables of Bull Sperm Associated with Cryotolerance

Öz:
The objective of this study was to ascertain sperm population and cellular characteristics as well as total antioxidant capacity in spermatozoafrom Holstein bulls with Good (11 bulls) and Poor (5 bulls) cryotolerance. Post-thaw sperm kinetics were evaluated using CASA, membraneintegrity was assessed via HOS test, and DNA fragmentation was measured using the HaloSperm kit. Data were analyzed using principalcomponent analysis. The spermatozoa from Good bulls had a higher number of cells with intact membranes (P=0.029), non-fragmented DNA(P=0.018), and post-thaw viability (P<0.001) compared to sperm cells from Poor cryotolerance bulls. Sperm cells from Good bulls also had afaster average path velocity (P=0.017) and straight-line velocity (P=0.036), along with a greater distance average path (P=0.006) and distancestraight line (P=0.011). However, total antioxidant capacity, number of live cells, and other kinetic parameters between spermatozoa fromGood and Poor groups were not diff erent. There is no one specific sperm function variable alone that can accurately predict cryotolerance ofbull spermatozoa, and thus, a combination of sperm cell attributes and kinematics needs to be utilized by the AI industry in diff erentiatingbetween freezability of spermatozoa between bulls.
Anahtar Kelime:

Boğa Sperminin Kriyotolerans İle İlişkili Fonksiyonel Değişkenleri

Öz:
Bu çalışmanın amacı, iyi (11 boğa) ve zayıf (5 boğa) kriyotoleransa sahip Holstein boğalardan sperm popülasyonun ve hücresel özelliklerinin yanı sıra toplam antioksidan kapasitesini belirlemektir. Dondurma çözdürme sonrası sperm kinetiği CASA kullanılarak değerlendirildi, membran bütünlüğü HOS testi ile değerlendirildi ve DNA fragmantasyonu HaloSperm kiti kullanılarak ölçüldü. Veriler, temel bileşen analizi kullanılarak değerlendirildi (PCA). İyi kriyotoleransa sahip boğalardan elde edilen spermatozoa, zayıf kriyotoleranslı boğalardan alınan sperme kıyasla daha fazla sayıda intakt membran (P=0.029), fragmente olmamış DNA’ya (P=0.018) ve dondurma çözdürme sonrası canlılığa (P<0.001) sahip hücre bulunmaktadır. İyi kriyotoleransa sahip boğa sperm hücreleri daha fazla ortalama yol (P=0.017) ve ileri doğru doğrusal hareket hızına sahiptir (P=0.036), bunula birlikte daha büyük bir ortalama mesafe hızı (P=0.006) ve ileri doğru doğrusal mesafe hızına (P=0.011) sahiptir. Ancak, toplam antioksidan kapasitesi, canlı hücre sayısı ve kinetik parametreler iyi ve zayıf kriyotoleransa sahip spermatozoa grupları arasında farklı değildir. Boğa spermatozoanın kriyotoleransını doğru bir şekilde tahmin edebilecek tek bir spesifik sperm fonksiyon değişkeni yoktur ve bu nedenle, sperm hücresi özelliklerinin ve kinematiklerin bir kombinasyonunun, boğalar arasındaki spermlerin dondurulabilirliğini ayırt etmede suni tohumlama endüstrisi tarafından kullanılması önerilmektedir
Anahtar Kelime:

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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APA Gilmore A, Hitit M, Ugur M, Dinh T, TAN W, Jousan F, Nicodemus M, TOPPER E, Kaya A, Memili E (2021). Functional Variables of Bull Sperm Associated with Cryotolerance. , 371 - 379. 10.9775/kvfd.2021.25653
Chicago Gilmore Alicia,Hitit Mustafa,Ugur Muhammet Rasit,Dinh Thu,TAN Wei,Jousan Frank Dean,Nicodemus Molly,TOPPER Einko,Kaya Abdullah,Memili Erdogan Functional Variables of Bull Sperm Associated with Cryotolerance. (2021): 371 - 379. 10.9775/kvfd.2021.25653
MLA Gilmore Alicia,Hitit Mustafa,Ugur Muhammet Rasit,Dinh Thu,TAN Wei,Jousan Frank Dean,Nicodemus Molly,TOPPER Einko,Kaya Abdullah,Memili Erdogan Functional Variables of Bull Sperm Associated with Cryotolerance. , 2021, ss.371 - 379. 10.9775/kvfd.2021.25653
AMA Gilmore A,Hitit M,Ugur M,Dinh T,TAN W,Jousan F,Nicodemus M,TOPPER E,Kaya A,Memili E Functional Variables of Bull Sperm Associated with Cryotolerance. . 2021; 371 - 379. 10.9775/kvfd.2021.25653
Vancouver Gilmore A,Hitit M,Ugur M,Dinh T,TAN W,Jousan F,Nicodemus M,TOPPER E,Kaya A,Memili E Functional Variables of Bull Sperm Associated with Cryotolerance. . 2021; 371 - 379. 10.9775/kvfd.2021.25653
IEEE Gilmore A,Hitit M,Ugur M,Dinh T,TAN W,Jousan F,Nicodemus M,TOPPER E,Kaya A,Memili E "Functional Variables of Bull Sperm Associated with Cryotolerance." , ss.371 - 379, 2021. 10.9775/kvfd.2021.25653
ISNAD Gilmore, Alicia vd. "Functional Variables of Bull Sperm Associated with Cryotolerance". (2021), 371-379. https://doi.org/10.9775/kvfd.2021.25653
APA Gilmore A, Hitit M, Ugur M, Dinh T, TAN W, Jousan F, Nicodemus M, TOPPER E, Kaya A, Memili E (2021). Functional Variables of Bull Sperm Associated with Cryotolerance. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 27(3), 371 - 379. 10.9775/kvfd.2021.25653
Chicago Gilmore Alicia,Hitit Mustafa,Ugur Muhammet Rasit,Dinh Thu,TAN Wei,Jousan Frank Dean,Nicodemus Molly,TOPPER Einko,Kaya Abdullah,Memili Erdogan Functional Variables of Bull Sperm Associated with Cryotolerance. Kafkas Üniversitesi Veteriner Fakültesi Dergisi 27, no.3 (2021): 371 - 379. 10.9775/kvfd.2021.25653
MLA Gilmore Alicia,Hitit Mustafa,Ugur Muhammet Rasit,Dinh Thu,TAN Wei,Jousan Frank Dean,Nicodemus Molly,TOPPER Einko,Kaya Abdullah,Memili Erdogan Functional Variables of Bull Sperm Associated with Cryotolerance. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, vol.27, no.3, 2021, ss.371 - 379. 10.9775/kvfd.2021.25653
AMA Gilmore A,Hitit M,Ugur M,Dinh T,TAN W,Jousan F,Nicodemus M,TOPPER E,Kaya A,Memili E Functional Variables of Bull Sperm Associated with Cryotolerance. Kafkas Üniversitesi Veteriner Fakültesi Dergisi. 2021; 27(3): 371 - 379. 10.9775/kvfd.2021.25653
Vancouver Gilmore A,Hitit M,Ugur M,Dinh T,TAN W,Jousan F,Nicodemus M,TOPPER E,Kaya A,Memili E Functional Variables of Bull Sperm Associated with Cryotolerance. Kafkas Üniversitesi Veteriner Fakültesi Dergisi. 2021; 27(3): 371 - 379. 10.9775/kvfd.2021.25653
IEEE Gilmore A,Hitit M,Ugur M,Dinh T,TAN W,Jousan F,Nicodemus M,TOPPER E,Kaya A,Memili E "Functional Variables of Bull Sperm Associated with Cryotolerance." Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 27, ss.371 - 379, 2021. 10.9775/kvfd.2021.25653
ISNAD Gilmore, Alicia vd. "Functional Variables of Bull Sperm Associated with Cryotolerance". Kafkas Üniversitesi Veteriner Fakültesi Dergisi 27/3 (2021), 371-379. https://doi.org/10.9775/kvfd.2021.25653
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23-04-2024 19:29

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