A 41-year-old- female patient with neurobrucellosis is presented. The definite diagnosis was reached by the growth of the etiological agent in the cerebrospinal fluid (CSF). When adenosine deaminase (ADA) activity was compared with the normal levels, the activity was found to be three times higher in both serum and CSF (serum ADA=31.25 U/ml, CSF ADA=13.75 U/ml). In Computed Tomography (CT) and cranial Magnetic Resonance Imaging (MRI); cerebrocerebellar cortical atrophy, contrast enhancement in basal meninxes, tetraventricular minimal hydrocephalia and lesion showing high intensity in T2-weighted axial image in the left cerebral pedinculus were detected. Consequently, apart from hearing loss in the right ear, all clinical manifestations and laboratory abnormalities disappeared in the ; case following treatment with rifampin and doxycyclin used together. In the diagnosis of neurobrucellosis; the sophisticated radiological technology, such as CT and MRI, and some tests, such as ADA test in CSF, can be useful.