Background: In this study we aimed to investigate burnout levels of the emergency employees during the pandemic period and the related factors. Materials and Methods: 140 emergency service employees working in two large pandemic hospitals in Samsun province were included in the study. Maslach Burnout Inventory was performed on the participants. Results: A total of 140 people participated in the study, wherein 55% are males and 45% are females, 55.8% are married, and 40.7% of them are in the age group of 18–27 years and 30% are 28-37 years. In occupational examination of participants, nurses rank first at the rate of 32.1% and practitioners rank second at 28.5%. In addition, 40.7% of participants got infected with COVID-19 during the pandemic. Maslach burnout inventory was compared by demographic data, occupation, and medical history of COVID -19 of the participants. In consequence of the comparison of Maslach burnout inventory by demographic data, occupation, and COVID19 infection medical history of participants, statistically significant difference was found between gender (p = 0.003), educational backgrounds (p = 0.006), and COVID-19 disease history (p = 0.024) in the EE subscale, between gender (p = 0.038) and educational backgrounds (p = 0.003) in the desensitization subscale, and between genders (p = 0.007) in the personal success subscale. In addition, multivariate linear regression analysis showed that gender (β = 0.247, p = 0.003) and COVID-19 disease history (β = 0.219, p = 0.011) were independent predictors for emotional exhaustion. Conclusions: In this study, we found that gender, educational background, and history of COVID-19 infection affect burnout. Also; We found that history of COVID-19 infection and gender are an independent predictor of emotional exhaustion.