Internal transcribed spacer (ITS) polymorphism in the wild Primula (Primulaceae) taxa of Turkey

TERZİOĞLU, SALİH; GÜLTEPE, MUTLU; BELDUZ, Ali Osman; ÇOŞKUNÇELEBİ, KAMİL; UZUNE, Uğur

Internal transcribed spacer (ITS) polymorphism in the wild Primula (Primulaceae) taxa of Turkey

Mutlu GÜLTEPE, (Karadeniz Teknik Üniversitesi, Fen Edebiyat Fakültesi, Biyoloji Bölümü, Trabzon, Türkiye)

Uğur UZUNE, (Texas A&M Univercity Department, of Plant Pathology Microbiology, College Station, TX 77843, USA)

KAMİL ÇOŞKUNÇELEBİ, (Karadeniz Teknik Üniversitesi, Fen Edebiyat Fakültesi, Biyoloji Bölümü, Trabzon, Türkiye)

Ali Osman BELDÜZ, (Karadeniz Teknik Üniversitesi, Fen Edebiyat Fakültesi, Biyoloji Bölümü, Trabzon, Türkiye)

Salih TERZİOĞLU (Karadeniz Teknik Üniversitesi, Orman Fakültesi, Trabzon, Türkiye)

Turkish Journal of Botany

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Yıl: 2010 Cilt: 34 Sayı: 3 Sayfa Aralığı: 147 - 157 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022

Internal transcribed spacer (ITS) polymorphism in the wild Primula (Primulaceae) taxa of Turkey

Öz:

Bu çalışmada, morfolojik olarak birbirine oldukça benzeyen 10 doğal Primula L. (Primulaceae) taksonuna ait 23 populasyon nrDNA ITS bölgeleri bakımından incelenmiştir. Bitki örnekleri 2005-2006 vejetasyon döneminde Anadolu’nun farklı coğrafik alanlarından toplanmıştır. Her populasyonun genomik DNA’ları sağlıklı yapraklardan elde edilmiştir. ITS bölgeleri evrensel oligonükleotidler kullanılarak PCR yardımıyla elde edilmiş ve baz dizin analizleri yapılmıştır. Primula taksonları arasındaki filogenetik ilişkiyi belirlemek için Neighbour-Joining (NJ) ve Maksimum Parsimoni (MP) ağaçları çizilmiştir. Populasyonlara ait baz dizin verilerinin hizalanmasıyla 724 karakterlik bir veri matriksi elde edilmiş ve divergens değerlerinin de % 0,0-20,9 arasında değiştiği bulunmuştur. ITS baz dizinleri 23 örnekte akrabalık ilişkilerini açıklamada yeterli veri sağlamaktadır. Sonuç olarak, NJ ve MP analizlerinden elde edilen dendrogramların altcins seviyesinde geleneksel taksonomik verilerle uyumlu olduğu bulunmuştur.

Anahtar Kelime: DNA Primula genetik polimorfizm yapraklar Türkiye filogeni takson

Konular: Biyoloji

Türkiye’de doğal olarak yayılış gösteren Primula (Primulaceae) taksonlarının ITS polimorfizmi

Öz:

Twenty-three populations belonging to 10 wild Primula L. (Primulaceae) taxa of Turkey, some of which are morphologically quite similar, were investigated based on nrDNA ITS regions. The plant materials were collected from different geographical areas of Anatolia in vegetation periods of 2005 and 2006. Total genomic DNAs were isolated from the healthy leaves of each population. The entire ITS regions of the populations were amplified by universal primers with the aid of a polymerase chain reaction (PCR), and then the PCR products were sequenced. Neighbour-Joining (NJ) and Maximum Parsimony (MP) trees were constructed in order to identify the relationships among Primula taxa. According to ITS data, 724 characters were determined among the aligned sequences of the populations and the divergence values were found to be between 0.0% and 20.9%. ITS sequences from 23 specimens provided a number of variable and sufficient characters to explore the relationships. As a result, it was determined that the dendrograms obtained by NJ and MP analysis are concordant with the traditional taxonomical order at subgeneric level.

Anahtar Kelime: genetic polymorphism leaves Turkey phylogeny taxa DNA Primula

Konular: Biyoloji

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	GÜLTEPE M, UZUNE U, ÇOŞKUNÇELEBİ K, BELDUZ A, TERZİOĞLU S (2010). Internal transcribed spacer (ITS) polymorphism in the wild Primula (Primulaceae) taxa of Turkey. , 147 - 157.
Chicago	GÜLTEPE MUTLU,UZUNE Uğur,ÇOŞKUNÇELEBİ KAMİL,BELDUZ Ali Osman,TERZİOĞLU SALİH Internal transcribed spacer (ITS) polymorphism in the wild Primula (Primulaceae) taxa of Turkey. (2010): 147 - 157.
MLA	GÜLTEPE MUTLU,UZUNE Uğur,ÇOŞKUNÇELEBİ KAMİL,BELDUZ Ali Osman,TERZİOĞLU SALİH Internal transcribed spacer (ITS) polymorphism in the wild Primula (Primulaceae) taxa of Turkey. , 2010, ss.147 - 157.
AMA	GÜLTEPE M,UZUNE U,ÇOŞKUNÇELEBİ K,BELDUZ A,TERZİOĞLU S Internal transcribed spacer (ITS) polymorphism in the wild Primula (Primulaceae) taxa of Turkey. . 2010; 147 - 157.
Vancouver	GÜLTEPE M,UZUNE U,ÇOŞKUNÇELEBİ K,BELDUZ A,TERZİOĞLU S Internal transcribed spacer (ITS) polymorphism in the wild Primula (Primulaceae) taxa of Turkey. . 2010; 147 - 157.
IEEE	GÜLTEPE M,UZUNE U,ÇOŞKUNÇELEBİ K,BELDUZ A,TERZİOĞLU S "Internal transcribed spacer (ITS) polymorphism in the wild Primula (Primulaceae) taxa of Turkey." , ss.147 - 157, 2010.
ISNAD	GÜLTEPE, MUTLU vd. "Internal transcribed spacer (ITS) polymorphism in the wild Primula (Primulaceae) taxa of Turkey". (2010), 147-157.

APA	GÜLTEPE M, UZUNE U, ÇOŞKUNÇELEBİ K, BELDUZ A, TERZİOĞLU S (2010). Internal transcribed spacer (ITS) polymorphism in the wild Primula (Primulaceae) taxa of Turkey. Turkish Journal of Botany, 34(3), 147 - 157.
Chicago	GÜLTEPE MUTLU,UZUNE Uğur,ÇOŞKUNÇELEBİ KAMİL,BELDUZ Ali Osman,TERZİOĞLU SALİH Internal transcribed spacer (ITS) polymorphism in the wild Primula (Primulaceae) taxa of Turkey. Turkish Journal of Botany 34, no.3 (2010): 147 - 157.
MLA	GÜLTEPE MUTLU,UZUNE Uğur,ÇOŞKUNÇELEBİ KAMİL,BELDUZ Ali Osman,TERZİOĞLU SALİH Internal transcribed spacer (ITS) polymorphism in the wild Primula (Primulaceae) taxa of Turkey. Turkish Journal of Botany, vol.34, no.3, 2010, ss.147 - 157.
AMA	GÜLTEPE M,UZUNE U,ÇOŞKUNÇELEBİ K,BELDUZ A,TERZİOĞLU S Internal transcribed spacer (ITS) polymorphism in the wild Primula (Primulaceae) taxa of Turkey. Turkish Journal of Botany. 2010; 34(3): 147 - 157.
Vancouver	GÜLTEPE M,UZUNE U,ÇOŞKUNÇELEBİ K,BELDUZ A,TERZİOĞLU S Internal transcribed spacer (ITS) polymorphism in the wild Primula (Primulaceae) taxa of Turkey. Turkish Journal of Botany. 2010; 34(3): 147 - 157.
IEEE	GÜLTEPE M,UZUNE U,ÇOŞKUNÇELEBİ K,BELDUZ A,TERZİOĞLU S "Internal transcribed spacer (ITS) polymorphism in the wild Primula (Primulaceae) taxa of Turkey." Turkish Journal of Botany, 34, ss.147 - 157, 2010.
ISNAD	GÜLTEPE, MUTLU vd. "Internal transcribed spacer (ITS) polymorphism in the wild Primula (Primulaceae) taxa of Turkey". Turkish Journal of Botany 34/3 (2010), 147-157.