TY  - JOUR
TI  - Influences of basal media, growth regulators, explant type and  photoperiod on callus competency and pigmentation of Alkanna  orientalis L.
AB  - The goal of this research was in-vitro callus induction and red pigment production of Alkanna  orientalis as a medicinal herb which belongs to Boraginaceae family containing valuable  naphthoquinone derivates. The two different explants (leaf and leaf base) were subjected to  abiotic factors such as different nutrient media (MS, B5 and M9), light and plant growth  regulators (IAA and IBA). High frequency reproducible, prolific and compact calli formation  was obtained from MS and B5 media supplemented with IAA, whereas high pigmentation was  found in leaf base explants on M9 medium. Leaf base explant and dark conditions were found  more effective for both callus formation and pigment production. The pigmentation at IBA  was more than IAA, and the maximum level of pigmentation was observed on M9 medium  with a combination of 1.0 mg/l IBA under dark. The factors that may be the most influential  in the production of callus and red color pigment from A. orientalis have been determined. A.  orientalis may be considered to be alternative plants for the A/S (alkannin/shikonin)  production in vitro.
AU  - URANBEY, Serkan
AU  - YAMAN, Cennet
AU  - Bakhsh, Allah
DO  - 10.17776/csj.933232
PY  - 2021
JO  - Cumhuriyet Science Journal
VL  - 42
IS  - 4
SN  - 2587-2680
SP  - 766
EP  - 774
DB  - TRDizin
UR  - http://search/yayin/detay/1110441
ER  -