Kronik Lenfositik Lösemide Ölçülebilir Kalıntı Hastalık: Kuru Tüp Akım Sitometri Metoduyla Gerçek Yaşam Deneyimi
Yıl: 2022 Cilt: 10 Sayı: 2 Sayfa Aralığı: 147 - 154 Metin Dili: Türkçe DOI: 10.4274/nkmj.galenos.2022.93063 İndeks Tarihi: 12-09-2022
Kronik Lenfositik Lösemide Ölçülebilir Kalıntı Hastalık: Kuru Tüp Akım Sitometri Metoduyla Gerçek Yaşam Deneyimi
Öz: Amaç: Kemoimmünoterapi (Kİ) sonrası ölçülebilir kalıntı hastalığın (ÖKH) negatifleşmesi uzun dönem progresyonsuz sağkalım (PS) ve genel sağkalım ile ilişkilidir. Ancak klinik çalışmalar dışında tedavi edilen hastalarda ÖKH negatifliğinin sağkalım üzerine etkisi belirsizdir. Kuru antikor tüp metodu gibi pipet kullanılmayan antikor boyama yöntemleri işlem ilişkili hataları azaltabilir ve daha iyi standardizasyon sağlayabilir. Fakat bu yöntemin kullanıldığı klinik veri bulunmamaktadır. Çalışmamızda kronik lenfositik lösemi (KLL) olgularının tedavisinde kuru antikor tüp metodunun etkililiğinin belirlenmesi amaçlandı. Gereç ve Yöntem: Kİ uygulanan ve tedavinin bitiminden sonraki 6 ayda ÖKH analizi yapılan KLL hastalarının verileri geriye dönük olarak analiz edildi. Çalışmaya 46 hasta dahil edildi. ÖKH, çoğunlukla kuru tüp metodu kullanılarak, hassasiyeti 10-4 olan akım sitometri ile değerlendirildi. Bulgular: Otuz (%65,2) hastada ÖKH negatifliği sağlandı. ÖKH negatifleşenlerde medyan PS süresi negatifleşmeyenlere kıyasla daha uzundu. Çalışma sürecinde 29 hasta yalnızca kuru tüp metodu ile değerlendirildi. Kuru tüp metodu ile çalışılan hastalar ayrıca değerlendirildiğinde ÖKH negatifliğinin uzamış PS ile ilişkili olduğu görüldü. Sonuç: Çalışmamız KLL hastalarında akım sitometri temelli ÖKH izleminin klinik çalışmalardakine benzer şekilde PS açısından prognostik önemini ortaya koydu. Ayrıca kuru antikor paneli (DuraClone RE CLB Tube) yöntemi klinik pratikte ilk kez kullanıldı.
Anahtar Kelime: Measurable Residual Disease in Chronic Lymphocytic Leukemia: Experience in Real-Life Setting with Dry Tube Flow Cytometric Method
Öz: Aim: Undetectable measurable residual disease (uMRD) after chemoimmunotherapy (CI) is associated longer progression-free survival (PFS) and overall survival. However, it remains to be demonstrated whether uMRD translates into survival benefit in patients treated outside of clinical trials. Pipetting-free antibody staining procedures such as dry antibody tube method can reduce process-related errors and provide a better standardization. However, there are no clinical data about this method. The aim of the study was to evaluate the impact of dry antibody tube-based MRD analysis in the management of chronic lymphocytic leukemia (CLL). Materials and Methods: We retrospectively reviewed the data of CLL patients, who were treated with CI regimens and had MRD analysis within 6 months after therapy. Forty-six patients were included in the study. MRD was assessed by multi-color flow cytometry panels with a sensitivity level of 10-4, mostly with dry tube. Results: uMRD was achieved in 30 (65.2%) of the patients. The median PFS of patients who achieved uMRD was significantly longer compared to patients who did not. Twenty-nine patients were analyzed only by dry tube throughout study period. In the patients studied with the dry tube method, the median PFS of the ones who achieved uMRD was also significantly longer than those who did not. Conclusion: Our study has indicated that flow cytometry based MRD surveillance of CLL patients in real-life setting provides prognostic information regarding PFS in accordance with clinical studies. In addition, clinical data of dry antibody panel (DuraClone RE CLB Tube) were presented for the first time.
Anahtar Kelime: Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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| APA | AKPINAR S, Turgut B (2022). Kronik Lenfositik Lösemide Ölçülebilir Kalıntı Hastalık: Kuru Tüp Akım Sitometri Metoduyla Gerçek Yaşam Deneyimi. , 147 - 154. 10.4274/nkmj.galenos.2022.93063 |
| Chicago | AKPINAR SEVAL,Turgut Burhan Kronik Lenfositik Lösemide Ölçülebilir Kalıntı Hastalık: Kuru Tüp Akım Sitometri Metoduyla Gerçek Yaşam Deneyimi. (2022): 147 - 154. 10.4274/nkmj.galenos.2022.93063 |
| MLA | AKPINAR SEVAL,Turgut Burhan Kronik Lenfositik Lösemide Ölçülebilir Kalıntı Hastalık: Kuru Tüp Akım Sitometri Metoduyla Gerçek Yaşam Deneyimi. , 2022, ss.147 - 154. 10.4274/nkmj.galenos.2022.93063 |
| AMA | AKPINAR S,Turgut B Kronik Lenfositik Lösemide Ölçülebilir Kalıntı Hastalık: Kuru Tüp Akım Sitometri Metoduyla Gerçek Yaşam Deneyimi. . 2022; 147 - 154. 10.4274/nkmj.galenos.2022.93063 |
| Vancouver | AKPINAR S,Turgut B Kronik Lenfositik Lösemide Ölçülebilir Kalıntı Hastalık: Kuru Tüp Akım Sitometri Metoduyla Gerçek Yaşam Deneyimi. . 2022; 147 - 154. 10.4274/nkmj.galenos.2022.93063 |
| IEEE | AKPINAR S,Turgut B "Kronik Lenfositik Lösemide Ölçülebilir Kalıntı Hastalık: Kuru Tüp Akım Sitometri Metoduyla Gerçek Yaşam Deneyimi." , ss.147 - 154, 2022. 10.4274/nkmj.galenos.2022.93063 |
| ISNAD | AKPINAR, SEVAL - Turgut, Burhan. "Kronik Lenfositik Lösemide Ölçülebilir Kalıntı Hastalık: Kuru Tüp Akım Sitometri Metoduyla Gerçek Yaşam Deneyimi". (2022), 147-154. https://doi.org/10.4274/nkmj.galenos.2022.93063 |
| APA | AKPINAR S, Turgut B (2022). Kronik Lenfositik Lösemide Ölçülebilir Kalıntı Hastalık: Kuru Tüp Akım Sitometri Metoduyla Gerçek Yaşam Deneyimi. Namık Kemal Tıp Dergisi, 10(2), 147 - 154. 10.4274/nkmj.galenos.2022.93063 |
| Chicago | AKPINAR SEVAL,Turgut Burhan Kronik Lenfositik Lösemide Ölçülebilir Kalıntı Hastalık: Kuru Tüp Akım Sitometri Metoduyla Gerçek Yaşam Deneyimi. Namık Kemal Tıp Dergisi 10, no.2 (2022): 147 - 154. 10.4274/nkmj.galenos.2022.93063 |
| MLA | AKPINAR SEVAL,Turgut Burhan Kronik Lenfositik Lösemide Ölçülebilir Kalıntı Hastalık: Kuru Tüp Akım Sitometri Metoduyla Gerçek Yaşam Deneyimi. Namık Kemal Tıp Dergisi, vol.10, no.2, 2022, ss.147 - 154. 10.4274/nkmj.galenos.2022.93063 |
| AMA | AKPINAR S,Turgut B Kronik Lenfositik Lösemide Ölçülebilir Kalıntı Hastalık: Kuru Tüp Akım Sitometri Metoduyla Gerçek Yaşam Deneyimi. Namık Kemal Tıp Dergisi. 2022; 10(2): 147 - 154. 10.4274/nkmj.galenos.2022.93063 |
| Vancouver | AKPINAR S,Turgut B Kronik Lenfositik Lösemide Ölçülebilir Kalıntı Hastalık: Kuru Tüp Akım Sitometri Metoduyla Gerçek Yaşam Deneyimi. Namık Kemal Tıp Dergisi. 2022; 10(2): 147 - 154. 10.4274/nkmj.galenos.2022.93063 |
| IEEE | AKPINAR S,Turgut B "Kronik Lenfositik Lösemide Ölçülebilir Kalıntı Hastalık: Kuru Tüp Akım Sitometri Metoduyla Gerçek Yaşam Deneyimi." Namık Kemal Tıp Dergisi, 10, ss.147 - 154, 2022. 10.4274/nkmj.galenos.2022.93063 |
| ISNAD | AKPINAR, SEVAL - Turgut, Burhan. "Kronik Lenfositik Lösemide Ölçülebilir Kalıntı Hastalık: Kuru Tüp Akım Sitometri Metoduyla Gerçek Yaşam Deneyimi". Namık Kemal Tıp Dergisi 10/2 (2022), 147-154. https://doi.org/10.4274/nkmj.galenos.2022.93063 |
