Determination of serum total sialic acid and ceruloplasmin concentrations in Toxoplasma gondii seropositive dogs

Yarim, Gul Fatma; ÖNCEL, Taraneh; HANDEMİR, EROL; NİSBET, Cevat; ÇENESİZ, Sena; ÇİFTCİ, GÜLAY

Determination of serum total sialic acid and ceruloplasmin concentrations in Toxoplasma gondii seropositive dogs

Cevat NİSBET, (Ondokuz Mayıs Üniversitesi, Veteriner Fakültesi, Samsun, Türkiye)

Sena ÇENESİZ, (Ondokuz Mayıs Üniversitesi, Veteriner Fakültesi, Samsun, Türkiye)

Taraneh ÖNCEL, (Pendik Veteriner Kontrol ve Araştırma, Enstitüsü, İstanbul, Türkiye)

Gul Fatma Yarim, (Ondokuz Mayıs Üniversitesi, Veteriner Fakültesi, Samsun, Türkiye)

Gülay ÇİFTCİ, (Ondokuz Mayıs Üniversitesi, Veteriner Fakültesi, Samsun, Türkiye)

Erol HANDEMİR (Veteriner Kontrol ve Araştırma Enstitüsü, Konya, Türkiye)

Etlik Veteriner Mikrobiyoloji Dergisi

2 0

Yıl: 2010 Cilt: 21 Sayı: 2 Sayfa Aralığı: 85 - 89 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022

Determination of serum total sialic acid and ceruloplasmin concentrations in Toxoplasma gondii seropositive dogs

Öz:

Bu çalışmada, İstanbul’un çeşitli barınaklarında bulunan ve Toxoplasma gondii seropozitif olan köpeklerin serum sialik asit (TSA) ve seruloplazmin (CP) düzeylerinin araştırılması ve bu düzeylerin arasında olası ilişkinin incelenmesi hedeflendi. Bu amaçla indirekt floresan antikor testi (IFAT) sonuçlarına göre T. gondii seronegatif bulunan 15 sağlıklı köpek ile seropozitif bulunan 25 enfekte köpekten kan örnekleri alındı ve serumları çıkarıldı. TSA ve CP değerleri ölçüldü. Yapılan analizlerde hasta ve kontrol gruba ait TSA düzeyleri sırası ile 17.20±0.55 mg/dl ve 15.97±0.55 mg/dl olarak tespit edildi. Buna göre hasta ve kontrol gruba ait serum TSA düzeyleri arasında istatistiksel olarak önemli bir fark bulunmadı (P>0.05). Serum SP ortalamaları hasta ve kontrol gruplarda sırasıyla 16.49±1.11 mg/dl ve 10.28±1.63 mg/dl olarak bulundu. Hasta grubun serum CP düzeyleri kontrol grubuyla karşılaştırıldığında anlamlı olarak yüksek bulundu (P<0.01). Diğer taraftan hasta ve kontrol grubuna ait serum TSA ile CP düzeyleri arasında herhangi bir korelasyon bulunamadı. Gruplar arası serum TSA düzeyinin anlamlı bulunmaması hastalığın kronik döneminde olduğunu ve hücresel hasarın ortadan kalktığını düşündürmektedir. T. gondii ile enfekte köpeklerde yüksek serum seruloplazmin düzeyinin, bu enfestasyon sonucunda oluşan hücre hasarının bir sonucu olduğu ve serum seruloplazmin düzeyinin bu hastalığın serolojik bulgularına yardımcı olarak kullanılabileceği kanaatine varılmıştır.

Anahtar Kelime: teşhis kan serumu Toxoplasma gondii köpek immünofloresan seruloplazmin hastalık belirleyiciler siyalik asit

Konular: Veterinerlik

Toksoplazma gondii yönünden seropozitif köpeklerin serum total sialik asit ve seruloplazmin düzeylerinin belirlenmesi

Öz:

This study aimed to investigate serum total sialic acid (TSA) and ceruloplasmin (CP) levels and their possible correlation in Toxoplasma gondii seropositive dogs. The study was conducted on the dogs that had been kept in doghouses located in the province of Istanbul. Based upon indirect fluorescence antibody test (IFAT) results, 15 T. gondii seronegative dogs and 25 seropositive infected dogs were included in the study. Blood samples were collected from each dog and serums were obtained. Subsequently, TSA and CP concentrations were measured. For serum TSA concentration, no significant difference was found between control (17.20±0.55 mg/dl) and infected dogs (15.97±0.55 mg/dl) (p>0.05). On the other hand, serum CP concentration was significantly higher in seropositive dogs (16.49±1.11 mg/dl) compared to control dogs (10.28 ±1.63 mg/dl) (p<0.01). No correlation was found between serum TSA and serum CP. Absence of a significant difference between groups for serum TSA might be related to the fact that the disease was in chronic stage, and the severity of cellular damage might have been decreased. Elevation of serum CP in T. gondii infected dogs is most likely related to cellular damage as a consequence of infection. We suggest that serum CP can be used as an additional prognostic parameter in toxoplasmosis in support to other serologic parameters.

Anahtar Kelime: dogs immunofluorescence ceruloplasmin disease markers sialic acids diagnosis blood serum Toxoplasma gondii

Konular: Veterinerlik

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

1. Canon-Franco WA, Bergamaschi DP, Labruna MB, Camargo lM, Silva JC, Pinter A, Gennari SM, (2004). Occurrence of anti-Toxoplasma gondii antibodies in dogs in the urban area of Monte Negro, Rondonia, Brazil. Vet Res Com. 28, 113- 118.
2. Cetin M, Gunes N, Aydin L, (1995). Gasterointestinal nematodlarla enfekte köpeklerin plazma vitamin C, seruloplazmin ve total protein düzeylerindeki değişimler. Veterinarium. 6, 76-78.
3. Chen CL, Tang FT, Chen HC, Chung CY, Wong MK, (2000). Brain lesion size and location: effects on motor recovery and functional outcome in stroke patients. Arch Phys Med Rehabil. 81, 447-452.
4. Colombo JP, Richterich R, (1964). Zur Bestimmung Des Caeruloplasmin im plasma. Schweiz Med Wochen. 94, 715-720.
5. Cooper ES, Ramdath DD, Whyte-alleng C, Howell S, Serjeant BE, (1997). Plasma proteins in children with trichuris dysentery syndrome. J Clin Pathol. 50, 236–240.
6. Eckersall PD, Hall FR, Brown CGD, (2003). The protozoan parasite, Theileria annulata, induces a distinct acute phase protein response in cattle that is associated with pathology. Int J Parasitol. 33, 1409-1418.
7. Eckersall PD, Young FJ, Mccomb C, Hogarth CJ, Safi S, Weber A, Mcdonald T, Nolan AM, Fitzpatrick JL, (2001). Acute phase proteins in serum and milk from dairy cows with clinical mastitis. Vet Rec. 148, 35-41.
8. Floris G, Medda R, Padiglia A, Musci G, (2000). The physiopathological significance of ceruloplasmin A possible therapeutic approach. Biochemical Pharmacol. 60, 1735-1741.
9. Foudnier F, Chemia CM, Pinon J, (1995). Value of specific immunglobulin A detection by two immunocapture assays in the diagnosis of toxoplasmosis. Eur J Clin Microbiol Infect Dis. 14, 585-590.
10. Glass EJ, Craigmile SC, Springbett A, Preston PM, Kirvar E, Wilkie GM, Eckersall PD, Hall FR, Brown CG, (2003). The protozoan parasite, Theileria annulata, induces a distinct acute phase protein response in cattle that is associated with pathology. Int J Parasitol. 12, 1409-1418.
11. Glurich I, Grossi S, Albini B, Ho A, Shah R, Zeid M, Baumann H, Robert J, Genco RJ, Nardin FD, (2002). Systemic inflammation in cardiovascular and periodontal disease: comparative Study. Clin Diagn Lab Immunol. 9, 425-432.
12. Gungor O, Sunar B, Ozcelik F, Aktas Z, Gokmen SS, (2004). Serum sialic acid levels in acute myocardial infar ction and relationship to ceruloplasmin. Turk J Biochem. 29, 226- 231.
13. Halliwell B, Gutteridge JM, (1990). The antioxidants of human extracellular fluids. Arch Biochem Biophys. 280, 1-8.
14. Hsu CC, Lin TW, Chang WW, Wu CY, Lo WH, Wang PH, Tsai YC, (2005). Soyasaponin-I-modified invasive behavior of cancer by changing cell surface sialic acids. Gynecol Oncol. 96, 415-422.
15. Kaufmann J, (1996). Parasitic Infections of Domestic Animals. A Diagnostic Manual. Birkhauser Verlag. Basel- Boston-Berlin.
16. Keles I, Ertekin A, Karaca M, Erkin S, Akkan HA, (2000). Sığırların leptospirozisinde serum sialik asit ve lipid-bağlı sialik asit düzeyleri üzerine araştıma. Yuzuncu Yıl Üniv Vet Fak Derg, 11, 121-122.
17. Kurdova R, Krüger D, Janitschke K, (1998). Polymerase chain reaction (PCR) in the laboratory diagnosis of acute toxoplasmosis. Exp Pathol Parasitol. 1, 55-61.
18. Ponnio M, Alho H, Nikkari ST, Olsson U, Rydberg U, Sillanaukee P, (1999). Serum sialic acid in a random sample of the general population. Clin Chem. 45, 1842-1849.
19. Ronday MJH, Ongkosuwito JV, Rothova A, Kijlstra A, (1999). Intraocular Anti- Toxoplasma gondii IgA Antibody Production in Patients With Ocular Toxoplasmosis. Amer J Ophthalmol. 127, 294-300.
20. Silva DAO, Cabral DD, Bernardina BLD, Souza MA, Mineo JR, (1997). Detection of Toxoplasma gondii specific antibodies in dogs. A comparative study of immunoenzymatic, immunoflurescent and haemagglutination titres. Memórias do Instituto Oswaldo Cruz. 92, 785-789.
21. Tanaka T, Abe Y, Inoue N, Kim WS, Kumura H, Nagasawa H, Igarashi I, Shimazaki K, (2004). The detection of bovine lactoferrin binding protein on Trypanosoma brucei. J Vet Med Sci. 66, 619-625.
22. Tenter AM, Heckeroth AR, Weiss lM, (2000). Toxoplasma gondii from animal to humans. Int J Parasitol. 30, 1217-1258.
23. Tuzer E, Toparlak M, (1999). Veterinary Parasitology Lecture Notes. Istanbul University, Faculty of Veterinary Medicine:105, Istanbul.
24. Verazin G, Riley WM, Gregory J, Tautu C, Prorok JJ, Alhadeff JA, (1990). Serum sialic acid and carcinoembryonic levels in the detection and monitoring of colorectal cancer. Dis Colon & Rectum. 33, 139-142.
25. Wang P, Zhang J, Bian H, Wu P, Kuvelkar R, Kung TT, Crawley Y, Egan RW, Billah MM, (2004). Induction of lysosomal and plasma membrane-bound sialidases in human T-cells via T-cell receptor. Biochem J. 380, 425-433.
26. Warren L, (1959). The thiobarbituric acid assay of sialic acids. J Biol Chem. 234, 1971-1975.
27. Wilson JC, Kiefel MJ, Albouz-abo S, Von-itzstein M, (2000). Preliminary 1H NMR investigation of sialic acid transfer by the trans-sialidase from Trypanosoma cruzi. Bioorg Med Chem Lett. 10, 2791-2794.

APA	NİSBET C, ÇENESİZ S, ÖNCEL T, Yarim G, ÇİFTCİ G, HANDEMİR E (2010). Determination of serum total sialic acid and ceruloplasmin concentrations in Toxoplasma gondii seropositive dogs. , 85 - 89.
Chicago	NİSBET Cevat,ÇENESİZ Sena,ÖNCEL Taraneh,Yarim Gul Fatma,ÇİFTCİ GÜLAY,HANDEMİR EROL Determination of serum total sialic acid and ceruloplasmin concentrations in Toxoplasma gondii seropositive dogs. (2010): 85 - 89.
MLA	NİSBET Cevat,ÇENESİZ Sena,ÖNCEL Taraneh,Yarim Gul Fatma,ÇİFTCİ GÜLAY,HANDEMİR EROL Determination of serum total sialic acid and ceruloplasmin concentrations in Toxoplasma gondii seropositive dogs. , 2010, ss.85 - 89.
AMA	NİSBET C,ÇENESİZ S,ÖNCEL T,Yarim G,ÇİFTCİ G,HANDEMİR E Determination of serum total sialic acid and ceruloplasmin concentrations in Toxoplasma gondii seropositive dogs. . 2010; 85 - 89.
Vancouver	NİSBET C,ÇENESİZ S,ÖNCEL T,Yarim G,ÇİFTCİ G,HANDEMİR E Determination of serum total sialic acid and ceruloplasmin concentrations in Toxoplasma gondii seropositive dogs. . 2010; 85 - 89.
IEEE	NİSBET C,ÇENESİZ S,ÖNCEL T,Yarim G,ÇİFTCİ G,HANDEMİR E "Determination of serum total sialic acid and ceruloplasmin concentrations in Toxoplasma gondii seropositive dogs." , ss.85 - 89, 2010.
ISNAD	NİSBET, Cevat vd. "Determination of serum total sialic acid and ceruloplasmin concentrations in Toxoplasma gondii seropositive dogs". (2010), 85-89.

APA	NİSBET C, ÇENESİZ S, ÖNCEL T, Yarim G, ÇİFTCİ G, HANDEMİR E (2010). Determination of serum total sialic acid and ceruloplasmin concentrations in Toxoplasma gondii seropositive dogs. Etlik Veteriner Mikrobiyoloji Dergisi, 21(2), 85 - 89.
Chicago	NİSBET Cevat,ÇENESİZ Sena,ÖNCEL Taraneh,Yarim Gul Fatma,ÇİFTCİ GÜLAY,HANDEMİR EROL Determination of serum total sialic acid and ceruloplasmin concentrations in Toxoplasma gondii seropositive dogs. Etlik Veteriner Mikrobiyoloji Dergisi 21, no.2 (2010): 85 - 89.
MLA	NİSBET Cevat,ÇENESİZ Sena,ÖNCEL Taraneh,Yarim Gul Fatma,ÇİFTCİ GÜLAY,HANDEMİR EROL Determination of serum total sialic acid and ceruloplasmin concentrations in Toxoplasma gondii seropositive dogs. Etlik Veteriner Mikrobiyoloji Dergisi, vol.21, no.2, 2010, ss.85 - 89.
AMA	NİSBET C,ÇENESİZ S,ÖNCEL T,Yarim G,ÇİFTCİ G,HANDEMİR E Determination of serum total sialic acid and ceruloplasmin concentrations in Toxoplasma gondii seropositive dogs. Etlik Veteriner Mikrobiyoloji Dergisi. 2010; 21(2): 85 - 89.
Vancouver	NİSBET C,ÇENESİZ S,ÖNCEL T,Yarim G,ÇİFTCİ G,HANDEMİR E Determination of serum total sialic acid and ceruloplasmin concentrations in Toxoplasma gondii seropositive dogs. Etlik Veteriner Mikrobiyoloji Dergisi. 2010; 21(2): 85 - 89.
IEEE	NİSBET C,ÇENESİZ S,ÖNCEL T,Yarim G,ÇİFTCİ G,HANDEMİR E "Determination of serum total sialic acid and ceruloplasmin concentrations in Toxoplasma gondii seropositive dogs." Etlik Veteriner Mikrobiyoloji Dergisi, 21, ss.85 - 89, 2010.
ISNAD	NİSBET, Cevat vd. "Determination of serum total sialic acid and ceruloplasmin concentrations in Toxoplasma gondii seropositive dogs". Etlik Veteriner Mikrobiyoloji Dergisi 21/2 (2010), 85-89.