Does melatonin alleviate cold preservation injury of the liver?

Günal, Ömer; COŞKUN, Abdurrahman; YILDIRIM, Ümran; ASLANER, Arif

Does melatonin alleviate cold preservation injury of the liver?

Ömer GÜNAL, (Düzce Üniversitesi, Tıp Fakültesi, Genel Cerrahi Anabilim Dalı, Düzce, Türkiye)

Abdurrahman COŞKUN, (Düzce Üniversitesi, Tıp Fakültesi, Klinik Biyokimya Anabilim Dalı, Düzce, Türkiye)

Arif ASLANER, (Düzce Üniversitesi, Tıp Fakültesi, Genel Cerrahi Anabilim Dalı, Düzce, Türkiye)

Ümran YILDIRIM (Düzce Üniversitesi, Tıp Fakültesi, Patoloji Anabilim Dalı, Düzce, Türkiye)

Turkish Journal of Medical Sciences

1 0

Yıl: 2010 Cilt: 40 Sayı: 3 Sayfa Aralığı: 465 - 470 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022

Does melatonin alleviate cold preservation injury of the liver?

Öz:

Amaç: Bu çalışmada melatonin ilave edilen koruma solüsyonlarının karaciğer soğuk iskemi üzerindeki etkisi araştırıldı. Yöntem ve gereç: Yirmi erkek Wistar albino rat iki eşit gruba bölünerek melatonin içeren ve içermeyen University of Wisconsin solüsyonu ile muamele edildi. 2, 24, 36 ve 48. saatlerde koruma solüsyonlarından alınan numunelerde LDH, AST ve ACP aktiviteleri ölçüldü. 48. saatte doku malondialdehit, ısı şok protein (HSP 70) düzeyi ve histolojik inceleme yapıldı. Bulgular: Melatonin, kontrol grubuna göre ısı şok proteinini anlamlı derecede indükliyerek enzim artışını ve lipid peroksidasyonunu azaltı (P < 0,05). Histolojik incelemede melatonin içeren University of Wisconsin solüsyonunun Kupffer hücre aktivasyonunu ve inflamasyonu anlamlı düzeyde önlediği belirlendi (P < 0,05).Sonuç: University of Wisconsin gibi koruma solüsyonlarına melatoninin ilave edilmesi 48. saate kadar dokuyu soğuk iskemi hasarından koruyabilir.

Anahtar Kelime:

Konular: Cerrahi

Melatonin soğuk iskeminin karaciğer üzerındeki etkisini azaltıyormu?

Öz:

Aim: To investigate the effect of melatonin added to preservation solution on liver cold ischemia. Materials and methods: Twenty male Wistar albino rats were divided equally into groups treated with University of Wisconsin (UW) solution with and without melatonin. The LDH, AST, and ACP activities of the preservation solutions were determined in samples collected at 2, 24, 36, and 48 h. Tissue malondialdehyde and heat shock protein (HSP 70) levels were also measured and a histological examination was performed at 48 h.Results:Melatonin prevented enzyme elevation, induced heat shock protein (HSP 70), and decreased lipid peroxidation effectively in comparison to the control group (P < 0.05). The histological examination revealed that UW solution containing melatonin prevented Kupffer cell activation and inflammation significantly (P < 0.05). Conclusion:Melatonin in preservation solutions such as UW solution may protect tissue effectively from damage during cold ischemia for up to 48 h.

Anahtar Kelime:

Konular: Cerrahi

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	Günal Ö, COŞKUN A, ASLANER A, YILDIRIM Ü (2010). Does melatonin alleviate cold preservation injury of the liver?. , 465 - 470.
Chicago	Günal Ömer,COŞKUN Abdurrahman,ASLANER Arif,YILDIRIM Ümran Does melatonin alleviate cold preservation injury of the liver?. (2010): 465 - 470.
MLA	Günal Ömer,COŞKUN Abdurrahman,ASLANER Arif,YILDIRIM Ümran Does melatonin alleviate cold preservation injury of the liver?. , 2010, ss.465 - 470.
AMA	Günal Ö,COŞKUN A,ASLANER A,YILDIRIM Ü Does melatonin alleviate cold preservation injury of the liver?. . 2010; 465 - 470.
Vancouver	Günal Ö,COŞKUN A,ASLANER A,YILDIRIM Ü Does melatonin alleviate cold preservation injury of the liver?. . 2010; 465 - 470.
IEEE	Günal Ö,COŞKUN A,ASLANER A,YILDIRIM Ü "Does melatonin alleviate cold preservation injury of the liver?." , ss.465 - 470, 2010.
ISNAD	Günal, Ömer vd. "Does melatonin alleviate cold preservation injury of the liver?". (2010), 465-470.

APA	Günal Ö, COŞKUN A, ASLANER A, YILDIRIM Ü (2010). Does melatonin alleviate cold preservation injury of the liver?. Turkish Journal of Medical Sciences, 40(3), 465 - 470.
Chicago	Günal Ömer,COŞKUN Abdurrahman,ASLANER Arif,YILDIRIM Ümran Does melatonin alleviate cold preservation injury of the liver?. Turkish Journal of Medical Sciences 40, no.3 (2010): 465 - 470.
MLA	Günal Ömer,COŞKUN Abdurrahman,ASLANER Arif,YILDIRIM Ümran Does melatonin alleviate cold preservation injury of the liver?. Turkish Journal of Medical Sciences, vol.40, no.3, 2010, ss.465 - 470.
AMA	Günal Ö,COŞKUN A,ASLANER A,YILDIRIM Ü Does melatonin alleviate cold preservation injury of the liver?. Turkish Journal of Medical Sciences. 2010; 40(3): 465 - 470.
Vancouver	Günal Ö,COŞKUN A,ASLANER A,YILDIRIM Ü Does melatonin alleviate cold preservation injury of the liver?. Turkish Journal of Medical Sciences. 2010; 40(3): 465 - 470.
IEEE	Günal Ö,COŞKUN A,ASLANER A,YILDIRIM Ü "Does melatonin alleviate cold preservation injury of the liver?." Turkish Journal of Medical Sciences, 40, ss.465 - 470, 2010.
ISNAD	Günal, Ömer vd. "Does melatonin alleviate cold preservation injury of the liver?". Turkish Journal of Medical Sciences 40/3 (2010), 465-470.