TY  - JOUR
TI  - Functional Variables of Bull Sperm Associated with Cryotolerance
AB  - The objective of this study was to ascertain sperm population and cellular characteristics as well as total antioxidant capacity in spermatozoafrom Holstein bulls with Good (11 bulls) and Poor (5 bulls) cryotolerance. Post-thaw sperm kinetics were evaluated using CASA, membraneintegrity was assessed via HOS test, and DNA fragmentation was measured using the HaloSperm kit. Data were analyzed using principalcomponent analysis. The spermatozoa from Good bulls had a higher number of cells with intact membranes (P=0.029), non-fragmented DNA(P=0.018), and post-thaw viability (P<0.001) compared to sperm cells from Poor cryotolerance bulls. Sperm cells from Good bulls also had afaster average path velocity (P=0.017) and straight-line velocity (P=0.036), along with a greater distance average path (P=0.006) and distancestraight line (P=0.011). However, total antioxidant capacity, number of live cells, and other kinetic parameters between spermatozoa fromGood and Poor groups were not diff erent. There is no one specific sperm function variable alone that can accurately predict cryotolerance ofbull spermatozoa, and thus, a combination of sperm cell attributes and kinematics needs to be utilized by the AI industry in diff erentiatingbetween freezability of spermatozoa between bulls.
AU  - Nicodemus, Molly
AU  - Memili, Erdogan
AU  - Ugur, Muhammet Rasit
AU  - Dinh, Thu
AU  - Kaya, Abdullah
AU  - TOPPER, Einko
AU  - Gilmore, Alicia
AU  - TAN, Wei
AU  - Hitit, Mustafa
AU  - Jousan, Frank Dean
DO  - 10.9775/kvfd.2021.25653
PY  - 2021
JO  - Kafkas Üniversitesi Veteriner Fakültesi Dergisi
VL  - 27
IS  - 3
SN  - 1300-6045
SP  - 371
EP  - 379
DB  - TRDizin
UR  - http://search/yayin/detay/447876
ER  -