Analysis of serum pro-Inflammatory cytokine levels after rat spinal cord ischemia/reperfusion injury and correlation with tissue damage

ÇALIŞANELLER, Tarkan; ÖZDEMİR, Özgür; ORUÇKAPTAN, Hakan; ATALAY, Başar; HASTURİK, Aşkın; ALTINÖRS, Nur

Analysis of serum pro-Inflammatory cytokine levels after rat spinal cord ischemia/reperfusion injury and correlation with tissue damage

Aşkın HASTURİK, (Başkent Üniversitesi, Tıp Fakültesi, Nöroşirürji Anabilim Dalı, Ankara, Türkiye)

Başar ATALAY, (Yeditepe Üniversitesi, Tıp Fakültesi, Nöroşirürji Anabilim Dalı, İstanbul, Türkiye)

Tarkan ÇALIŞANELLER, (Başkent Üniversitesi, Tıp Fakültesi, Konya Araştırma ve Uygulama Hastanesi, Nöroşirürji Anabilim Dalı, Konya, Türkiye)

Özgür ÖZDEMİR, (Başkent Üniversitesi, Tıp Fakültesi, Konya Araştırma ve Uygulama Hastanesi, Nöroşirürji Anabilim Dalı, Konya, Türkiye)

Hakan ORUÇKAPTAN, (Hacettepe Üniversitesi, Tıp Fakültesi, Nöroşirürji Anabilim Dalı, Ankara, Türkiye)

Nur ALTINÖRS (Başkent Üniversitesi, Tıp Fakültesi, Nöroşirürji Anabilim Dalı, Ankara, Türkiye)

Turkish Neurosurgery

3 0

Yıl: 2009 Cilt: 19 Sayı: 4 Sayfa Aralığı: 353 - 359 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022

Analysis of serum pro-Inflammatory cytokine levels after rat spinal cord ischemia/reperfusion injury and correlation with tissue damage

Öz:

AMAÇ: Rat spinal kord iskemi/reperfüzyon modeli oluşturuldu ve serum sitokin düzeyleri ve histopatolojik değişiklikler değerlendirildi. YÖNTEM ve GEREÇ: Yirmidört Sprague-Dawley rat dört deney grubuna ayrıldı. Grup A sham opereli ve Grup B spinal iskemi/reperfüzyon grubu ratlar postoperatif 24. saatte sakrifiye edildi. Grup C sham opereli ve Grup D spinal iskemi/reperfüzyon grubu ratlar postoperatif 48. saattte sakrifiye edildi. Spinal korddaki histopatolojik değişiklikler ve TNF- , IL-1ß ve IL-6 için serum sitokin düzeyleri incelendi. BULGULAR: Hem 24. saat, hem de 48. saat spinal kord iskemi/reperfüzyon gruplarında her üç proinflamatuvar sitokin düzeyleri sham opereli gruplarla karşılaştırıldığında belirgin olarak daha yüksek düzeylere eriştiler. SONUÇ: Spinal kord iskemi/reperfüzyon hasarında iflamasyon olası bir durumdur. Ancak hasarın klinik tedavisi günümüzde antiinflamatuvar tedaviyi içermemektedir. Bizim çalışmamızın sonuçları spinal kord iskemi/reperfüzyon hasarında inflamatuvar cevapların olası rol oynayabileceği hipotezini desteklemektedir. Spinal kordda iskemi/reperfüzyon hasarının etyopatogenezinde inflamasyonun rolünün tanımlanması bu ciddi durumun önlenmesi ve tedavisi için gelecek terapötik yaklaşımların gelişmesini kolaylaştırmada önemlidir.

Anahtar Kelime:

Konular: Cerrahi

Rat spinal kord iskemi/reperfüzyon hasarı sonrası proinflamatuvar sitokin düzeyleri ve doku hasarı ile ilişkisi

Öz:

AIM: A rat model of spinal cord ischemia/reperfusion was conducted and the serum cytokine levels and histopathological changes were assessed. MATERIAL and METHODS: Twenty-four male Sprague-Dawley rats were assigned into four experimental groups. Group-A (the sham operated rats) and group-B (the spinal ischemia/reperfusion group) were sacrificed at 24 hours postoperatively while group-C (the sham operated rats) and group-D (the spinal ischemia/reperfusion group) were sacrificed at 48 hours. Histopathological changes in the spinal cords and serum cytokine levels were analysed. RESULTS: All three proinflammatory cytokine levels reached significantly higher levels compared to the sham operated groups in both the 24-hour and 48-hour spinal cord ischemia/reperfusion groups. CONCLUSION: Inflammation is a plausible pathway in spinal cord ishemia/reperfusion injury. However clinical treatment of the damage does not currently include antiinflammatory therapy. The results of our study supported the hypothesis that inflammatory responses could play a possible role in the ischemia/reperfusion injury of the spinal cord. Characterization of the role of inflammation in the etiopathogenesis of ischemia/reperfusion injury to the spinal cord is important to facilitate the development of novel therapeutic approaches for prevention and/or treatment of this severe condition.

Anahtar Kelime:

Konular: Cerrahi

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	HASTURİK A, ATALAY B, ÇALIŞANELLER T, ÖZDEMİR Ö, ORUÇKAPTAN H, ALTINÖRS N (2009). Analysis of serum pro-Inflammatory cytokine levels after rat spinal cord ischemia/reperfusion injury and correlation with tissue damage. , 353 - 359.
Chicago	HASTURİK Aşkın,ATALAY Başar,ÇALIŞANELLER Tarkan,ÖZDEMİR Özgür,ORUÇKAPTAN Hakan,ALTINÖRS Nur Analysis of serum pro-Inflammatory cytokine levels after rat spinal cord ischemia/reperfusion injury and correlation with tissue damage. (2009): 353 - 359.
MLA	HASTURİK Aşkın,ATALAY Başar,ÇALIŞANELLER Tarkan,ÖZDEMİR Özgür,ORUÇKAPTAN Hakan,ALTINÖRS Nur Analysis of serum pro-Inflammatory cytokine levels after rat spinal cord ischemia/reperfusion injury and correlation with tissue damage. , 2009, ss.353 - 359.
AMA	HASTURİK A,ATALAY B,ÇALIŞANELLER T,ÖZDEMİR Ö,ORUÇKAPTAN H,ALTINÖRS N Analysis of serum pro-Inflammatory cytokine levels after rat spinal cord ischemia/reperfusion injury and correlation with tissue damage. . 2009; 353 - 359.
Vancouver	HASTURİK A,ATALAY B,ÇALIŞANELLER T,ÖZDEMİR Ö,ORUÇKAPTAN H,ALTINÖRS N Analysis of serum pro-Inflammatory cytokine levels after rat spinal cord ischemia/reperfusion injury and correlation with tissue damage. . 2009; 353 - 359.
IEEE	HASTURİK A,ATALAY B,ÇALIŞANELLER T,ÖZDEMİR Ö,ORUÇKAPTAN H,ALTINÖRS N "Analysis of serum pro-Inflammatory cytokine levels after rat spinal cord ischemia/reperfusion injury and correlation with tissue damage." , ss.353 - 359, 2009.
ISNAD	HASTURİK, Aşkın vd. "Analysis of serum pro-Inflammatory cytokine levels after rat spinal cord ischemia/reperfusion injury and correlation with tissue damage". (2009), 353-359.

APA	HASTURİK A, ATALAY B, ÇALIŞANELLER T, ÖZDEMİR Ö, ORUÇKAPTAN H, ALTINÖRS N (2009). Analysis of serum pro-Inflammatory cytokine levels after rat spinal cord ischemia/reperfusion injury and correlation with tissue damage. Turkish Neurosurgery, 19(4), 353 - 359.
Chicago	HASTURİK Aşkın,ATALAY Başar,ÇALIŞANELLER Tarkan,ÖZDEMİR Özgür,ORUÇKAPTAN Hakan,ALTINÖRS Nur Analysis of serum pro-Inflammatory cytokine levels after rat spinal cord ischemia/reperfusion injury and correlation with tissue damage. Turkish Neurosurgery 19, no.4 (2009): 353 - 359.
MLA	HASTURİK Aşkın,ATALAY Başar,ÇALIŞANELLER Tarkan,ÖZDEMİR Özgür,ORUÇKAPTAN Hakan,ALTINÖRS Nur Analysis of serum pro-Inflammatory cytokine levels after rat spinal cord ischemia/reperfusion injury and correlation with tissue damage. Turkish Neurosurgery, vol.19, no.4, 2009, ss.353 - 359.
AMA	HASTURİK A,ATALAY B,ÇALIŞANELLER T,ÖZDEMİR Ö,ORUÇKAPTAN H,ALTINÖRS N Analysis of serum pro-Inflammatory cytokine levels after rat spinal cord ischemia/reperfusion injury and correlation with tissue damage. Turkish Neurosurgery. 2009; 19(4): 353 - 359.
Vancouver	HASTURİK A,ATALAY B,ÇALIŞANELLER T,ÖZDEMİR Ö,ORUÇKAPTAN H,ALTINÖRS N Analysis of serum pro-Inflammatory cytokine levels after rat spinal cord ischemia/reperfusion injury and correlation with tissue damage. Turkish Neurosurgery. 2009; 19(4): 353 - 359.
IEEE	HASTURİK A,ATALAY B,ÇALIŞANELLER T,ÖZDEMİR Ö,ORUÇKAPTAN H,ALTINÖRS N "Analysis of serum pro-Inflammatory cytokine levels after rat spinal cord ischemia/reperfusion injury and correlation with tissue damage." Turkish Neurosurgery, 19, ss.353 - 359, 2009.
ISNAD	HASTURİK, Aşkın vd. "Analysis of serum pro-Inflammatory cytokine levels after rat spinal cord ischemia/reperfusion injury and correlation with tissue damage". Turkish Neurosurgery 19/4 (2009), 353-359.