A novel application for the cavalieri principle: A stereological and methodological study
Yıl: 2009 Cilt: 41 Sayı: 2 Sayfa Aralığı: 99 - 101 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022
A novel application for the cavalieri principle: A stereological and methodological study
Öz: Amaç. Bu çalışmada aynı büyütmede fotoğraflanmış ışık mikroskobik seri kesitler üzerinde Cavalieri prensibi uygulandı. Daha sonra bu kesitler üzerinde noktalı ölçüm gridi kullanılarak hacim hesaplanması yapıldı ve böylece Cavalieri prensibinin hızlı ve etkin olarak kullanılması amaçlandı. Gereç ve Yöntem. Bu çalışmada 10 adet sağlıklı ve erişkin Sprague Dawley cinsi sıçan kullanıldı. Bu sıçanlardan çıkarılan beyinler sistematik rastgele olarak örneklendi ve seri olarak kesildi. Beyin hacimleri ise iki farklı şekilde hesaplandı. İlk olarak beyin preparatları HP ScanJet 3400C marka scanner ile tarandı ve tarama görüntüleri bilgisayar monitörüne yansıtıldı. Daha sonra bu görüntüler üzerinde beyin hacimleri hesaplandı. İkinci olarak tüm beyin kesitleri x 10 büyütmede kamera ataçmanlı mikroskop ile fotoğraflandı ve beyin hacimleri bu mikrograflar üzerinde hesaplandı.Bulgular. Bu çalışmanın sonucunda her iki farklı yöntemle elde edilen beyin hacmi bulguları birbiriyle kıyaslandı ve bu sonuçlar arasında istatistiksel açıdan herhangi bir fark olmadığı görüldü (P>0,05; Eşleştirilmiş Örnekler t Testi).Sonuç. Neticede, seri kesitlerin scanner ile taranarak elde edilen görüntülerin bilgisayar monitörüne yansıtılmasının Cavalieri yönteminin uygulanabilirliği açısından kolay ve güvenilir olabileceğini düşünmekteyiz.
Anahtar Kelime: Konular:
Cavalieri prensibi için yeni bir uygulama: Stereolojik ve metodolojik bir çalışma
Öz: Objective. The Cavalieri principle was applied to consecutive pathology sections that were photographed at the same magnification and used to estimate tissue volumes via superimposing a point counting grid on these images. The goal of this study was to perform the Cavalieri method quickly and practically. Materials and Methods. In this study, 10 adult female Sprague Dawley rats were used. Brain tissue was removed and sampled both systematically and randomly. Brain volumes were estimated using two different methods. First, all brain slices were scanned with an HP ScanJet 3400C scanner, and their images were shown on a PC monitor. Brain volume was then calculated based on these images. Second, all brain slices were photographed in 10x magnification with a microscope camera, and brain volumes were estimated based on these micrographs.Results. There was no statistically significant difference between the volume measurements of the two techniques (P>0.05; Paired Samples t Test).Conclusion. This study demonstrates that personal computer scanning of serial tissue sections allows for easy and reliable volume determination based on the Cavalieri method.
Anahtar Kelime: Konular:
Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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| APA | ZUHAL B, ALTUNKAYNAK E, Ünal D, ÜNAL B (2009). A novel application for the cavalieri principle: A stereological and methodological study. , 99 - 101. |
| Chicago | ZUHAL BERRİN,ALTUNKAYNAK Eyüp,Ünal Deniz,ÜNAL Bünyamin A novel application for the cavalieri principle: A stereological and methodological study. (2009): 99 - 101. |
| MLA | ZUHAL BERRİN,ALTUNKAYNAK Eyüp,Ünal Deniz,ÜNAL Bünyamin A novel application for the cavalieri principle: A stereological and methodological study. , 2009, ss.99 - 101. |
| AMA | ZUHAL B,ALTUNKAYNAK E,Ünal D,ÜNAL B A novel application for the cavalieri principle: A stereological and methodological study. . 2009; 99 - 101. |
| Vancouver | ZUHAL B,ALTUNKAYNAK E,Ünal D,ÜNAL B A novel application for the cavalieri principle: A stereological and methodological study. . 2009; 99 - 101. |
| IEEE | ZUHAL B,ALTUNKAYNAK E,Ünal D,ÜNAL B "A novel application for the cavalieri principle: A stereological and methodological study." , ss.99 - 101, 2009. |
| ISNAD | ZUHAL, BERRİN vd. "A novel application for the cavalieri principle: A stereological and methodological study". (2009), 99-101. |
| APA | ZUHAL B, ALTUNKAYNAK E, Ünal D, ÜNAL B (2009). A novel application for the cavalieri principle: A stereological and methodological study. Eurasian Journal of Medicine, 41(2), 99 - 101. |
| Chicago | ZUHAL BERRİN,ALTUNKAYNAK Eyüp,Ünal Deniz,ÜNAL Bünyamin A novel application for the cavalieri principle: A stereological and methodological study. Eurasian Journal of Medicine 41, no.2 (2009): 99 - 101. |
| MLA | ZUHAL BERRİN,ALTUNKAYNAK Eyüp,Ünal Deniz,ÜNAL Bünyamin A novel application for the cavalieri principle: A stereological and methodological study. Eurasian Journal of Medicine, vol.41, no.2, 2009, ss.99 - 101. |
| AMA | ZUHAL B,ALTUNKAYNAK E,Ünal D,ÜNAL B A novel application for the cavalieri principle: A stereological and methodological study. Eurasian Journal of Medicine. 2009; 41(2): 99 - 101. |
| Vancouver | ZUHAL B,ALTUNKAYNAK E,Ünal D,ÜNAL B A novel application for the cavalieri principle: A stereological and methodological study. Eurasian Journal of Medicine. 2009; 41(2): 99 - 101. |
| IEEE | ZUHAL B,ALTUNKAYNAK E,Ünal D,ÜNAL B "A novel application for the cavalieri principle: A stereological and methodological study." Eurasian Journal of Medicine, 41, ss.99 - 101, 2009. |
| ISNAD | ZUHAL, BERRİN vd. "A novel application for the cavalieri principle: A stereological and methodological study". Eurasian Journal of Medicine 41/2 (2009), 99-101. |
