Introduction: The study was conducted to determine the pre-vaccination anxiety levels of parents with children between the ages of 0-2. Method: The cross-sectional study was conducted between May and September 2020 at the one Family Health Center (FHC) in Adıyaman. All parents with children between 0-2 years old who were registered with FHC at the time of the study (n=488). Personal Information Form, State Anxiety Scale (SAI) and Trait Anxiety Inventory (TAI) were used to collect data. Result: Parents' mean SAI score is 36.90±8.99 for women and 36.50±10.61 for men (p>0.05); The mean score of TAI was found to be 44.93±8.45 for women and 38.74±8.26 for men (p<0.05). In the study, it was determined that the average SAI scores of parents aged 30 and over (p=0.004), high income level (p=0.010) and those who were indecisive about vaccination (p<0.001) were higher. In addition, among the parents, those who were illiterate (p<0.001), those with low income (p<0.001), those whose children were not healthy (p=0.008), those who had their children vaccinated at the 18th month (p=0.020), those who were indecisive about vaccination (p=0.030) and those who did not know about the vaccine (p=0.026) were found to have higher mean TAI scores. Conclusion: The results of the study showed that the state anxiety levels of the mothers and fathers were similar before vaccination, and the trait anxiety level experienced by the mothers was higher than the fathers. In addition, it was determined that the state and trait anxiety of the parents were affected by some variables.