Phenolic compounds and antioxidant and antimicrobial properties of Helichrysum species collected from eastern Anatolia, Turkey

ALBAYRAK, SEVİL; AKSOY, Ahmet; SAGDIC, OSMAN; Budak, Ümit

Phenolic compounds and antioxidant and antimicrobial properties of Helichrysum species collected from eastern Anatolia, Turkey

Sevil ALBAYRAK, (Erciyes Üniversitesi, Fen Edebiyat Fakültesi, Biyoloji Bölümü, Kayseri, Türkiye)

Ahmet AKSOY, (Erciyes Üniversitesi, Fen Edebiyat Fakültesi, Biyoloji Bölümü, Kayseri, Türkiye)

Osman SAĞDIÇ, (Erciyes Üniversitesi, Kayseri, Türkiye)

Ümit BUDAK (Bozok Üniversitesi, Fen Edebiyat Fakültesi, Biyoloji Bölümü, Yozgat, Türkiye)

Turkish Journal of Biology

7 0

Yıl: 2010 Cilt: 34 Sayı: 4 Sayfa Aralığı: 463 - 473 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022

Phenolic compounds and antioxidant and antimicrobial properties of Helichrysum species collected from eastern Anatolia, Turkey

Öz:

Helichrysum Mill. (Asteraceae) cinsinden dört bitkinin (H. arenarium (L.) Moench subsp. erzincanicum Davis & Kupicha, H. arenarium (L.)Moench subsp. rubicundum (C.Koch.) Davis & Kupicha, H. armenium DC. subsp. araxinum (Kirp.) Takht ve H. plicatum DC. subsp. pseudoplicatum (Nab.) Davis & Kupicha) metanollü özütlerinin antioksidan aktiviteleri DPPH (2,2-difenil-1-pikrilhidrazil) radikal süpürücü ve fosfomolibdenyum analizlerini içeren tamamlayıcı iki test sistemi tarafından araştırılmıştır. Fosfomolibdenyum analizinde H. plicatum subsp. pseudoplicatum en yüksek antioksidan aktivite (161,79 ± 0,3 mg askorbik asit eşiti/g özüt) göstermiştir. H. arenarium subsp. erzincanicum 23,03 μg/mL IC50 değeri ile en yüksek serbest radikal süpürücü aktivite göstermiştir. Özütlerin toplam fenolik içerikleri 71,81 ile 144,50 mg gallic acid/g kuru özüt arasındadır. HPLC analizi ile özütlerdeki başlıca bileşenlerin klorogenik asit, apigenin-7-glikozid ve apigenin olduğu bulunmuştur. Antimikrobiyal aktivite agar difüzyon analizi ile araştırılmıştır. Metanollü özütler A. hydrophila, B. brevis, B. cereus, K. pneumoniae, P. aeruginosa ve S. aureus ATCC 29213’e karşı antibakteriyel aktiviteye sahiptir. Fakat E. coli, M. morganii, M. smegmatis, P. mirabilis, Y. enterocolitica ve S. cerevisiae’ya karşı aktiviteye sahip olmadığı bulunmuştur.

Anahtar Kelime: fenolik bileşikler antimikrobiyal özellikler metanol Helichrysum tıbbi bitkiler bitki özütü antimikrobiyal aktivite antioksidanlar

Konular: Biyoloji

Türkiye, doğu Anadolu’dan toplanan Helichrysum türlerinin fenolik bileşik, antioksidan ve antimikrobiyal özellikleri

Öz:

The antioxidant activity of the methanolic extracts of 4 plants from genus Helichrysum Mill. (Asteraceae) [H. arenarium (L.)Moench subsp. erzincanicum Davis & Kupicha, H. arenarium (L.)Moench subsp. rubicundum (C.Koch.) Davis & Kupicha, H. armenium DC. subsp. araxinum (Kirp.) Takht., and H. plicatum DC. subsp. pseudoplicatum (Nab.) Davis & Kupicha] was investigated with 2 complementary test systems, DPPH (2,2-diphenyl-1-picrylhydrazyl) radical scavenging and phosphomolybdenum assay. H. plicatum subsp. pseudoplicatum showed the highest level of total antioxidant activity (161.79 ± 0.3 mg ascorbic acid equivalent/g extract) in the phosphomolybdenum assay. H. arenarium subsp. erzincanicum showed the highest level of free radical scavenging activity, with an IC50 value of 23.03 μg/mL. The total phenolic contents of the extracts ranged from 71.81 to 144.50 mg gallic acid/g dry extract. Chlorogenic acid, apigenin-7-glucoside, and apigenin were found by HPLC analysis to be the main components in the extracts. Antimicrobial activity was investigated with the agar diffusion method. The methanolic extracts had antibacterial activity against Aeromonas hydrophila, Bacillus brevis, B. cereus, Klebsiella pneumoniae, Pseudomonas aeruginosa, and Staphylococcus aureus ATCC 29213. However, no activity was found against Escherichia coli, Morganella morganii, Mycobacterium smegmatis, Proteus mirabilis, Yersinia enterocolitica, or Saccharomyces cerevisiae.

Anahtar Kelime: antimicrobial activity antioxidants phenolic compounds antimicrobial properties methanol Helichrysum medicinal plants plant extracts

Konular: Biyoloji

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	ALBAYRAK S, AKSOY A, SAGDIC O, Budak Ü (2010). Phenolic compounds and antioxidant and antimicrobial properties of Helichrysum species collected from eastern Anatolia, Turkey. , 463 - 473.
Chicago	ALBAYRAK SEVİL,AKSOY Ahmet,SAGDIC OSMAN,Budak Ümit Phenolic compounds and antioxidant and antimicrobial properties of Helichrysum species collected from eastern Anatolia, Turkey. (2010): 463 - 473.
MLA	ALBAYRAK SEVİL,AKSOY Ahmet,SAGDIC OSMAN,Budak Ümit Phenolic compounds and antioxidant and antimicrobial properties of Helichrysum species collected from eastern Anatolia, Turkey. , 2010, ss.463 - 473.
AMA	ALBAYRAK S,AKSOY A,SAGDIC O,Budak Ü Phenolic compounds and antioxidant and antimicrobial properties of Helichrysum species collected from eastern Anatolia, Turkey. . 2010; 463 - 473.
Vancouver	ALBAYRAK S,AKSOY A,SAGDIC O,Budak Ü Phenolic compounds and antioxidant and antimicrobial properties of Helichrysum species collected from eastern Anatolia, Turkey. . 2010; 463 - 473.
IEEE	ALBAYRAK S,AKSOY A,SAGDIC O,Budak Ü "Phenolic compounds and antioxidant and antimicrobial properties of Helichrysum species collected from eastern Anatolia, Turkey." , ss.463 - 473, 2010.
ISNAD	ALBAYRAK, SEVİL vd. "Phenolic compounds and antioxidant and antimicrobial properties of Helichrysum species collected from eastern Anatolia, Turkey". (2010), 463-473.

APA	ALBAYRAK S, AKSOY A, SAGDIC O, Budak Ü (2010). Phenolic compounds and antioxidant and antimicrobial properties of Helichrysum species collected from eastern Anatolia, Turkey. Turkish Journal of Biology, 34(4), 463 - 473.
Chicago	ALBAYRAK SEVİL,AKSOY Ahmet,SAGDIC OSMAN,Budak Ümit Phenolic compounds and antioxidant and antimicrobial properties of Helichrysum species collected from eastern Anatolia, Turkey. Turkish Journal of Biology 34, no.4 (2010): 463 - 473.
MLA	ALBAYRAK SEVİL,AKSOY Ahmet,SAGDIC OSMAN,Budak Ümit Phenolic compounds and antioxidant and antimicrobial properties of Helichrysum species collected from eastern Anatolia, Turkey. Turkish Journal of Biology, vol.34, no.4, 2010, ss.463 - 473.
AMA	ALBAYRAK S,AKSOY A,SAGDIC O,Budak Ü Phenolic compounds and antioxidant and antimicrobial properties of Helichrysum species collected from eastern Anatolia, Turkey. Turkish Journal of Biology. 2010; 34(4): 463 - 473.
Vancouver	ALBAYRAK S,AKSOY A,SAGDIC O,Budak Ü Phenolic compounds and antioxidant and antimicrobial properties of Helichrysum species collected from eastern Anatolia, Turkey. Turkish Journal of Biology. 2010; 34(4): 463 - 473.
IEEE	ALBAYRAK S,AKSOY A,SAGDIC O,Budak Ü "Phenolic compounds and antioxidant and antimicrobial properties of Helichrysum species collected from eastern Anatolia, Turkey." Turkish Journal of Biology, 34, ss.463 - 473, 2010.
ISNAD	ALBAYRAK, SEVİL vd. "Phenolic compounds and antioxidant and antimicrobial properties of Helichrysum species collected from eastern Anatolia, Turkey". Turkish Journal of Biology 34/4 (2010), 463-473.