TY  - JOUR
TI  - Development of a highly responsive leaf-based regeneration system for Peperomia species
AB  - Several species in the genus Peperomia Ruiz & Pav. have giant chloroplasts in the palisade parenchyma of their leaves. Because of this unique feature, Peperomia metallica L. Linden & Rodigas has become a valuable model plant for studying plastid biology. However, the use of Peperomia for molecular studies has been limited by the lack of efficient regeneration and transformation protocols. In this work, we aimed to develop a reliable regeneration system for Peperomia from leaf explants. Using MS medium complemented with different combinations of phytohormones, a highly efficient in vitro regeneration protocol was developed for P. metallica and P. peduncularis, another species with giant chloroplasts. Regeneration occurs through direct organogenesis from leaf discs at a very high frequency. Our protocol can provide a reliable tissue culture system for future Peperomia genetic transformation experiments.
AU  - BOCK, Ralph
AU  - AHMADABADI, Mohammad
PY  - 2010
JO  - Turkish Journal of Botany
VL  - 34
IS  - 4
SN  - 1300-008X
SP  - 329
EP  - 334
DB  - TRDizin
UR  - http://search/yayin/detay/103117
ER  -