The effect of penicillin-induced epileptiform activity on proinflammatory cytokines levels in the rat brain

FİLİZ, AHMET KEMAL; KARABULUT, SEBAHATTIN

doi:10.17776/csj.775122

The effect of penicillin-induced epileptiform activity on proinflammatory cytokines levels in the rat brain

Ahmet Kemal FİLİZ, (Sivas Cumhuriyet Üniversitesi, Tıp Fakültesi, Fizyoloji Anabilim Dalı, Sivas/ TÜRKİYE)

Sebahattin KARABULUT (Sivas Cumhuriyet Üniversitesi, Sağlık Hizmetleri Meslek Yüksekokulu, Tıbbi Hizmetler ve Teknikler Bölümü, Sivas/ TÜRKİYE)

Cumhuriyet Science Journal

1 1

Yıl: 2021 Cilt: 42 Sayı: 1 Sayfa Aralığı: 1 - 6 Metin Dili: İngilizce DOI: 10.17776/csj.775122 İndeks Tarihi: 13-09-2022

The effect of penicillin-induced epileptiform activity on proinflammatory cytokines levels in the rat brain

Öz:

Emerging evidence indicates a pathogenic role of protracted neuroinflammation in the various neurodegenerative diseases, including epilepsy. Neuroinflammation may contribute to neuronal hyperexcitability underlying seizure formation. The current research aims to examine the changes in the levels of proinflammatory cytokines such as interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α) in the penicillin epilepsy model. In the present study, 12 male Wistar albino rats were randomly divided into two groups as sham and penicillin epilepsy model. Seizures were induced with the intracortical (i.c.) single microinjection 500 IU of penicillin-G into neocortex. Rats were decapitated after observing the cortical epileptic activity and brains were removed by craniotomy. Proinflammatory cytokines (TNF-α and IL-1β) were measured by using ELISA methods in the cortical and hippocampal brain regions. Penicillin significantly up-regulated the expression of IL 1β and TNF-α in the rat cortex, but did not affect the hippocampal cytokines levels. This study is indicative of the neuroinflammatory potential of cortical penicillin administration.

Anahtar Kelime:

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

1] Fisher R.S., Acevedo C., Arzimanoglou A., Bogacz A., Cross J. H., Elger C.E., Engel J., Forsgren L., French J.A., Glynn M., Hesdorffer D.C., Lee B.I., Mathern G.W., Moshé S.L., Perucca E., Scheffer I.E., Tomson T., Watanabe M., Wiebe S., ILAE official report: A Practical Clinical Definition of Epilepsy, Epilepsia, 55(4) (2014) 475-482.
[2] Chen Z., Brodie M.J., Liew D., Kwan P., Treatment Outcomes in Patients with Newly Diagnosed Epilepsy Treated with Established and New Antiepileptic Drugs: A 30-Year Longitudinal Cohort Study, JAMA Neurol., 75(3) (2018) 279-286.
[3] Li Z.H., Li L.L., Jin M.F., Chen X.Q., Sun Q., Ni H., Dysregulation of zinc/lipid metabolism associated genes in the rat hippocampus and cerebral cortex in early adulthood following recurrent neonatal seizures, Mol. Med. Rep., 16(4) (2017) 4701-4709.
[4] Vezzani A., French J., Bartfai T., Baram T.Z., The role of inflammation in epilepsy, Nat Rev Neurol., 7(1) (2011) 31-40.
[5] Li G., Bauer S., Nowak M., Norwood B., Tackenberg, B., Rosenow F., Knake S., Oertel W.H., Hamer H.M., Cytokines and Epilepsy. Seizure, 20(3) (2011) 249-256.
[6] Rawat C., Kukal S., Dahiya U.R., Kukreti R., Cyclooxygenase-2 (COX-2) inhibitors: future therapeutic strategies for epilepsy management, J Neuroinflammation, 16(1) (2019) 1-15.
[7] Sumbul O., Aygun H., The effect of vitamin D3 and paricalcitol on penicillin-induced epileptiform activity in rats., Epilepsy Res., 159 (2020) 106262.
[8] Marangoz A.H., Kocacan S.E., Him A., Kuruoglu E., Cokluk C., Marangoz C., Proconvulsant Effect of Papaverine on Penicillin-Induced Epileptiform Activity in Rats., Turk Neurosurg., 28(3) (2018) 479-482.
[9] Bo G.P., Mainardi P., Benassi E., Besio G., Faverio A., Scotto P.A., Loeb C., Parenteral penicillin model of epilepsy in the rat: a reappraisal, Methods Find Exp. Clin. Pharmacol., 8(8) (1986) 491-496.
[10] Paxinos G., Watson C., The Rat Brain in Stereotaxic Coordinates, 6th ed., Amsterdam: Elsevier Academic Press, (2007).
[11] Kruger N.J., The Bradford method for protein quantitation, Methods Mol. Biol., 32 (1994) 9-15. [12] Webster K.M., Sun M., Crack P., O'Brien T.J., Shultz S.R., Semple B.D., Inflammation in epileptogenesis after traumatic brain injury, J. Neuroinflammation, 14(1) (2017) 10.
[13] Robel S., Buckingham S.C., Boni J.L., Campbell S.L., Danbolt N.C., Riedemann T., Sutor B., Sontheimer H., Reactive astrogliosis causes the development of spontaneous seizures, J Neurosci., 35(8) (2015) 3330-3345.
[14] Eyo U.B., Murugan M., Wu L.J., Microglia- neuron communication in epilepsy, Glia, 65 (2017) 5–18. [15] Dinarello C.A., Biologic basis for interleukin-1 in disease, Blood, 87(6) (1996) 2095–2147.
[16] De Simoni M.G., Perego C., Ravizza T., Moneta D., Conti M., Marchesi F., De Luigi A., Garattini S., Vezzani A., Inflammatory cytokines and related genes are induced in the rat hippocampus by limbic status epilepticus, Eur. J. Neurosci., 12(7) (2000) 2623-2633.
[17] Wallenstein M.C., Attenuation of penicillin models of epilepsy by nonsteroidal anti- inflammatory drugs, Exp Neurol., 98(1) (1987) 152-160.
[18] Zhu X., Chen Y., Du Y., Wan Q., Xu Y., Wu J., Astragaloside IV attenuates penicillin-induced epilepsy via inhibiting activation of the MAPK signaling pathway, Mol. Med. Rep., 17(1) (2018) 643-647.
[19] Taşkıran AŞ., Taştemur Y., The comparison of inflammatory markers in pentylenetetrazole- induced acute epileptic seizure model and chronic epilepsy model in rats., Cumhuriyet Sci. J., 41(3) (2020) 635-641.
[20] Devinsky O., Vezzani A., O'Brien T.J., Jette N., Scheffer I.E., de Curtis M., Perucca P., Epilepsy, Nat. Rev. Dis. Primers, 4 (2018) 18024.
[21] Balosso S., Ravizza T., Perego C., Peschon J., Campbell I.L., De Simoni M.G., Vezzani A., Tumor necrosis factor-alpha inhibits seizures in mice via p75 receptors, Ann. Neurol., 57(6) (2005) 804-812.
[22] Shandra A.A., Godlevsky L.S., Vastyanov R.S., Oleinik A.A., Konovalenko V.L., Rapoport E.N., Korobka N.N., The role of TNF-alpha in amygdala kindled rats, Neurosci Res., 42(2) (2002) 147-153.

APA	FİLİZ A, KARABULUT S (2021). The effect of penicillin-induced epileptiform activity on proinflammatory cytokines levels in the rat brain. , 1 - 6. 10.17776/csj.775122
Chicago	FİLİZ AHMET KEMAL,KARABULUT SEBAHATTIN The effect of penicillin-induced epileptiform activity on proinflammatory cytokines levels in the rat brain. (2021): 1 - 6. 10.17776/csj.775122
MLA	FİLİZ AHMET KEMAL,KARABULUT SEBAHATTIN The effect of penicillin-induced epileptiform activity on proinflammatory cytokines levels in the rat brain. , 2021, ss.1 - 6. 10.17776/csj.775122
AMA	FİLİZ A,KARABULUT S The effect of penicillin-induced epileptiform activity on proinflammatory cytokines levels in the rat brain. . 2021; 1 - 6. 10.17776/csj.775122
Vancouver	FİLİZ A,KARABULUT S The effect of penicillin-induced epileptiform activity on proinflammatory cytokines levels in the rat brain. . 2021; 1 - 6. 10.17776/csj.775122
IEEE	FİLİZ A,KARABULUT S "The effect of penicillin-induced epileptiform activity on proinflammatory cytokines levels in the rat brain." , ss.1 - 6, 2021. 10.17776/csj.775122
ISNAD	FİLİZ, AHMET KEMAL - KARABULUT, SEBAHATTIN. "The effect of penicillin-induced epileptiform activity on proinflammatory cytokines levels in the rat brain". (2021), 1-6. https://doi.org/10.17776/csj.775122

APA	FİLİZ A, KARABULUT S (2021). The effect of penicillin-induced epileptiform activity on proinflammatory cytokines levels in the rat brain. Cumhuriyet Science Journal, 42(1), 1 - 6. 10.17776/csj.775122
Chicago	FİLİZ AHMET KEMAL,KARABULUT SEBAHATTIN The effect of penicillin-induced epileptiform activity on proinflammatory cytokines levels in the rat brain. Cumhuriyet Science Journal 42, no.1 (2021): 1 - 6. 10.17776/csj.775122
MLA	FİLİZ AHMET KEMAL,KARABULUT SEBAHATTIN The effect of penicillin-induced epileptiform activity on proinflammatory cytokines levels in the rat brain. Cumhuriyet Science Journal, vol.42, no.1, 2021, ss.1 - 6. 10.17776/csj.775122
AMA	FİLİZ A,KARABULUT S The effect of penicillin-induced epileptiform activity on proinflammatory cytokines levels in the rat brain. Cumhuriyet Science Journal. 2021; 42(1): 1 - 6. 10.17776/csj.775122
Vancouver	FİLİZ A,KARABULUT S The effect of penicillin-induced epileptiform activity on proinflammatory cytokines levels in the rat brain. Cumhuriyet Science Journal. 2021; 42(1): 1 - 6. 10.17776/csj.775122
IEEE	FİLİZ A,KARABULUT S "The effect of penicillin-induced epileptiform activity on proinflammatory cytokines levels in the rat brain." Cumhuriyet Science Journal, 42, ss.1 - 6, 2021. 10.17776/csj.775122
ISNAD	FİLİZ, AHMET KEMAL - KARABULUT, SEBAHATTIN. "The effect of penicillin-induced epileptiform activity on proinflammatory cytokines levels in the rat brain". Cumhuriyet Science Journal 42/1 (2021), 1-6. https://doi.org/10.17776/csj.775122