Objective: The aim of this study is to determine the level of compliance of pediatric nurses to isolation measures and the affecting factors during the Covid-19 process.Materials and Methods: The population of the study consisted of 96 pediatric nurses working in a university hospital in Erzurum. The sampling method was not used and the research was completed with 87 pediatric nurses. Data collection tools were collected by face-to-face interview method with the “Question Form” and “Scale of Adaptation to Isolation Precautions (PSPS)”.Results: In the study, 72.5% of the pediatric nurses did not attend the isolation precautions training during the Covid-19 process, 95.4% applied the isolation procedures to their patients, 82.8% applied the contact procedure. It was determined that 87.4% of them were able to allocate a room for isolation and 83.9% of them had no problems in applying the isolation measures. The total score obtained by the nurses in the BSQ was found to be 74.78±6.71.Conclusion: It was determined that the level of compliance of the nurses with the isolation measures was good. It was found that the level of compliance with isolation measures was higher in the nurses who were married and had more professional experience.