TY  - JOUR
TI  - Recombinant Production of E. coli NAD+-dependent DNA ligase as a Target for Antibacterial  Drug Discovery
AB  - The increase in the frequency of drug resistance in bacterial infections  has led to the research of new antibacterial agents targeting new  mechanisms. Many of the functions of NAD+-dependent DNA ligase  have made it a remarkable target for antibacterial drug discovery.  Escherichia coli (E. coli) NAD+-dependent DNA ligase is presented as  a potential target due to its unique substrate specificity compared to  the ATP-dependent human DNA ligase. In this study, it was aimed to  produce and purify the E. coli NAD + dependent DNA ligase enzyme,  which is frequently used in antibacterial drug discovery. The E. coli DNA ligase gene sequence was cloned into pTOLT vector system. E.  coli DNA ligase enzyme was purified after the production in E. coli  BL21 (DE3) pLysE cells. It was clearly demonstrated by the activity  test that the DNA ligase enzyme produced in this study can ligate the  DNA fragments. As a result, it was revealed that the effect of  candidate inhibitors can be studied simply on the enzyme.
AU  - IMAMOGLU, RIZVAN
AU  - GOKCE, Isa
AU  - Kaplan, Ozlem
DO  - 10.18016/ksutarimdoga.vi.884279
PY  - 2022
JO  - Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi
VL  - 25
IS  - 1
SN  - 2619-9149
SP  - 19
EP  - 24
DB  - TRDizin
UR  - http://search/yayin/detay/1116953
ER  -