DNA extraction protocol from Brown Algae
Yıl: 2010 Cilt: 3 Sayı: 1 Sayfa Aralığı: 51 - 55 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022
DNA extraction protocol from Brown Algae
Öz: Denizel alglerden DNA izolasyonu için çeşitli yöntemler bulunmaktadır. Ancak bu yöntemler diğer canlılar için kullanılan DNA izolasyon yöntemlerinin modifikasyonlarıdır. Alglerden DNA izolasyonu talluslarında bulunan polisakkaritler ve fenolik bileşiklerden dolayı zor bir işlemdir. Bu çalışmada, Izmir Körfezi’nde toplanan kahverengi alglerden (Scytosiphon lomentaria ve Cystoseira sp., Ectocarpus sp. ) CTAB (cetiltrimethylamonium bromide) yöntemiyle DNA izolasyonu gerçekleştirilmiş ve elde edilen DNA PCR yöntemi ile çoğaltılmıştır. Bu modifiye yöntem ile moleküler amaçlı çalışmalar için uygun DNA eldesi sağlanmıştır.
Anahtar Kelime: Konular:
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Öz: There are various methods published about DNA extraction from marine algae. These methods are the modifications of several DNA extraction methods from other organisms. Extraction of DNA from seaweeds are difficult processes because of the polysaccharide and polyphenole compounds of their thallus. In this study, DNA is extracted from a brown alga (Scytosiphon lomentaria and Cystoseira sp., Ectocarpus sp. ) collected from the Bay of Izmir by using modified CTAB (cetiltrimethylamonium bromide) protocol and used in PCR analysis. This modified method was also found efficient and applicaple for other molecular purposes.
Anahtar Kelime: Konular:
Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
- Bold, H.C, Wynne M. J. 1978. Introduction to the algae: structure and reproduction. New Jersey: Prentice-Hall Inc.
- Coleman, A. W., L. J. Goff, J. R. Stein-Taylor. 1989. Algae as Experimental Systes. A. R. Liss, Inc., New York.
- Colosi, Schall 1993. CTAB miniprep DNA extraction protocol. Laboratuary Book.
- Coyer, J.A., Steller, D.L., Alberte, R.S. 1995. A field-competible method for extraction of fingerprint-quality DNA from Macrocystis pyrifera (Phaeophyceae). J. Phycol. 31: 177-180.
- Dutcher, S. K., J. Power, R. E. Galloway and M. E. Porter, 1991 Reappraisal of the genetic map of Chlamydomonas reinhardtii. J. Hered. 82: 295–301.
- Anna Godhe, Donald M. Anderson, Ann-Sofi Rehnstam-Holm. PCR amplification of microalgal DNA for sequencing and species identification: studies on fixatives and algal growth stages. Harmful Algae 4 (1): 375-382.
- John M. E. 1992. An efficient method for isolation of RNA and DNA from plantscontaining polyphenolics. Nucleic Acids Res. 20: 2381.
- Saunders, G.W. 1993. Gel purification of red aldal genomic DNA: an inexpensive and rapid method for the isolation of polymerase chain reaction- friendly DNA. J. Phycol. 29, 251-254.
- Shioda M., Murakmi-Murofusi K. 1987. Selective inhibition of DNA polymerase by polysaccharide purified from slime of Physarum polycephalum. Biochem. Biophys. Re. Commun. 146: 5279-5280.
- Sosa & Olivera, 1992. Nucleic acid extraction from seaweed tissues for polymerase chain reaction. Journal of Marine Biotechnology 5: 95-99.
- Staves M. P., J. W. LaClaire. 1985. Nuclear synchrony in Valonia macrophysa (Chlorophyta) light microscopy and flow cytometry. J. Phycol. 21: 68-81.
- Steen, S.W. 1999. Handbook for DNA isolation, RAPD-PCR and PCR-RFLP. Botanical Garden and Museum, University of Oslo.
- Vidal et al., B. Vidal, C. Pasqualini, N. Le Belle, M.C. Holland, M. Sbaihi, P. Vernier, Y. Zohar and S. Dufour, 2004. Dopamine inhibits luteinizing hormone synthesis and release in the juvenile European eel: a neuroendocrine lock for the onset of puberty, Biol. Reprod. 7: 1491–1500.
- Wattier, R.A., Prodolh, P.A., Maggs, C.A. 2000. DNA isolation protocol for red seaweed (Rhodophyta). Plant Molecular Biology Reporter 18: 275-281.
- James L. Wee, Joby Chesnick, Rose Ann Cattolico. 1992. Partıal Characterızatıon Of The Chloroplast Genome From The Chromophytıc Alga Synura Petersenıı (Synurophyceae). Journal of Phycology 29-1: 96-99.
| APA | Tuney I, SUKATAR A (2010). DNA extraction protocol from Brown Algae. , 51 - 55. |
| Chicago | Tuney Inci,SUKATAR Atakan DNA extraction protocol from Brown Algae. (2010): 51 - 55. |
| MLA | Tuney Inci,SUKATAR Atakan DNA extraction protocol from Brown Algae. , 2010, ss.51 - 55. |
| AMA | Tuney I,SUKATAR A DNA extraction protocol from Brown Algae. . 2010; 51 - 55. |
| Vancouver | Tuney I,SUKATAR A DNA extraction protocol from Brown Algae. . 2010; 51 - 55. |
| IEEE | Tuney I,SUKATAR A "DNA extraction protocol from Brown Algae." , ss.51 - 55, 2010. |
| ISNAD | Tuney, Inci - SUKATAR, Atakan. "DNA extraction protocol from Brown Algae". (2010), 51-55. |
| APA | Tuney I, SUKATAR A (2010). DNA extraction protocol from Brown Algae. Biological Diversity and Conservation, 3(1), 51 - 55. |
| Chicago | Tuney Inci,SUKATAR Atakan DNA extraction protocol from Brown Algae. Biological Diversity and Conservation 3, no.1 (2010): 51 - 55. |
| MLA | Tuney Inci,SUKATAR Atakan DNA extraction protocol from Brown Algae. Biological Diversity and Conservation, vol.3, no.1, 2010, ss.51 - 55. |
| AMA | Tuney I,SUKATAR A DNA extraction protocol from Brown Algae. Biological Diversity and Conservation. 2010; 3(1): 51 - 55. |
| Vancouver | Tuney I,SUKATAR A DNA extraction protocol from Brown Algae. Biological Diversity and Conservation. 2010; 3(1): 51 - 55. |
| IEEE | Tuney I,SUKATAR A "DNA extraction protocol from Brown Algae." Biological Diversity and Conservation, 3, ss.51 - 55, 2010. |
| ISNAD | Tuney, Inci - SUKATAR, Atakan. "DNA extraction protocol from Brown Algae". Biological Diversity and Conservation 3/1 (2010), 51-55. |
