TY  - JOUR
TI  - The importance of FISH Test Targeting EGFR, CCND1 and RREB1 Genes in Differentiating Malignant Melanomas from Melanocytic Nevus
AB  - Melanocytic nevus (MN) may on occasion be difficult to distinguish from malignant melanoma (MM) histopathologically. Fluorescent in situ hybridization (FISH) has been demonstrated to be of use for the diagnosis of melanocytic neoplasms of the skin. In this study, the effectiveness of the standart melanoma FISH test (4-way probe targeting RREB1, CCND1, MYB genes and centromere 6) and additionally probes, targetting EGFR, TP53, MDM2 and TP53 genes,  in differentiating melanomas from melanocytic nevi were investigated. Standard FISH test was performed on 24 MM and 24 MN samples, but  EGFR, TP53, MDM2 and TP53 gene copy numbers were investigated in 16 of 24 MM and 24 MN using FISH method. The incidence of FISH-detected positive genomic copy aberrations (4-way probe, and others) was determined as 83,3% in 24 MM cases, and 5,2% in 24 MN. Statistically significant differences were found between the MM and MN groups in terms of CCND1, RREB1, EGFR amplifications (p<0.001, p<0.05, p˂0.05), but there was no association between histopathological features and detected abnormalities (p>0,05). In additionally, all 5 acral lentiginous melanomas, could be analysed, had EGFR amplifications. In conclusion, CCND1, RREB1, and EGFR amplifications have diagnostic significance for MM. The FISH test is very effective in terms of its use as an adjunct to histopathological methods. But centromere controlled probes should be used to avoid false positive results.
AU  - Ozen, Hulya
AU  - Artan, Sevilhan
AU  - durak aras, beyhan
AU  - TÖRE, Tolga
AU  - IŞIK, SEVGİ
AU  - Canaz, Funda
AU  - Erzurumluoglu Gokalp, Ebru
AU  - ÇILINGIR, OGUZ
DO  - 10.20515/otd.886981
PY  - 2021
JO  - Osmangazi Tıp Dergisi
VL  - 43
IS  - 5
SN  - 1305-4953
SP  - 431
EP  - 438
DB  - TRDizin
UR  - http://search/yayin/detay/1119004
ER  -