Yıl: 2022 Cilt: 46 Sayı: 2 Sayfa Aralığı: 350 - 363 Metin Dili: İngilizce DOI: 10.33483/jfpau.1073081 İndeks Tarihi: 07-09-2022

PREPARATION AND IN VITRO CHARACTERIZATION OF VANCOMYCIN LOADED PLGA NANOPARTICLES FOR THE TREATMENT OF ENTEROCOCCUS FAECALIS INFECTIONS

Öz:
Objective: In this study, it was aimed to prepare Vancomycin loaded poly(lactic acid-co-glycolic acid) (PLGA) nanoparticles that can be used parenterally in enterococcal infections. Material and Method: In the study, different concentrations of PLGA were used to prepare nanoparticles. Emulsification-solvent evaporation method was used in the preparation of the particles. Particle size and distribution, zeta potential, encapsulation efficiency and yield of the prepared formulations were determined. FTIR analyses were also performed for the formulations. The antibacterial activity was determined as a minimum inhibition concentration by broth microdilution method and as an inhibition zone by disk diffusion method against Enterococcus faecalis ATCC 29212 according to European Committee on Antimicrobial Susceptibility Testing and its effectiveness on bacteria was interpreted by comparing with the pure active substance. Result and Discussion: Particles were successfully prepared by emulsion-solvent evaporation method. Around 300 nm; Particles with a high amount of encapsulated Vancomycin were obtained at PDI values of 0.26-0.28. As a result of the FTIR analysis, it was determined that Vancomycin was loaded into the particle. The MIC values of the prepared PLGA nanoparticles against Enterococcus faecalis ATCC 29212 strain was found to be 4 μg/ml, and the inhibition zone diameters were measured as 15 mm, 15 mm and 16 mm, respectively.
Anahtar Kelime:

ENTEROKOK ENFEKSİYONLARINDA KULLANILMAK ÜZERE VANCOMİSİN İÇEREN PLGA NANOPARTİKÜLLERİN HAZIRLANMASI VE KARAKTERİZASYONU

Öz:
Amaç: Bu çalışmada enterokok enfeksiyonlarında parenteral olarak kullanılabilecek Vankomisin yüklü poli(laktik asit-ko-glikolik asit) (PLGA) nanopartikülleri hazırlanması amaçlanmıştır. Gereç ve Yöntem: Çalışmada, nanopartikülleri hazırlamak amacıyla farklı konsantrasyonlarda PLGA kullanılmıştır. Partiküllerin hazırlanmasında emülsiyon oluşturma-çözücü buharlaştırma yöntemi kullanılmıştır. Hazırlanan formülasyonların partikül büyüklüğü ve dağılımı, zeta potansiyeli, enkapsülasyon etkinliği, üretim verimi ölçülmüştür ve FTIR analizi yapılmıştır. Enterococcus faecalis ATCC 29212 suşu üzerine antibakteriyel aktivite sıvı mikrodilüsyon yöntemi ile minimum inhibisyon konsantrasyonu olarak ve disk difüzyon yöntemi ile inhibisyon zonu olarak Avrupa Antimikrobiyal Duyarlılık Testi Komitesi standartlarına göre belirlenmiş, sonuçlar saf etken madde ile karşılaştırılarak yorumlanmıştır. Sonuç ve Tartışma: Partiküller emülsiyon-çözücü buharlaştırma yöntemi ile başarılı bir şekilde hazırlanmıştır. Yaklaşık 300 nm civarında; 0.26-0.28 PDI değerlerinde, enkapsüle olan Vankomisin miktarı oldukça yüksek partiküller elde edilmiştir. FTIR ve DSC analizi sonucu vankomisinin partikül içine yüklendiği tespit edilmiştir. Hazırlanan PLGA nanopartiküllerin Enterococcus faecalis ATCC 29212 suşu üzerine MİK değerleri 4 μg/ml bulunmuş, inhibisyon zon çapları ise sırasıyla 15 mm, 15 mm ve 16 mm olarak ölçülmüştür.
Anahtar Kelime:

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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APA TOPAL G, Kiymaci M, OZKAN Y (2022). PREPARATION AND IN VITRO CHARACTERIZATION OF VANCOMYCIN LOADED PLGA NANOPARTICLES FOR THE TREATMENT OF ENTEROCOCCUS FAECALIS INFECTIONS. , 350 - 363. 10.33483/jfpau.1073081
Chicago TOPAL Gizem Ruya,Kiymaci Merve Eylul,OZKAN YALCIN PREPARATION AND IN VITRO CHARACTERIZATION OF VANCOMYCIN LOADED PLGA NANOPARTICLES FOR THE TREATMENT OF ENTEROCOCCUS FAECALIS INFECTIONS. (2022): 350 - 363. 10.33483/jfpau.1073081
MLA TOPAL Gizem Ruya,Kiymaci Merve Eylul,OZKAN YALCIN PREPARATION AND IN VITRO CHARACTERIZATION OF VANCOMYCIN LOADED PLGA NANOPARTICLES FOR THE TREATMENT OF ENTEROCOCCUS FAECALIS INFECTIONS. , 2022, ss.350 - 363. 10.33483/jfpau.1073081
AMA TOPAL G,Kiymaci M,OZKAN Y PREPARATION AND IN VITRO CHARACTERIZATION OF VANCOMYCIN LOADED PLGA NANOPARTICLES FOR THE TREATMENT OF ENTEROCOCCUS FAECALIS INFECTIONS. . 2022; 350 - 363. 10.33483/jfpau.1073081
Vancouver TOPAL G,Kiymaci M,OZKAN Y PREPARATION AND IN VITRO CHARACTERIZATION OF VANCOMYCIN LOADED PLGA NANOPARTICLES FOR THE TREATMENT OF ENTEROCOCCUS FAECALIS INFECTIONS. . 2022; 350 - 363. 10.33483/jfpau.1073081
IEEE TOPAL G,Kiymaci M,OZKAN Y "PREPARATION AND IN VITRO CHARACTERIZATION OF VANCOMYCIN LOADED PLGA NANOPARTICLES FOR THE TREATMENT OF ENTEROCOCCUS FAECALIS INFECTIONS." , ss.350 - 363, 2022. 10.33483/jfpau.1073081
ISNAD TOPAL, Gizem Ruya vd. "PREPARATION AND IN VITRO CHARACTERIZATION OF VANCOMYCIN LOADED PLGA NANOPARTICLES FOR THE TREATMENT OF ENTEROCOCCUS FAECALIS INFECTIONS". (2022), 350-363. https://doi.org/10.33483/jfpau.1073081
APA TOPAL G, Kiymaci M, OZKAN Y (2022). PREPARATION AND IN VITRO CHARACTERIZATION OF VANCOMYCIN LOADED PLGA NANOPARTICLES FOR THE TREATMENT OF ENTEROCOCCUS FAECALIS INFECTIONS. Ankara Üniversitesi Eczacılık Fakültesi Dergisi, 46(2), 350 - 363. 10.33483/jfpau.1073081
Chicago TOPAL Gizem Ruya,Kiymaci Merve Eylul,OZKAN YALCIN PREPARATION AND IN VITRO CHARACTERIZATION OF VANCOMYCIN LOADED PLGA NANOPARTICLES FOR THE TREATMENT OF ENTEROCOCCUS FAECALIS INFECTIONS. Ankara Üniversitesi Eczacılık Fakültesi Dergisi 46, no.2 (2022): 350 - 363. 10.33483/jfpau.1073081
MLA TOPAL Gizem Ruya,Kiymaci Merve Eylul,OZKAN YALCIN PREPARATION AND IN VITRO CHARACTERIZATION OF VANCOMYCIN LOADED PLGA NANOPARTICLES FOR THE TREATMENT OF ENTEROCOCCUS FAECALIS INFECTIONS. Ankara Üniversitesi Eczacılık Fakültesi Dergisi, vol.46, no.2, 2022, ss.350 - 363. 10.33483/jfpau.1073081
AMA TOPAL G,Kiymaci M,OZKAN Y PREPARATION AND IN VITRO CHARACTERIZATION OF VANCOMYCIN LOADED PLGA NANOPARTICLES FOR THE TREATMENT OF ENTEROCOCCUS FAECALIS INFECTIONS. Ankara Üniversitesi Eczacılık Fakültesi Dergisi. 2022; 46(2): 350 - 363. 10.33483/jfpau.1073081
Vancouver TOPAL G,Kiymaci M,OZKAN Y PREPARATION AND IN VITRO CHARACTERIZATION OF VANCOMYCIN LOADED PLGA NANOPARTICLES FOR THE TREATMENT OF ENTEROCOCCUS FAECALIS INFECTIONS. Ankara Üniversitesi Eczacılık Fakültesi Dergisi. 2022; 46(2): 350 - 363. 10.33483/jfpau.1073081
IEEE TOPAL G,Kiymaci M,OZKAN Y "PREPARATION AND IN VITRO CHARACTERIZATION OF VANCOMYCIN LOADED PLGA NANOPARTICLES FOR THE TREATMENT OF ENTEROCOCCUS FAECALIS INFECTIONS." Ankara Üniversitesi Eczacılık Fakültesi Dergisi, 46, ss.350 - 363, 2022. 10.33483/jfpau.1073081
ISNAD TOPAL, Gizem Ruya vd. "PREPARATION AND IN VITRO CHARACTERIZATION OF VANCOMYCIN LOADED PLGA NANOPARTICLES FOR THE TREATMENT OF ENTEROCOCCUS FAECALIS INFECTIONS". Ankara Üniversitesi Eczacılık Fakültesi Dergisi 46/2 (2022), 350-363. https://doi.org/10.33483/jfpau.1073081