Determination of ibuprofen in rabbit plasma by high-performance liquid chromatography
Yıl: 2010 Cilt: 16 Sayı: 6 Sayfa Aralığı: 1005 - 1010 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022
Determination of ibuprofen in rabbit plasma by high-performance liquid chromatography
Öz: Bu çalışmanın amacı tavşan plazmasında ibuprofenin tespit edilmesi için yüksek basınçlı sıvı kromatografisine (YBSK) dayalı bir yöntem geliştirmek ve valide etmektir. İbuprofen ve naproksenin (internal standart, IS) ayırımı dalga boyu λ=225 nm olan UV dedektör kullanılarak C18 kolon (5 μm, 250x4.6 mm i.d.) ile yapılmıştır. Mobil faz %0.1 trifluoroasetik asit içeren 20 mM fosfat tamponu (pH 7)-asetonitril (65:35, h/h) den oluşmuştur. Analiz 1 mL/dk akış hızı ile 10 dakikadan daha az sürede yapılmıştır. 0.5 ve 40 μg/mL arasında çok iyi linearite bulunmuştur. Plazmada ibuprofen için gün içi ve günler arası kesinlik değerleri %4.97’den küçüktü, ve doğruluk (bağıl hata) %7.20’den daha iyiydi. Bütün örnekler için geri kazanım >%92.8 idi. İbuprofenin saptanabilen (LOD) ve ölçülebilen (LOQ) en düşük değeri sırasıyla 0.10 and 0.25 μg/mL’dir. Tanımlanan YBSK metodu tavşanlarda ibuprofenin farmakokinetiğini incelemek için yeterli duyarlılığa ve özgüllüğe sahiptir ve klinik farmakokinetik çalışmalara da uyarlanabilir.
Anahtar Kelime: Konular:
Yüksek performanslı sıvı kromatografisi kullanarak tavşan plazmasında ibuprofenin tayini
Öz: The aim of the present study was to develop and validate a procedure based on high-performance liquid chromatography (HPLC) for determination of ibuprofen in rabbit plasma. Separation of ibuprofen and naproxen (internal standard, IS) was achieved on an Ace C18 column (5 μm, 250x4.6 mm i.d.) using UV detection with λ=225 nm. The mobile phase consisted of 20 mM phosphate buffer (pH 7) containing 0.1% trifluoroacetic acid (TFA)-acetonitrile (65:35, v/v). The analysis was performed in less than 10 min with a flow rate of 1 mL/min. Excellent linearity was found between 0.5 and 40 μg/mL. Intra- and inter-day precision values for ibuprofen in plasma were less than 4.97, and accuracy (relative error) was better than 7.20%. The recoveries for all samples were >92.8%. The limits of detection (LOD) and quantification (LOQ) of ibuprofen were 0.10 and 0.25 μg/mL, respectively. The described HPLC method has adequate sensitivity and specificity to study pharmacokinetics of ibuprofen in rabbits, and could be adapted also to clinical pharmacokinetic study.
Anahtar Kelime: Konular:
Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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| APA | Yılmaz B, AŞÇI A, PALABIYIK S (2010). Determination of ibuprofen in rabbit plasma by high-performance liquid chromatography. , 1005 - 1010. |
| Chicago | Yılmaz Bilal,AŞÇI Ali,PALABIYIK Saziye Sezin Determination of ibuprofen in rabbit plasma by high-performance liquid chromatography. (2010): 1005 - 1010. |
| MLA | Yılmaz Bilal,AŞÇI Ali,PALABIYIK Saziye Sezin Determination of ibuprofen in rabbit plasma by high-performance liquid chromatography. , 2010, ss.1005 - 1010. |
| AMA | Yılmaz B,AŞÇI A,PALABIYIK S Determination of ibuprofen in rabbit plasma by high-performance liquid chromatography. . 2010; 1005 - 1010. |
| Vancouver | Yılmaz B,AŞÇI A,PALABIYIK S Determination of ibuprofen in rabbit plasma by high-performance liquid chromatography. . 2010; 1005 - 1010. |
| IEEE | Yılmaz B,AŞÇI A,PALABIYIK S "Determination of ibuprofen in rabbit plasma by high-performance liquid chromatography." , ss.1005 - 1010, 2010. |
| ISNAD | Yılmaz, Bilal vd. "Determination of ibuprofen in rabbit plasma by high-performance liquid chromatography". (2010), 1005-1010. |
| APA | Yılmaz B, AŞÇI A, PALABIYIK S (2010). Determination of ibuprofen in rabbit plasma by high-performance liquid chromatography. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 16(6), 1005 - 1010. |
| Chicago | Yılmaz Bilal,AŞÇI Ali,PALABIYIK Saziye Sezin Determination of ibuprofen in rabbit plasma by high-performance liquid chromatography. Kafkas Üniversitesi Veteriner Fakültesi Dergisi 16, no.6 (2010): 1005 - 1010. |
| MLA | Yılmaz Bilal,AŞÇI Ali,PALABIYIK Saziye Sezin Determination of ibuprofen in rabbit plasma by high-performance liquid chromatography. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, vol.16, no.6, 2010, ss.1005 - 1010. |
| AMA | Yılmaz B,AŞÇI A,PALABIYIK S Determination of ibuprofen in rabbit plasma by high-performance liquid chromatography. Kafkas Üniversitesi Veteriner Fakültesi Dergisi. 2010; 16(6): 1005 - 1010. |
| Vancouver | Yılmaz B,AŞÇI A,PALABIYIK S Determination of ibuprofen in rabbit plasma by high-performance liquid chromatography. Kafkas Üniversitesi Veteriner Fakültesi Dergisi. 2010; 16(6): 1005 - 1010. |
| IEEE | Yılmaz B,AŞÇI A,PALABIYIK S "Determination of ibuprofen in rabbit plasma by high-performance liquid chromatography." Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 16, ss.1005 - 1010, 2010. |
| ISNAD | Yılmaz, Bilal vd. "Determination of ibuprofen in rabbit plasma by high-performance liquid chromatography". Kafkas Üniversitesi Veteriner Fakültesi Dergisi 16/6 (2010), 1005-1010. |
